Pharmacomechanical coupling in rat vas deferens: Effects of agents that modulate intracellular release of calcium and protein kinase C activation
Identifieur interne : 003830 ( Main/Exploration ); précédent : 003829; suivant : 003831Pharmacomechanical coupling in rat vas deferens: Effects of agents that modulate intracellular release of calcium and protein kinase C activation
Auteurs : N. I. B. Amobi [Royaume-Uni] ; D. Sugden [Royaume-Uni] ; I. C. H. Smith [Royaume-Uni]Source :
- Life Sciences [ 0024-3205 ] ; 1999.
English descriptors
- Teeft :
- Agonist, Biol, Calcium release, Calcium stores, Calphostin, Chem, Contractile, Cumulative additions, Cyclopiazonic acid, Deferens, Drug addition, Epididymal, Epididymal portion, Ester, Inhibitor, Intracellular, Intracellular release, Isoform, Isoforms, Kinase, Krebs, Mrna, Noradrenaline, Normal krebs, Pathway, Pdbu, Pflugers arch, Pharmacol, Phorbol, Phorbol esters, Physiol, Possible involvement, Present study, Primer, Procaine, Protein kinase, Ryanodine, Sequential contractions, Somlyo, Thapsigargin.
Abstract
Abstract: The effects of agents that modulate intracellular release of calcium and protein kinase C (PKC) activation on noradrenaline (NA)-induced contractions of epididymal vas deferens in calcium-free/EGTA (1 mM) medium were investigated. NA (100 μM) or methoxamine (100 μM) evoked repeatable contractions. Clonidine (100–300 μM) was ineffective. The contractions to NA were reduced by procaine (1–10 mM) but not by thapsigargin (0.1–30 μM), ryanodine (1–30 μM) or TMB-8 (1–30 μM). Contractions to cumulative additions of NA (1–100 μM) were enhanced in the presence of cyclopiazonic acid (10 & 30 μM) but not ryanodine (10 & 30 μM). Sequential contractions to NA were not blocked by PKC inhibitors, calphostin C (1 μM) or Ro 31-8220 (1–30 μM) but were reduced by H-7 (1–30 μM), a broad spectrum protein kinase inhibitor. Although RT-PCR experiments detected mRNA for some Ca2+-dependent/DAG-activated and Ca2+-independent/DAG-activated PKC isoforms in epididymal vas deferens, the PKC activators, phorbol 12, 13-dibutyrate (100 μM) or phorbol 12-myristate 13-acetate (100 μM) failed to activate the tissues in calcium-free medium but enhanced subsequent contractions to NA. These results indicate a limited role for intracellular calcium stores and phorbol ester/DAG-sensitive PKC isoforms in NA-induced contraction of epididymal rat vas deferens in calcium-free medium. The results suggest that pharmacomechanical coupling triggered by NA may involve the sensitization of contractile myofilaments to Ca2+ or a Ca2+-independent mechanism. The possible involvement of Ca2+-independent/DAG-insensitive PKC isoforms and agonist-dependent but PKC-independent sensitization pathway is discussed.
Url:
DOI: 10.1016/S0024-3205(99)00231-3
Affiliations:
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I. B." last="Amobi">N. I. B. Amobi</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Physiology Division, King's College London, Campden Hill Road, London W8 7AH</wicri:regionArea><wicri:noRegion>London W8 7AH</wicri:noRegion></affiliation></author><author><name sortKey="Sugden, D" sort="Sugden, D" uniqKey="Sugden D" first="D." last="Sugden">D. Sugden</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Physiology Division, King's College London, Campden Hill Road, London W8 7AH</wicri:regionArea><wicri:noRegion>London W8 7AH</wicri:noRegion></affiliation></author><author><name sortKey="Smith, I C H" sort="Smith, I C H" uniqKey="Smith I" first="I. C. H." last="Smith">I. C. H. Smith</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Physiology Division, King's College London, Campden Hill Road, London W8 7AH</wicri:regionArea><wicri:noRegion>London W8 7AH</wicri:noRegion></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Life Sciences</title><title level="j" type="abbrev">LFS</title><idno type="ISSN">0024-3205</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">65</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="145">145</biblScope><biblScope unit="page" to="156">156</biblScope></imprint><idno type="ISSN">0024-3205</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0024-3205</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Agonist</term><term>Biol</term><term>Calcium release</term><term>Calcium stores</term><term>Calphostin</term><term>Chem</term><term>Contractile</term><term>Cumulative additions</term><term>Cyclopiazonic acid</term><term>Deferens</term><term>Drug addition</term><term>Epididymal</term><term>Epididymal portion</term><term>Ester</term><term>Inhibitor</term><term>Intracellular</term><term>Intracellular release</term><term>Isoform</term><term>Isoforms</term><term>Kinase</term><term>Krebs</term><term>Mrna</term><term>Noradrenaline</term><term>Normal krebs</term><term>Pathway</term><term>Pdbu</term><term>Pflugers arch</term><term>Pharmacol</term><term>Phorbol</term><term>Phorbol esters</term><term>Physiol</term><term>Possible involvement</term><term>Present study</term><term>Primer</term><term>Procaine</term><term>Protein kinase</term><term>Ryanodine</term><term>Sequential contractions</term><term>Somlyo</term><term>Thapsigargin</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The effects of agents that modulate intracellular release of calcium and protein kinase C (PKC) activation on noradrenaline (NA)-induced contractions of epididymal vas deferens in calcium-free/EGTA (1 mM) medium were investigated. NA (100 μM) or methoxamine (100 μM) evoked repeatable contractions. Clonidine (100–300 μM) was ineffective. The contractions to NA were reduced by procaine (1–10 mM) but not by thapsigargin (0.1–30 μM), ryanodine (1–30 μM) or TMB-8 (1–30 μM). Contractions to cumulative additions of NA (1–100 μM) were enhanced in the presence of cyclopiazonic acid (10 & 30 μM) but not ryanodine (10 & 30 μM). Sequential contractions to NA were not blocked by PKC inhibitors, calphostin C (1 μM) or Ro 31-8220 (1–30 μM) but were reduced by H-7 (1–30 μM), a broad spectrum protein kinase inhibitor. Although RT-PCR experiments detected mRNA for some Ca2+-dependent/DAG-activated and Ca2+-independent/DAG-activated PKC isoforms in epididymal vas deferens, the PKC activators, phorbol 12, 13-dibutyrate (100 μM) or phorbol 12-myristate 13-acetate (100 μM) failed to activate the tissues in calcium-free medium but enhanced subsequent contractions to NA. These results indicate a limited role for intracellular calcium stores and phorbol ester/DAG-sensitive PKC isoforms in NA-induced contraction of epididymal rat vas deferens in calcium-free medium. The results suggest that pharmacomechanical coupling triggered by NA may involve the sensitization of contractile myofilaments to Ca2+ or a Ca2+-independent mechanism. The possible involvement of Ca2+-independent/DAG-insensitive PKC isoforms and agonist-dependent but PKC-independent sensitization pathway is discussed.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Amobi, N I B" sort="Amobi, N I B" uniqKey="Amobi N" first="N. I. B." last="Amobi">N. I. B. Amobi</name></noRegion><name sortKey="Smith, I C H" sort="Smith, I C H" uniqKey="Smith I" first="I. C. H." last="Smith">I. C. H. Smith</name><name sortKey="Sugden, D" sort="Sugden, D" uniqKey="Sugden D" first="D." last="Sugden">D. Sugden</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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