Cyclic AMP stimulates the gene expression of a non-selective cation channel, mNSC1, in pancreatic β-cell line, MIN6
Identifieur interne : 003750 ( Main/Exploration ); précédent : 003749; suivant : 003751Cyclic AMP stimulates the gene expression of a non-selective cation channel, mNSC1, in pancreatic β-cell line, MIN6
Auteurs : Jun-Ichi Satoh [Japon] ; Keiko Kutsuwada [Japon] ; Gaku Ohki [Japon] ; Masashi Imai [Japon] ; Masayuki Kobayashi [Japon] ; Makoto Suzuki [Japon]Source :
- Molecular and Cellular Endocrinology [ 0303-7207 ] ; 2000.
English descriptors
- KwdEn :
- Teeft :
- Actinomycin, Assay, Biol, Biophys, Cation, Cation channel, Cell lysate, Cellular endocrinology, Chain reaction, Chem, Competitive template, Copy number, Dibutyryl, Dibutyryl camp, Endocrinology, Gene expression, Glucose, Grand island, Incubation, Insulin, Insulin release, Insulin secretion, Leech, Luciferase, Lysis buffer, Min6, Min6 cells, Mnsc1, Mnsc1 gene, Mnsc1 mrna, Mnsc1 protein, Mrna, Mrna level, Mrna stability, Pancreatic, Pancreatic line, Protein kinase, Reaction mixture, Respective incubation time, Satoh, Southern blot analysis, Western blot analysis.
Abstract
Abstract: Mouse non-selective cation channel 1 (mNSC 1) cDNA from mouse pancreatic β-cell line, MIN6, have recently been cloned. Since the number of non-selective cation channel in pancreatic duct cells has been reported to increase 9-fold in 5 h incubation with cAMP, the effect of cAMP on the gene expression of mNSC1 in MIN6 cells was examined. Dibutyryl cAMP (db-cAMP) was shown to increase the level of the mRNA by reverse transcription-polymerase chain reaction (RT-PCR). The copy number of the mRNA was increased 4-fold in 6 h incubation with db-cAMP by competitive PCR. Western blot analysis also indicated a 4-fold increase in the quantity of the newly synthesized protein in 9 h incubation with db-cAMP. Experiments with 5′-flanking region and with a transcriptional inhibitor suggested that db-cAMP affected transcription, and protected the mRNA from its degradation as well. It is concluded that the expression of mNSC1 is indeed increased by cAMP in the pancreatic β-cells.
Url:
DOI: 10.1016/S0303-7207(99)00214-2
Affiliations:
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Tochigi 329-0498</wicri:regionArea><wicri:noRegion>Tochigi 329-0498</wicri:noRegion></affiliation></author><author><name sortKey="Ohki, Gaku" sort="Ohki, Gaku" uniqKey="Ohki G" first="Gaku" last="Ohki">Gaku Ohki</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pharmacology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi, Kawachi, Tochigi 329-0498</wicri:regionArea><wicri:noRegion>Tochigi 329-0498</wicri:noRegion></affiliation></author><author><name sortKey="Imai, Masashi" sort="Imai, Masashi" uniqKey="Imai M" first="Masashi" last="Imai">Masashi Imai</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pharmacology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi, Kawachi, Tochigi 329-0498</wicri:regionArea><wicri:noRegion>Tochigi 329-0498</wicri:noRegion></affiliation></author><author><name sortKey="Kobayashi, Masayuki" sort="Kobayashi, Masayuki" uniqKey="Kobayashi M" first="Masayuki" last="Kobayashi">Masayuki Kobayashi</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of General Internal Medicine, Kashiwa Hospital, Jikei University School of Medicine, 163-1 Kashiwashita, Kashiwa, Chiba 277-0004</wicri:regionArea><wicri:noRegion>Chiba 277-0004</wicri:noRegion></affiliation></author><author><name sortKey="Suzuki, Makoto" sort="Suzuki, Makoto" uniqKey="Suzuki M" first="Makoto" last="Suzuki">Makoto Suzuki</name><affiliation wicri:level="1"><country wicri:rule="url">Japon</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pharmacology, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi, Kawachi, Tochigi 329-0498</wicri:regionArea><wicri:noRegion>Tochigi 329-0498</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular and Cellular Endocrinology</title><title level="j" type="abbrev">MCE</title><idno type="ISSN">0303-7207</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">160</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="165">165</biblScope><biblScope unit="page" to="171">171</biblScope></imprint><idno type="ISSN">0303-7207</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0303-7207</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene expression</term><term>Non-selective cation channel</term><term>Pancreatic β-cell</term><term>cAMP</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Actinomycin</term><term>Assay</term><term>Biol</term><term>Biophys</term><term>Cation</term><term>Cation channel</term><term>Cell lysate</term><term>Cellular endocrinology</term><term>Chain reaction</term><term>Chem</term><term>Competitive template</term><term>Copy number</term><term>Dibutyryl</term><term>Dibutyryl camp</term><term>Endocrinology</term><term>Gene expression</term><term>Glucose</term><term>Grand island</term><term>Incubation</term><term>Insulin</term><term>Insulin release</term><term>Insulin secretion</term><term>Leech</term><term>Luciferase</term><term>Lysis buffer</term><term>Min6</term><term>Min6 cells</term><term>Mnsc1</term><term>Mnsc1 gene</term><term>Mnsc1 mrna</term><term>Mnsc1 protein</term><term>Mrna</term><term>Mrna level</term><term>Mrna stability</term><term>Pancreatic</term><term>Pancreatic line</term><term>Protein kinase</term><term>Reaction mixture</term><term>Respective incubation time</term><term>Satoh</term><term>Southern blot analysis</term><term>Western blot analysis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Mouse non-selective cation channel 1 (mNSC 1) cDNA from mouse pancreatic β-cell line, MIN6, have recently been cloned. Since the number of non-selective cation channel in pancreatic duct cells has been reported to increase 9-fold in 5 h incubation with cAMP, the effect of cAMP on the gene expression of mNSC1 in MIN6 cells was examined. Dibutyryl cAMP (db-cAMP) was shown to increase the level of the mRNA by reverse transcription-polymerase chain reaction (RT-PCR). The copy number of the mRNA was increased 4-fold in 6 h incubation with db-cAMP by competitive PCR. Western blot analysis also indicated a 4-fold increase in the quantity of the newly synthesized protein in 9 h incubation with db-cAMP. Experiments with 5′-flanking region and with a transcriptional inhibitor suggested that db-cAMP affected transcription, and protected the mRNA from its degradation as well. It is concluded that the expression of mNSC1 is indeed increased by cAMP in the pancreatic β-cells.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Satoh, Jun Ichi" sort="Satoh, Jun Ichi" uniqKey="Satoh J" first="Jun-Ichi" last="Satoh">Jun-Ichi Satoh</name></noRegion><name sortKey="Imai, Masashi" sort="Imai, Masashi" uniqKey="Imai M" first="Masashi" last="Imai">Masashi Imai</name><name sortKey="Kobayashi, Masayuki" sort="Kobayashi, Masayuki" uniqKey="Kobayashi M" first="Masayuki" last="Kobayashi">Masayuki Kobayashi</name><name sortKey="Kutsuwada, Keiko" sort="Kutsuwada, Keiko" uniqKey="Kutsuwada K" first="Keiko" last="Kutsuwada">Keiko Kutsuwada</name><name sortKey="Ohki, Gaku" sort="Ohki, Gaku" uniqKey="Ohki G" first="Gaku" last="Ohki">Gaku Ohki</name><name sortKey="Suzuki, Makoto" sort="Suzuki, Makoto" uniqKey="Suzuki M" first="Makoto" last="Suzuki">Makoto Suzuki</name><name sortKey="Suzuki, Makoto" sort="Suzuki, Makoto" uniqKey="Suzuki M" first="Makoto" last="Suzuki">Makoto Suzuki</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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