Selection of homeotic proteins for binding to a human DNA replication origin
Identifieur interne : 003699 ( Main/Exploration ); précédent : 003698; suivant : 003700Selection of homeotic proteins for binding to a human DNA replication origin
Auteurs : Elisa De Stanchina [Italie] ; Davide Gabellini [Italie] ; Paolo Norio [Italie] ; Mauro Giacca [Italie] ; Fiorenzo A. Peverali [Italie] ; Silvano Riva [Italie] ; Arturo Falaschi [Italie] ; Giuseppe Biamonti [Italie]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 2000.
English descriptors
- KwdEn :
- Teeft :
- Abdurashidova, Academic press, Accession, Accession number, Activation, Activation domain, Amino, Amino acid residues, Assay, Biamonti, Binding site, Binding sites, Biol, Catalogue number, Catase activity, Cdna, Cdna probes, Cell biol, Cell cycle, Cell extracts, Cell lines, Chloramphenicol acetyl transferase, Chromatin structure, Clone, Codon, Cold spring harbor, Cold spring harbor laboratory, Cold spring harbor laboratory press, Coli, Developmental regulators, Different portions, Dimitrova, Donaldson blow, Dutta bell, Electrophoretic mobility shift assay, Emsa, Escherichia coli, Eukaryotic, Eukaryotic cells, Footprinting, Footprinting experiments, Fusion protein, Genbank, Genbank accession number, Gene, Genetic analysis, Genomic, Genomic clone, Giacca, Hela, Hela cells, High level, Homeobox, Homeodomain, Homeodomain proteins, Homeogene family, Homeoproteins, Homeotic genes, Human lamin, Hybrid protein, Hybrid proteins, Kpni site, Lacz gene, Lamin, Mammalian cells, Marahrens stillman, Metazoan cells, Minutes incubation, Molar, Natl acad, Onehybrid screening, Open reading frame, Origin activation, Origin area, Origin figure, Origin recognition, Origin region, Origin sequence, Other hand, Other proteins, Partial cdna, Plasmid, Possible role, Ppv1, Ppv1 gene, Ppv1 gene promoter, Proliferative stimuli, Promoter, Protein, Rabbit polyclonal, Recombinant protein, Recombinant proteins, Replication, Replication origin, Replication origins, Reporter gene, Riva, Room temperature, Saccharomyces cerevisiae, Same extracts, Same orientation, Same unlabelled oligo, Serum starvation, Simian virus, Single intron, Standard methods, Stillman, Stimulatory effect, Total proteins, Transcription, Transcription factor, Transcription factors, Transcriptional, Trends biochem, Untranslated region, Ura3 locus, Vivo, Vivo footprinting analysis, Yeast, Yeast cells, Yeast origins, Yeast replication origins, Yeast strain.
Abstract
Abstract: We have previously shown that a cell cycle-dependent nucleoprotein complex assembles in vivo on a 74 bp sequence within the human DNA replication origin associated to the Lamin B2 gene. Here, we report the identification, using a one-hybrid screen in yeast, of three proteins interacting with the 74 bp sequence. All of them, namely HOXA13, HOXC10 and HOXC13, are orthologues of the Abdominal-B gene of Drosophila melanogaster and are members of the homeogene family of developmental regulators. We describe the complete open reading frame sequence of HOXC10 and HOXC13 along with the structure of the HoxC13 gene. The specificity of binding of these two proteins to the Lamin B2 origin is confirmed by both band-shift and in vitro footprinting assays. In addition, the ability of HOXC10 and HOXC13 to increase the activity of a promoter containing the 74 bp sequence, as assayed by CAT-assay experiments, demonstrates a direct interaction of these homeoproteins with the origin sequence in mammalian cells. We also show that HOXC10 expression is cell-type-dependent and positively correlates with cell proliferation.
Url:
DOI: 10.1006/jmbi.2000.3782
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001852
- to stream Istex, to step Curation: 001852
- to stream Istex, to step Checkpoint: 000F98
- to stream Main, to step Merge: 003741
- to stream Main, to step Curation: 003699
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Selection of homeotic proteins for binding to a human DNA replication origin</title><author><name sortKey="De Stanchina, Elisa" sort="De Stanchina, Elisa" uniqKey="De Stanchina E" first="Elisa" last="De Stanchina">Elisa De Stanchina</name></author><author><name sortKey="Gabellini, Davide" sort="Gabellini, Davide" uniqKey="Gabellini D" first="Davide" last="Gabellini">Davide Gabellini</name></author><author><name sortKey="Norio, Paolo" sort="Norio, Paolo" uniqKey="Norio P" first="Paolo" last="Norio">Paolo Norio</name></author><author><name sortKey="Giacca, Mauro" sort="Giacca, Mauro" uniqKey="Giacca M" first="Mauro" last="Giacca">Mauro Giacca</name></author><author><name sortKey="Peverali, Fiorenzo A" sort="Peverali, Fiorenzo A" uniqKey="Peverali F" first="Fiorenzo A" last="Peverali">Fiorenzo A. Peverali</name></author><author><name sortKey="Riva, Silvano" sort="Riva, Silvano" uniqKey="Riva S" first="Silvano" last="Riva">Silvano Riva</name></author><author><name sortKey="Falaschi, Arturo" sort="Falaschi, Arturo" uniqKey="Falaschi A" first="Arturo" last="Falaschi">Arturo Falaschi</name></author><author><name sortKey="Biamonti, Giuseppe" sort="Biamonti, Giuseppe" uniqKey="Biamonti G" first="Giuseppe" last="Biamonti">Giuseppe Biamonti</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0CCB1215FCD742084270E7AD438918B3D149F1B4</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1006/jmbi.2000.3782</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-V2HQS6HC-C/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001852</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001852</idno><idno type="wicri:Area/Istex/Curation">001852</idno><idno type="wicri:Area/Istex/Checkpoint">000F98</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F98</idno><idno type="wicri:doubleKey">0022-2836:2000:De Stanchina E:selection:of:homeotic</idno><idno type="wicri:Area/Main/Merge">003741</idno><idno type="wicri:Area/Main/Curation">003699</idno><idno type="wicri:Area/Main/Exploration">003699</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Selection of homeotic proteins for binding to a human DNA replication origin</title><author><name sortKey="De Stanchina, Elisa" sort="De Stanchina, Elisa" uniqKey="De Stanchina E" first="Elisa" last="De Stanchina">Elisa De Stanchina</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto di Genetica Biochimica ed Evoluzionistica del CNR Via Abbiategrasso 207 27100, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Gabellini, Davide" sort="Gabellini, Davide" uniqKey="Gabellini D" first="Davide" last="Gabellini">Davide Gabellini</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto di Genetica Biochimica ed Evoluzionistica del CNR Via Abbiategrasso 207 27100, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Norio, Paolo" sort="Norio, Paolo" uniqKey="Norio P" first="Paolo" last="Norio">Paolo Norio</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>International Center for Genetic Engineering and Biotechnology, Padriciano 99 34012 Trieste</wicri:regionArea><wicri:noRegion>Padriciano 99 34012 Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Giacca, Mauro" sort="Giacca, Mauro" uniqKey="Giacca M" first="Mauro" last="Giacca">Mauro Giacca</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto di Genetica Biochimica ed Evoluzionistica del CNR Via Abbiategrasso 207 27100, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Peverali, Fiorenzo A" sort="Peverali, Fiorenzo A" uniqKey="Peverali F" first="Fiorenzo A" last="Peverali">Fiorenzo A. Peverali</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto di Genetica Biochimica ed Evoluzionistica del CNR Via Abbiategrasso 207 27100, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Riva, Silvano" sort="Riva, Silvano" uniqKey="Riva S" first="Silvano" last="Riva">Silvano Riva</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto di Genetica Biochimica ed Evoluzionistica del CNR Via Abbiategrasso 207 27100, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author><author><name sortKey="Falaschi, Arturo" sort="Falaschi, Arturo" uniqKey="Falaschi A" first="Arturo" last="Falaschi">Arturo Falaschi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>International Center for Genetic Engineering and Biotechnology, Padriciano 99 34012 Trieste</wicri:regionArea><wicri:noRegion>Padriciano 99 34012 Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Biamonti, Giuseppe" sort="Biamonti, Giuseppe" uniqKey="Biamonti G" first="Giuseppe" last="Biamonti">Giuseppe Biamonti</name><affiliation wicri:level="1"><country wicri:rule="url">Italie</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto di Genetica Biochimica ed Evoluzionistica del CNR Via Abbiategrasso 207 27100, Pavia</wicri:regionArea><wicri:noRegion>Pavia</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Molecular Biology</title><title level="j" type="abbrev">YJMBI</title><idno type="ISSN">0022-2836</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">299</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="667">667</biblScope><biblScope unit="page" to="680">680</biblScope></imprint><idno type="ISSN">0022-2836</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-2836</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3′-UTR</term><term>CAT</term><term>DNA replication origin</term><term>EMSA</term><term>Lamin B2</term><term>ORC</term><term>ORF</term><term>homeoproteins</term><term>one-hybrid</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Abdurashidova</term><term>Academic press</term><term>Accession</term><term>Accession number</term><term>Activation</term><term>Activation domain</term><term>Amino</term><term>Amino acid residues</term><term>Assay</term><term>Biamonti</term><term>Binding site</term><term>Binding sites</term><term>Biol</term><term>Catalogue number</term><term>Catase activity</term><term>Cdna</term><term>Cdna probes</term><term>Cell biol</term><term>Cell cycle</term><term>Cell extracts</term><term>Cell lines</term><term>Chloramphenicol acetyl transferase</term><term>Chromatin structure</term><term>Clone</term><term>Codon</term><term>Cold spring harbor</term><term>Cold spring harbor laboratory</term><term>Cold spring harbor laboratory press</term><term>Coli</term><term>Developmental regulators</term><term>Different portions</term><term>Dimitrova</term><term>Donaldson blow</term><term>Dutta bell</term><term>Electrophoretic mobility shift assay</term><term>Emsa</term><term>Escherichia coli</term><term>Eukaryotic</term><term>Eukaryotic cells</term><term>Footprinting</term><term>Footprinting experiments</term><term>Fusion protein</term><term>Genbank</term><term>Genbank accession number</term><term>Gene</term><term>Genetic analysis</term><term>Genomic</term><term>Genomic clone</term><term>Giacca</term><term>Hela</term><term>Hela cells</term><term>High level</term><term>Homeobox</term><term>Homeodomain</term><term>Homeodomain proteins</term><term>Homeogene family</term><term>Homeoproteins</term><term>Homeotic genes</term><term>Human lamin</term><term>Hybrid protein</term><term>Hybrid proteins</term><term>Kpni site</term><term>Lacz gene</term><term>Lamin</term><term>Mammalian cells</term><term>Marahrens stillman</term><term>Metazoan cells</term><term>Minutes incubation</term><term>Molar</term><term>Natl acad</term><term>Onehybrid screening</term><term>Open reading frame</term><term>Origin activation</term><term>Origin area</term><term>Origin figure</term><term>Origin recognition</term><term>Origin region</term><term>Origin sequence</term><term>Other hand</term><term>Other proteins</term><term>Partial cdna</term><term>Plasmid</term><term>Possible role</term><term>Ppv1</term><term>Ppv1 gene</term><term>Ppv1 gene promoter</term><term>Proliferative stimuli</term><term>Promoter</term><term>Protein</term><term>Rabbit polyclonal</term><term>Recombinant protein</term><term>Recombinant proteins</term><term>Replication</term><term>Replication origin</term><term>Replication origins</term><term>Reporter gene</term><term>Riva</term><term>Room temperature</term><term>Saccharomyces cerevisiae</term><term>Same extracts</term><term>Same orientation</term><term>Same unlabelled oligo</term><term>Serum starvation</term><term>Simian virus</term><term>Single intron</term><term>Standard methods</term><term>Stillman</term><term>Stimulatory effect</term><term>Total proteins</term><term>Transcription</term><term>Transcription factor</term><term>Transcription factors</term><term>Transcriptional</term><term>Trends biochem</term><term>Untranslated region</term><term>Ura3 locus</term><term>Vivo</term><term>Vivo footprinting analysis</term><term>Yeast</term><term>Yeast cells</term><term>Yeast origins</term><term>Yeast replication origins</term><term>Yeast strain</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We have previously shown that a cell cycle-dependent nucleoprotein complex assembles in vivo on a 74 bp sequence within the human DNA replication origin associated to the Lamin B2 gene. Here, we report the identification, using a one-hybrid screen in yeast, of three proteins interacting with the 74 bp sequence. All of them, namely HOXA13, HOXC10 and HOXC13, are orthologues of the Abdominal-B gene of Drosophila melanogaster and are members of the homeogene family of developmental regulators. We describe the complete open reading frame sequence of HOXC10 and HOXC13 along with the structure of the HoxC13 gene. The specificity of binding of these two proteins to the Lamin B2 origin is confirmed by both band-shift and in vitro footprinting assays. In addition, the ability of HOXC10 and HOXC13 to increase the activity of a promoter containing the 74 bp sequence, as assayed by CAT-assay experiments, demonstrates a direct interaction of these homeoproteins with the origin sequence in mammalian cells. We also show that HOXC10 expression is cell-type-dependent and positively correlates with cell proliferation.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="De Stanchina, Elisa" sort="De Stanchina, Elisa" uniqKey="De Stanchina E" first="Elisa" last="De Stanchina">Elisa De Stanchina</name></noRegion><name sortKey="Biamonti, Giuseppe" sort="Biamonti, Giuseppe" uniqKey="Biamonti G" first="Giuseppe" last="Biamonti">Giuseppe Biamonti</name><name sortKey="Biamonti, Giuseppe" sort="Biamonti, Giuseppe" uniqKey="Biamonti G" first="Giuseppe" last="Biamonti">Giuseppe Biamonti</name><name sortKey="Falaschi, Arturo" sort="Falaschi, Arturo" uniqKey="Falaschi A" first="Arturo" last="Falaschi">Arturo Falaschi</name><name sortKey="Gabellini, Davide" sort="Gabellini, Davide" uniqKey="Gabellini D" first="Davide" last="Gabellini">Davide Gabellini</name><name sortKey="Giacca, Mauro" sort="Giacca, Mauro" uniqKey="Giacca M" first="Mauro" last="Giacca">Mauro Giacca</name><name sortKey="Norio, Paolo" sort="Norio, Paolo" uniqKey="Norio P" first="Paolo" last="Norio">Paolo Norio</name><name sortKey="Peverali, Fiorenzo A" sort="Peverali, Fiorenzo A" uniqKey="Peverali F" first="Fiorenzo A" last="Peverali">Fiorenzo A. Peverali</name><name sortKey="Riva, Silvano" sort="Riva, Silvano" uniqKey="Riva S" first="Silvano" last="Riva">Silvano Riva</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003699 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003699 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:0CCB1215FCD742084270E7AD438918B3D149F1B4
|texte= Selection of homeotic proteins for binding to a human DNA replication origin
}}
| This area was generated with Dilib version V0.6.33. |  |