Fluorescent pteridine nucleoside analogs
Identifieur interne : 003560 ( Main/Exploration ); précédent : 003559; suivant : 003561Fluorescent pteridine nucleoside analogs
Auteurs : Mary E. Hawkins [États-Unis]Source :
- Cell Biochemistry and Biophysics [ 1085-9195 ] ; 2001-04-01.
Abstract
Abstract: Pteridine nucleoside analog probes are highly fluorescent and offer different approaches to monitor subtle DNA interactions with other molecules. Similarities in structure and size to native nucleosides make it possible to incorporate these probes into oligonucleotides through the standard deoxyribose linkage. These probes are formulated as phosphoramidites and incorporated into oligonucleotides using automated DNA synthesis. Their position within the oligonucleotide renders them exquisitely sensitive to changes in structure as the oligonucleotide meets and reacts with other molecules. Changes are measured through fluorescence intensity, anisotropy, lifetimes, spectral shifts, and energy transfer. The fluorescence properties of pteridine nucleoside analogs as monomers and incorporated into single and double stranded oligonucleotides are reviewed. The two guanosine analogs, 3MI and 6MI, and two adenosine analogs, 6MAP and DMAP, are reviewed in detail along with applications utilizing them.
Url:
DOI: 10.1385/CBB:34:2:257
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002292
- to stream Istex, to step Curation: 002292
- to stream Istex, to step Checkpoint: 000E82
- to stream Main, to step Merge: 003598
- to stream Main, to step Curation: 003560
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fluorescent pteridine nucleoside analogs</title>
<author><name sortKey="Hawkins, Mary E" sort="Hawkins, Mary E" uniqKey="Hawkins M" first="Mary E." last="Hawkins">Mary E. Hawkins</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:0ACDFD8F889CC4D53DC53B25F43DEA4B18CD03A4</idno>
<date when="2001" year="2001">2001</date>
<idno type="doi">10.1385/CBB:34:2:257</idno>
<idno type="url">https://api.istex.fr/ark:/67375/VQC-QJJ19DVF-M/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002292</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002292</idno>
<idno type="wicri:Area/Istex/Curation">002292</idno>
<idno type="wicri:Area/Istex/Checkpoint">000E82</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000E82</idno>
<idno type="wicri:doubleKey">1085-9195:2001:Hawkins M:fluorescent:pteridine:nucleoside</idno>
<idno type="wicri:Area/Main/Merge">003598</idno>
<idno type="wicri:Area/Main/Curation">003560</idno>
<idno type="wicri:Area/Main/Exploration">003560</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Fluorescent pteridine nucleoside analogs</title>
<author><name sortKey="Hawkins, Mary E" sort="Hawkins, Mary E" uniqKey="Hawkins M" first="Mary E." last="Hawkins">Mary E. Hawkins</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Maryland</region>
</placeName>
<wicri:cityArea>Pediatric Oncology Branch, National Cancer Institute, 20892, Bethesda</wicri:cityArea>
</affiliation>
<affiliation></affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Cell Biochemistry and Biophysics</title>
<title level="j" type="abbrev">Cell Biochem Biophys</title>
<idno type="ISSN">1085-9195</idno>
<idno type="eISSN">1559-0283</idno>
<imprint><publisher>Humana Press</publisher>
<pubPlace>Totowa</pubPlace>
<date type="published" when="2001-04-01">2001-04-01</date>
<biblScope unit="volume">34</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="257">257</biblScope>
<biblScope unit="page" to="281">281</biblScope>
</imprint>
<idno type="ISSN">1085-9195</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1085-9195</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Pteridine nucleoside analog probes are highly fluorescent and offer different approaches to monitor subtle DNA interactions with other molecules. Similarities in structure and size to native nucleosides make it possible to incorporate these probes into oligonucleotides through the standard deoxyribose linkage. These probes are formulated as phosphoramidites and incorporated into oligonucleotides using automated DNA synthesis. Their position within the oligonucleotide renders them exquisitely sensitive to changes in structure as the oligonucleotide meets and reacts with other molecules. Changes are measured through fluorescence intensity, anisotropy, lifetimes, spectral shifts, and energy transfer. The fluorescence properties of pteridine nucleoside analogs as monomers and incorporated into single and double stranded oligonucleotides are reviewed. The two guanosine analogs, 3MI and 6MI, and two adenosine analogs, 6MAP and DMAP, are reviewed in detail along with applications utilizing them.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Maryland</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Maryland"><name sortKey="Hawkins, Mary E" sort="Hawkins, Mary E" uniqKey="Hawkins M" first="Mary E." last="Hawkins">Mary E. Hawkins</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003560 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003560 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:0ACDFD8F889CC4D53DC53B25F43DEA4B18CD03A4 |texte= Fluorescent pteridine nucleoside analogs }}
This area was generated with Dilib version V0.6.33. |