Gene silencing by double-stranded RNA
Identifieur interne : 003556 ( Main/Exploration ); précédent : 003555; suivant : 003557Gene silencing by double-stranded RNA
Auteurs : Richard W. Carthew [États-Unis]Source :
- Current Opinion in Cell Biology [ 0955-0674 ] ; 2001.
English descriptors
- KwdEn :
- Teeft :
- Antisense, Antisense strands, Biol, Caenorhabditis elegans, Carthew, Cleavage, Cosuppression, Drosophila, Dsrna, Dsrna endonuclease, Elegans, Foreign genes, Gene, Gene expression, Genome, Mrna, Mutant, Nuclease, Nucleotide, Nucleotide rnas, Pathway, Plant cell, Polymerase, Posttranscriptional gene, Proc natl acad, Ptgs, Rdrp, Rna, Rnai, Rnai signal, Specific sites, Transgene, Transposon, Transposon mobilization, Unpublished data, Virus resistance, Worm mutants.
Abstract
Abstract: Eukaryotes silence gene expression in the presence of double-stranded RNA homologous to the silenced gene. Silencing occurs by the targeted degradation of mRNA. Biochemical reactions that recapitulate this phenomenon generate RNA fragments of 21–23 nucleotides from the double-stranded RNA. These stably associate with an RNA endonuclease and probably serve as a discriminator to select mRNAs. Once selected, mRNAs are cleaved at sites 21–23 nucleotides apart. This mechanism, termed RNAi, has functional links to viral defense and silencing phenomena, such as cosuppression. It also functions to repress the hopping of transposable elements.
Url:
DOI: 10.1016/S0955-0674(00)00204-0
Affiliations:
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Le document en format XML
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Carthew</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260</wicri:regionArea><orgName type="university">Université de Pittsburgh</orgName><placeName><settlement type="city">Pittsburgh</settlement><region type="state">Pennsylvanie</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Current Opinion in Cell Biology</title><title level="j" type="abbrev">COCEBI</title><idno type="ISSN">0955-0674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">13</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="244">244</biblScope><biblScope unit="page" to="248">248</biblScope></imprint><idno type="ISSN">0955-0674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0955-0674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell biology</term><term>PTGS</term><term>RNAi</term><term>Techniques & Methods</term><term>dsRNA</term><term>gene silencing</term><term>mRNA cleavage</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Antisense</term><term>Antisense strands</term><term>Biol</term><term>Caenorhabditis elegans</term><term>Carthew</term><term>Cleavage</term><term>Cosuppression</term><term>Drosophila</term><term>Dsrna</term><term>Dsrna endonuclease</term><term>Elegans</term><term>Foreign genes</term><term>Gene</term><term>Gene expression</term><term>Genome</term><term>Mrna</term><term>Mutant</term><term>Nuclease</term><term>Nucleotide</term><term>Nucleotide rnas</term><term>Pathway</term><term>Plant cell</term><term>Polymerase</term><term>Posttranscriptional gene</term><term>Proc natl acad</term><term>Ptgs</term><term>Rdrp</term><term>Rna</term><term>Rnai</term><term>Rnai signal</term><term>Specific sites</term><term>Transgene</term><term>Transposon</term><term>Transposon mobilization</term><term>Unpublished data</term><term>Virus resistance</term><term>Worm mutants</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Eukaryotes silence gene expression in the presence of double-stranded RNA homologous to the silenced gene. Silencing occurs by the targeted degradation of mRNA. Biochemical reactions that recapitulate this phenomenon generate RNA fragments of 21–23 nucleotides from the double-stranded RNA. These stably associate with an RNA endonuclease and probably serve as a discriminator to select mRNAs. Once selected, mRNAs are cleaved at sites 21–23 nucleotides apart. This mechanism, termed RNAi, has functional links to viral defense and silencing phenomena, such as cosuppression. It also functions to repress the hopping of transposable elements.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>Pittsburgh</li></settlement><orgName><li>Université de Pittsburgh</li></orgName></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Carthew, Richard W" sort="Carthew, Richard W" uniqKey="Carthew R" first="Richard W" last="Carthew">Richard W. Carthew</name></region><name sortKey="Carthew, Richard W" sort="Carthew, Richard W" uniqKey="Carthew R" first="Richard W" last="Carthew">Richard W. Carthew</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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