Clustering of nucleobases with alkali metals studied by electrospray ionization tandem mass spectrometry: implications for mechanisms of multistrand DNA stabilization.
Identifieur interne : 003338 ( Main/Exploration ); précédent : 003337; suivant : 003339Clustering of nucleobases with alkali metals studied by electrospray ionization tandem mass spectrometry: implications for mechanisms of multistrand DNA stabilization.
Auteurs : Kim J. Koch [États-Unis] ; Tenna Aggerholm ; Sergio C. Nanita ; R Graham CooksSource :
- Journal of mass spectrometry : JMS [ 1076-5174 ] ; 2002.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : DNA, Metals, Alkaline Earth, Nucleosides, Nucleotides.
- Chromatography, High Pressure Liquid, Mass Spectrometry, Protons, Spectrometry, Mass, Electrospray Ionization.
Abstract
Self-clustering of the five common nucleobases was investigated by electrospray ionization tandem mass spectrometry and shown to provide insight into the non-covalent interactions between identical bases. Alkali and ammonium cations significantly increase self-aggregation of the nucleobases and lead to the formation of uniquely stable magic number clusters. Sodium adducts of guanine, thymine and uracil preferentially take the form of tetrameric (quartet) clusters. This gas-phase result correlates with previously reported solution-phase data on sodium cation stabilized guanosine, thymine and uracil quartet structures believed to be responsible for telomere stabilization. In the presence of potassium, cesium or ammonium cations, pentameric magic number clusters are formed from thymine and uracil, while in solution the nucleoside isoguanosine yields clusters of this favored size. The formation of magic number metaclusters occurs for thymine and uracil in the presence of ammonium cations. These doubly charged 10- and 15-mers are tentatively attributed to the formation of pentamer/ammonium cation/ pentamer sandwich structures.
DOI: 10.1002/jms.326
PubMed: 12125000
Affiliations:
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Koch</name><affiliation wicri:level="1"><nlm:affiliation>Purdue University, Department of Chemistry, West Lafayette, Indiana 47907, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Purdue University, Department of Chemistry, West Lafayette, Indiana 47907</wicri:regionArea><wicri:noRegion>Indiana 47907</wicri:noRegion></affiliation></author><author><name sortKey="Aggerholm, Tenna" sort="Aggerholm, Tenna" uniqKey="Aggerholm T" first="Tenna" last="Aggerholm">Tenna Aggerholm</name></author><author><name sortKey="Nanita, Sergio C" sort="Nanita, Sergio C" uniqKey="Nanita S" first="Sergio C" last="Nanita">Sergio C. Nanita</name></author><author><name sortKey="Cooks, R Graham" sort="Cooks, R Graham" uniqKey="Cooks R" first="R Graham" last="Cooks">R Graham Cooks</name></author></analytic><series><title level="j">Journal of mass spectrometry : JMS</title><idno type="ISSN">1076-5174</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>DNA (chemistry)</term><term>Mass Spectrometry</term><term>Metals, Alkaline Earth (chemistry)</term><term>Nucleosides (chemistry)</term><term>Nucleotides (chemistry)</term><term>Protons</term><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term><term>Chromatographie en phase liquide à haute performance</term><term>Métaux alcalinoterreux ()</term><term>Nucléosides ()</term><term>Nucléotides ()</term><term>Protons</term><term>Spectrométrie de masse</term><term>Spectrométrie de masse ESI</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA</term><term>Metals, Alkaline Earth</term><term>Nucleosides</term><term>Nucleotides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Mass Spectrometry</term><term>Protons</term><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>Chromatographie en phase liquide à haute performance</term><term>Métaux alcalinoterreux</term><term>Nucléosides</term><term>Nucléotides</term><term>Protons</term><term>Spectrométrie de masse</term><term>Spectrométrie de masse ESI</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Self-clustering of the five common nucleobases was investigated by electrospray ionization tandem mass spectrometry and shown to provide insight into the non-covalent interactions between identical bases. Alkali and ammonium cations significantly increase self-aggregation of the nucleobases and lead to the formation of uniquely stable magic number clusters. Sodium adducts of guanine, thymine and uracil preferentially take the form of tetrameric (quartet) clusters. This gas-phase result correlates with previously reported solution-phase data on sodium cation stabilized guanosine, thymine and uracil quartet structures believed to be responsible for telomere stabilization. In the presence of potassium, cesium or ammonium cations, pentameric magic number clusters are formed from thymine and uracil, while in solution the nucleoside isoguanosine yields clusters of this favored size. The formation of magic number metaclusters occurs for thymine and uracil in the presence of ammonium cations. These doubly charged 10- and 15-mers are tentatively attributed to the formation of pentamer/ammonium cation/ pentamer sandwich structures.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Aggerholm, Tenna" sort="Aggerholm, Tenna" uniqKey="Aggerholm T" first="Tenna" last="Aggerholm">Tenna Aggerholm</name><name sortKey="Cooks, R Graham" sort="Cooks, R Graham" uniqKey="Cooks R" first="R Graham" last="Cooks">R Graham Cooks</name><name sortKey="Nanita, Sergio C" sort="Nanita, Sergio C" uniqKey="Nanita S" first="Sergio C" last="Nanita">Sergio C. Nanita</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Koch, Kim J" sort="Koch, Kim J" uniqKey="Koch K" first="Kim J" last="Koch">Kim J. Koch</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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