Epstein–Barr Virus DNA is Detected in Peripheral Blood Mononuclear Cells of EBV-seronegative Infants with Infectious Mononucleosis-like Symptoms
Identifieur interne : 003280 ( Main/Exploration ); précédent : 003279; suivant : 003281Epstein–Barr Virus DNA is Detected in Peripheral Blood Mononuclear Cells of EBV-seronegative Infants with Infectious Mononucleosis-like Symptoms
Auteurs : Kazufumi Ikuta [Japon] ; Kyoko Saiga [Japon] ; Masanori Deguchi [Japon] ; Takeshi Sairenji [Japon]Source :
- Virus Genes [ 0920-8569 ] ; 2003-03-01.
English descriptors
Abstract
Abstract: We demonstrated Epstein–Barr virus (EBV) DNA in peripheral blood mononuclear cells (PBMCs) from infants with infectious mononucleosis- (IM) like symptoms. Thirteen of the 17 patients did not have EBV antibodies; however, EBV DNA was detected in 8 PBMC from the 13 seronegative patients by PCR. The 4 patients were retested in 6–12 months later. Three patients were still seronegative; however EBV DNA was not detected. One patient seroconverted and EBV DNA could still be detected. The transcript of EBNA1 was detected in one patient, but neither EBNA2 nor LMP2A were detected in all PBMC from the 4 tested patients. Type 1 EBV DNA was detected in 5 PBMC of 7 tested patients, and type 2 EBV DNA was detected in type 1 positive PBMC of one patient as well. The IL-1 β polymorphism that is reported to be one of the immunological factors of EBV seronegativity revealed no difference in IM-like patients. These results indicated that EBV infection occurs in EBV-seronegative IM-like infants; however, the modes of infection are clearly different from IM.
Url:
DOI: 10.1023/A:1023487413912
Affiliations:
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Thirteen of the 17 patients did not have EBV antibodies; however, EBV DNA was detected in 8 PBMC from the 13 seronegative patients by PCR. The 4 patients were retested in 6–12 months later. Three patients were still seronegative; however EBV DNA was not detected. One patient seroconverted and EBV DNA could still be detected. The transcript of EBNA1 was detected in one patient, but neither EBNA2 nor LMP2A were detected in all PBMC from the 4 tested patients. Type 1 EBV DNA was detected in 5 PBMC of 7 tested patients, and type 2 EBV DNA was detected in type 1 positive PBMC of one patient as well. The IL-1 β polymorphism that is reported to be one of the immunological factors of EBV seronegativity revealed no difference in IM-like patients. 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