Analysis of the Frequencies and of the Memory T Cell Phenotypes of Human CD8+ T Cells Specific for Influenza A Viruses
Identifieur interne : 003249 ( Main/Exploration ); précédent : 003248; suivant : 003250Analysis of the Frequencies and of the Memory T Cell Phenotypes of Human CD8+ T Cells Specific for Influenza A Viruses
Auteurs : Xiao-Song He ; Kutubuddin Mahmood ; Holden T. Maecker [États-Unis] ; Tyson H. Holmes ; George W. Kemble ; Ann M. Arvin ; Harry B. GreenbergSource :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2003.
Abstract
We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry–based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I–restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus–specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I–restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus– and cytomegalovirus-specific CD8+ T cells suggest that influenza virus–specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis
Url:
DOI: 10.1086/368218
Affiliations:
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