Assessing unmodified 70-mer oligonucleotide probe performance on glass-slide microarrays
Identifieur interne : 003232 ( Main/Exploration ); précédent : 003231; suivant : 003233Assessing unmodified 70-mer oligonucleotide probe performance on glass-slide microarrays
Auteurs : Hong-Ying Wang [États-Unis] ; Renae L. Malek [États-Unis] ; Anne E. Kwitek [États-Unis] ; Andrew S. Greene [États-Unis] ; Truong V. Luu [États-Unis] ; Babak Behbahani [États-Unis] ; Bryan Frank [États-Unis] ; John Quackenbush [États-Unis] ; Norman H. Lee [États-Unis]Source :
- Genome Biology [ 1465-6906 ] ; 2003.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Arabidopsis (génétique), Réaction de polymérisation en chaîne (), Réaction de polymérisation en chaîne (normes), Sensibilité et spécificité, Sondes oligonucléotidiques (génétique), Séquençage par oligonucléotides en batterie (), Séquençage par oligonucléotides en batterie (normes).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Complementary, Oligonucleotide Probes.
- genetics : Arabidopsis.
- methods : Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction.
- standards : Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction.
- Sensitivity and Specificity.
Abstract
Long oligonucleotide microarrays are potentially more cost- and management efficient than cDNA microarrays. Unmodified sense and antisense 70-mer oligonucleotides were synthesized and compared with PCR-amplified cDNA clones corresponding to the same genes. The correlation coefficient between oligonucleotide and cDNA probes for identifying differentially expressed genes was 0.80.
Url:
PubMed: 12540297
PubMed Central: 151289
Affiliations:
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Kwitek</name><affiliation wicri:level="2"><nlm:aff id="I2">Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226</wicri:regionArea><placeName><region type="state">Wisconsin</region></placeName></affiliation></author><author><name sortKey="Greene, Andrew S" sort="Greene, Andrew S" uniqKey="Greene A" first="Andrew S" last="Greene">Andrew S. Greene</name><affiliation wicri:level="2"><nlm:aff id="I2">Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226</wicri:regionArea><placeName><region type="state">Wisconsin</region></placeName></affiliation></author><author><name sortKey="Luu, Truong V" sort="Luu, Truong V" uniqKey="Luu T" first="Truong V" last="Luu">Truong V. Luu</name><affiliation wicri:level="2"><nlm:aff id="I1">The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Behbahani, Babak" sort="Behbahani, Babak" uniqKey="Behbahani B" first="Babak" last="Behbahani">Babak Behbahani</name><affiliation wicri:level="2"><nlm:aff id="I1">The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Frank, Bryan" sort="Frank, Bryan" uniqKey="Frank B" first="Bryan" last="Frank">Bryan Frank</name><affiliation wicri:level="2"><nlm:aff id="I1">The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Quackenbush, John" sort="Quackenbush, John" uniqKey="Quackenbush J" first="John" last="Quackenbush">John Quackenbush</name><affiliation wicri:level="2"><nlm:aff id="I1">The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Lee, Norman H" sort="Lee, Norman H" uniqKey="Lee N" first="Norman H" last="Lee">Norman H. Lee</name><affiliation wicri:level="2"><nlm:aff id="I1">The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="I3">Department of Pharmacology, The George Washington University Medical Center, 2300 Eye Street NW, Washington, DC 20037, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology, The George Washington University Medical Center, 2300 Eye Street NW, Washington, DC 20037</wicri:regionArea><placeName><region type="state">District de Columbia</region></placeName></affiliation></author></analytic><series><title level="j">Genome Biology</title><idno type="ISSN">1465-6906</idno><idno type="eISSN">1465-6914</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (genetics)</term><term>DNA, Complementary (genetics)</term><term>Oligonucleotide Array Sequence Analysis (methods)</term><term>Oligonucleotide Array Sequence Analysis (standards)</term><term>Oligonucleotide Probes (genetics)</term><term>Polymerase Chain Reaction (methods)</term><term>Polymerase Chain Reaction (standards)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>Arabidopsis (génétique)</term><term>Réaction de polymérisation en chaîne ()</term><term>Réaction de polymérisation en chaîne (normes)</term><term>Sensibilité et spécificité</term><term>Sondes oligonucléotidiques (génétique)</term><term>Séquençage par oligonucléotides en batterie ()</term><term>Séquençage par oligonucléotides en batterie (normes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Oligonucleotide Probes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Arabidopsis</term><term>Sondes oligonucléotidiques</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Oligonucleotide Array Sequence Analysis</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Réaction de polymérisation en chaîne</term><term>Séquençage par oligonucléotides en batterie</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Oligonucleotide Array Sequence Analysis</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Réaction de polymérisation en chaîne</term><term>Sensibilité et spécificité</term><term>Séquençage par oligonucléotides en batterie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Long oligonucleotide microarrays are potentially more cost- and management efficient than cDNA microarrays. Unmodified sense and antisense 70-mer oligonucleotides were synthesized and compared with PCR-amplified cDNA clones corresponding to the same genes. The correlation coefficient between oligonucleotide and cDNA probes for identifying differentially expressed genes was 0.80.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>District de Columbia</li><li>Maryland</li><li>Wisconsin</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Wang, Hong Ying" sort="Wang, Hong Ying" uniqKey="Wang H" first="Hong-Ying" last="Wang">Hong-Ying Wang</name></region><name sortKey="Behbahani, Babak" sort="Behbahani, Babak" uniqKey="Behbahani B" first="Babak" last="Behbahani">Babak Behbahani</name><name sortKey="Frank, Bryan" sort="Frank, Bryan" uniqKey="Frank B" first="Bryan" last="Frank">Bryan Frank</name><name sortKey="Greene, Andrew S" sort="Greene, Andrew S" uniqKey="Greene A" first="Andrew S" last="Greene">Andrew S. Greene</name><name sortKey="Kwitek, Anne E" sort="Kwitek, Anne E" uniqKey="Kwitek A" first="Anne E" last="Kwitek">Anne E. Kwitek</name><name sortKey="Lee, Norman H" sort="Lee, Norman H" uniqKey="Lee N" first="Norman H" last="Lee">Norman H. Lee</name><name sortKey="Lee, Norman H" sort="Lee, Norman H" uniqKey="Lee N" first="Norman H" last="Lee">Norman H. Lee</name><name sortKey="Luu, Truong V" sort="Luu, Truong V" uniqKey="Luu T" first="Truong V" last="Luu">Truong V. Luu</name><name sortKey="Malek, Renae L" sort="Malek, Renae L" uniqKey="Malek R" first="Renae L" last="Malek">Renae L. Malek</name><name sortKey="Quackenbush, John" sort="Quackenbush, John" uniqKey="Quackenbush J" first="John" last="Quackenbush">John Quackenbush</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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