Epitope mapping and evaluation of specificity of T-helper sites in four major antigenic peptides of chicken riboflavin carrier protein in outbred rats.
Identifieur interne : 003221 ( Main/Exploration ); précédent : 003220; suivant : 003222Epitope mapping and evaluation of specificity of T-helper sites in four major antigenic peptides of chicken riboflavin carrier protein in outbred rats.
Auteurs : Sarada Subramanian [Inde] ; S. Andal ; Anjali A. Karande ; P. Radhakantha AdigaSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2003.
Descripteurs français
- KwdFr :
- Analyse de séquence de protéine (), Animaux, Cartographie épitopique (), Cellules cultivées, Données de séquences moléculaires, Déterminants antigéniques des lymphocytes T (), Déterminants antigéniques des lymphocytes T (immunologie), Lymphocytes T auxiliaires (), Lymphocytes T auxiliaires (immunologie), Peptides (), Peptides (immunologie), Poulets, Protéines de transport (), Protéines de transport (immunologie), Protéines de transport membranaire, Rat Wistar, Rats, Sensibilité et spécificité, Séquence d'acides aminés, Vaccins contraceptifs (), Vaccins contraceptifs (immunologie), Épitopes ().
- MESH :
- immunologie : Déterminants antigéniques des lymphocytes T, Lymphocytes T auxiliaires, Peptides, Protéines de transport, Vaccins contraceptifs.
- Analyse de séquence de protéine, Animaux, Cartographie épitopique, Cellules cultivées, Données de séquences moléculaires, Déterminants antigéniques des lymphocytes T, Lymphocytes T auxiliaires, Peptides, Poulets, Protéines de transport, Protéines de transport membranaire, Rat Wistar, Rats, Sensibilité et spécificité, Séquence d'acides aminés, Vaccins contraceptifs, Épitopes.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Carrier Proteins (chemistry), Carrier Proteins (immunology), Cells, Cultured, Chickens, Epitope Mapping (methods), Epitopes (chemistry), Epitopes, T-Lymphocyte (chemistry), Epitopes, T-Lymphocyte (immunology), Membrane Transport Proteins, Molecular Sequence Data, Peptides (chemistry), Peptides (classification), Peptides (immunology), Rats, Rats, Wistar, Sensitivity and Specificity, Sequence Analysis, Protein (methods), T-Lymphocytes, Helper-Inducer (chemistry), T-Lymphocytes, Helper-Inducer (immunology), Vaccines, Contraceptive (chemistry), Vaccines, Contraceptive (immunology).
- MESH :
- chemical , chemistry : Carrier Proteins, Epitopes, Epitopes, T-Lymphocyte, Peptides, Vaccines, Contraceptive.
- chemical , classification : Peptides.
- chemical , immunology : Carrier Proteins, Epitopes, T-Lymphocyte, Peptides, Vaccines, Contraceptive.
- chemistry : T-Lymphocytes, Helper-Inducer.
- immunology : T-Lymphocytes, Helper-Inducer.
- methods : Epitope Mapping, Sequence Analysis, Protein.
- Amino Acid Sequence, Animals, Cells, Cultured, Chickens, Membrane Transport Proteins, Molecular Sequence Data, Rats, Rats, Wistar, Sensitivity and Specificity.
Abstract
This paper reviews our studies on synthetic peptides spanning the major antigenic determinants of the chicken riboflavin carrier protein (RCP; 219 AA). These determinants are composed of residues 4-24 (YGC), 64-83 (CED), 130-147 (GEN), and 200-219 (HAC) and function as minivaccines in terms of eliciting anti-peptide antibodies which recognize the native protein and are particularly promising contraceptive vaccine candidates. We have used 15-residue synthetic peptides to define short sequences involved in interaction with antibody and with T-cells. We have mapped the boundaries of T-cell epitopes of these peptides in outbred rats by immunizing the animals with each peptide and assaying the popliteal lymph node cell proliferation against a series of overlapping synthetic 15-mers covering the entire length of the individual peptides. The peptides YGC, GEN, and HAC harboured a single T-cell epitope each whereas the peptide CED exhibited bimodal response possessing two epitopes, one at N-terminus and the other at the C-terminus. These studies provide insight into the way in which an immunogen is viewed by the immune system. In addition, preferential T-cell helper function for B cells recognizing unique determinants on the same molecule was demonstrated. This information helps in exploiting synthetic peptides in the construction of designer immunogens which have potential as candidate vaccines.
DOI: 10.1016/j.bbrc.2003.09.162
PubMed: 14575688
Affiliations:
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Le document en format XML
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Andal</name></author><author><name sortKey="Karande, Anjali A" sort="Karande, Anjali A" uniqKey="Karande A" first="Anjali A" last="Karande">Anjali A. Karande</name></author><author><name sortKey="Radhakantha Adiga, P" sort="Radhakantha Adiga, P" uniqKey="Radhakantha Adiga P" first="P" last="Radhakantha Adiga">P. Radhakantha Adiga</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Carrier Proteins (chemistry)</term><term>Carrier Proteins (immunology)</term><term>Cells, Cultured</term><term>Chickens</term><term>Epitope Mapping (methods)</term><term>Epitopes (chemistry)</term><term>Epitopes, T-Lymphocyte (chemistry)</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>Membrane Transport Proteins</term><term>Molecular Sequence Data</term><term>Peptides (chemistry)</term><term>Peptides (classification)</term><term>Peptides (immunology)</term><term>Rats</term><term>Rats, Wistar</term><term>Sensitivity and Specificity</term><term>Sequence Analysis, Protein (methods)</term><term>T-Lymphocytes, Helper-Inducer (chemistry)</term><term>T-Lymphocytes, Helper-Inducer (immunology)</term><term>Vaccines, Contraceptive (chemistry)</term><term>Vaccines, Contraceptive (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence de protéine ()</term><term>Animaux</term><term>Cartographie épitopique ()</term><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Déterminants antigéniques des lymphocytes T ()</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Lymphocytes T auxiliaires ()</term><term>Lymphocytes T auxiliaires (immunologie)</term><term>Peptides ()</term><term>Peptides (immunologie)</term><term>Poulets</term><term>Protéines de transport ()</term><term>Protéines de transport (immunologie)</term><term>Protéines de transport membranaire</term><term>Rat Wistar</term><term>Rats</term><term>Sensibilité et spécificité</term><term>Séquence d'acides aminés</term><term>Vaccins contraceptifs ()</term><term>Vaccins contraceptifs (immunologie)</term><term>Épitopes ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Carrier Proteins</term><term>Epitopes</term><term>Epitopes, T-Lymphocyte</term><term>Peptides</term><term>Vaccines, Contraceptive</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Carrier Proteins</term><term>Epitopes, T-Lymphocyte</term><term>Peptides</term><term>Vaccines, Contraceptive</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>T-Lymphocytes, Helper-Inducer</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Déterminants antigéniques des lymphocytes T</term><term>Lymphocytes T auxiliaires</term><term>Peptides</term><term>Protéines de transport</term><term>Vaccins contraceptifs</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>T-Lymphocytes, Helper-Inducer</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Epitope Mapping</term><term>Sequence Analysis, Protein</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cells, Cultured</term><term>Chickens</term><term>Membrane Transport Proteins</term><term>Molecular Sequence Data</term><term>Rats</term><term>Rats, Wistar</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence de protéine</term><term>Animaux</term><term>Cartographie épitopique</term><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Déterminants antigéniques des lymphocytes T</term><term>Lymphocytes T auxiliaires</term><term>Peptides</term><term>Poulets</term><term>Protéines de transport</term><term>Protéines de transport membranaire</term><term>Rat Wistar</term><term>Rats</term><term>Sensibilité et spécificité</term><term>Séquence d'acides aminés</term><term>Vaccins contraceptifs</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This paper reviews our studies on synthetic peptides spanning the major antigenic determinants of the chicken riboflavin carrier protein (RCP; 219 AA). These determinants are composed of residues 4-24 (YGC), 64-83 (CED), 130-147 (GEN), and 200-219 (HAC) and function as minivaccines in terms of eliciting anti-peptide antibodies which recognize the native protein and are particularly promising contraceptive vaccine candidates. We have used 15-residue synthetic peptides to define short sequences involved in interaction with antibody and with T-cells. We have mapped the boundaries of T-cell epitopes of these peptides in outbred rats by immunizing the animals with each peptide and assaying the popliteal lymph node cell proliferation against a series of overlapping synthetic 15-mers covering the entire length of the individual peptides. The peptides YGC, GEN, and HAC harboured a single T-cell epitope each whereas the peptide CED exhibited bimodal response possessing two epitopes, one at N-terminus and the other at the C-terminus. These studies provide insight into the way in which an immunogen is viewed by the immune system. In addition, preferential T-cell helper function for B cells recognizing unique determinants on the same molecule was demonstrated. This information helps in exploiting synthetic peptides in the construction of designer immunogens which have potential as candidate vaccines.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Andal, S" sort="Andal, S" uniqKey="Andal S" first="S" last="Andal">S. Andal</name><name sortKey="Karande, Anjali A" sort="Karande, Anjali A" uniqKey="Karande A" first="Anjali A" last="Karande">Anjali A. Karande</name><name sortKey="Radhakantha Adiga, P" sort="Radhakantha Adiga, P" uniqKey="Radhakantha Adiga P" first="P" last="Radhakantha Adiga">P. Radhakantha Adiga</name></noCountry><country name="Inde"><noRegion><name sortKey="Subramanian, Sarada" sort="Subramanian, Sarada" uniqKey="Subramanian S" first="Sarada" last="Subramanian">Sarada Subramanian</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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