Fluorescent Polymeric Transducer for the Rapid, Simple, and Specific Detection of Nucleic Acids at the Zeptomole Level
Identifieur interne : 003091 ( Main/Exploration ); précédent : 003090; suivant : 003092Fluorescent Polymeric Transducer for the Rapid, Simple, and Specific Detection of Nucleic Acids at the Zeptomole Level
Auteurs : Kim Doré [Canada] ; Sébastien Dubus [Canada] ; Hoang-Anh Ho [Canada] ; Isabelle Lévesque [Canada] ; Maryse Brunette [Canada] ; Geneviève Corbeil [Canada] ; Maurice Boissinot [Canada] ; Guy Boivin [Canada] ; Michel G. Bergeron [Canada] ; Denis Boudreau [Canada] ; Mario Leclerc [Canada]Source :
- Journal of the American Chemical Society [ 0002-7863 ] ; 2004.
Abstract
We report the specific detection of a few hundred molecules of genetic material using a fluorescent polythiophene biosensor. Such recognition is based on simple electrostatic interactions between a cationic polymeric optical transducer and the negatively charged nucleic acid target and can be done in less than 1 h, simply and affordably, and without any chemical reaction. This simple system is versatile enough to detect nucleic acids of various lengths, including a segment from the RNA genome of the Influenza virus.
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DOI: 10.1021/ja038900d
Affiliations:
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des Macromoléculesand Centre d'Optique, Photonique et Laser, Département de Chimie, Université Laval,Quebec City, Quebec, Canada G1K 7P4, and Centre de Recherche en Infectiologie,Centre Hospitalier Universitaire de Québec, Pavillon CHUL, Université Laval,Quebec City, Quebec</wicri:regionArea><wicri:noRegion>Quebec</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Ho, Hoang Anh" sort="Ho, Hoang Anh" uniqKey="Ho H" first="Hoang-Anh" last="Ho">Hoang-Anh Ho</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Contribution from the Centre de Recherche en Sciences et Ingénierie des Macromoléculesand Centre d'Optique, Photonique et Laser, Département de Chimie, Université Laval,Quebec City, Quebec, Canada G1K 7P4, and Centre de Recherche en Infectiologie,Centre Hospitalier Universitaire de Québec, Pavillon CHUL, Université Laval,Quebec City, Quebec</wicri:regionArea><wicri:noRegion>Quebec</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Levesque, Isabelle" sort="Levesque, Isabelle" uniqKey="Levesque I" first="Isabelle" last="Lévesque">Isabelle Lévesque</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Contribution from the Centre de Recherche en Sciences et Ingénierie des Macromoléculesand Centre d'Optique, Photonique et Laser, Département de Chimie, Université Laval,Quebec City, Quebec, Canada G1K 7P4, and Centre de Recherche en Infectiologie,Centre Hospitalier Universitaire de Québec, Pavillon CHUL, Université Laval,Quebec City, Quebec</wicri:regionArea><wicri:noRegion>Quebec</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Brunette, Maryse" sort="Brunette, Maryse" uniqKey="Brunette M" first="Maryse" last="Brunette">Maryse Brunette</name><affiliation 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Boivin</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Contribution from the Centre de Recherche en Sciences et Ingénierie des Macromoléculesand Centre d'Optique, Photonique et Laser, Département de Chimie, Université Laval,Quebec City, Quebec, Canada G1K 7P4, and Centre de Recherche en Infectiologie,Centre Hospitalier Universitaire de Québec, Pavillon CHUL, Université Laval,Quebec City, Quebec</wicri:regionArea><wicri:noRegion>Quebec</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Bergeron, Michel G" sort="Bergeron, Michel G" uniqKey="Bergeron M" first="Michel G." last="Bergeron">Michel G. Bergeron</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Contribution from the Centre de Recherche en Sciences et Ingénierie des Macromoléculesand Centre d'Optique, Photonique et Laser, Département de Chimie, Université Laval,Quebec City, Quebec, Canada G1K 7P4, and Centre de Recherche en Infectiologie,Centre Hospitalier Universitaire de Québec, Pavillon CHUL, Université Laval,Quebec City, Quebec</wicri:regionArea><wicri:noRegion>Quebec</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Boudreau, Denis" sort="Boudreau, Denis" uniqKey="Boudreau D" first="Denis" last="Boudreau">Denis Boudreau</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Contribution from the Centre de Recherche en Sciences et Ingénierie des Macromoléculesand Centre d'Optique, Photonique et Laser, Département de Chimie, Université Laval,Quebec City, Quebec, Canada G1K 7P4, and Centre de Recherche en Infectiologie,Centre Hospitalier Universitaire de Québec, Pavillon CHUL, Université Laval,Quebec City, Quebec</wicri:regionArea><wicri:noRegion>Quebec</wicri:noRegion></affiliation><affiliation></affiliation><affiliation></affiliation></author><author><name sortKey="Leclerc, Mario" sort="Leclerc, Mario" uniqKey="Leclerc M" first="Mario" last="Leclerc">Mario Leclerc</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Contribution from the Centre de Recherche en Sciences et Ingénierie des Macromoléculesand Centre d'Optique, Photonique et Laser, Département de Chimie, Université Laval,Quebec City, Quebec, Canada G1K 7P4, and Centre de Recherche en Infectiologie,Centre Hospitalier Universitaire de Québec, Pavillon CHUL, Université Laval,Quebec City, Quebec</wicri:regionArea><wicri:noRegion>Quebec</wicri:noRegion></affiliation><affiliation></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of the American Chemical Society</title><title level="j" type="abbrev">J. Am. Chem. Soc.</title><idno type="ISSN">0002-7863</idno><idno type="eISSN">1520-5126</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published">2004</date><date type="published">2004</date><biblScope unit="vol">126</biblScope><biblScope unit="issue">13</biblScope><biblScope unit="page" from="4240">4240</biblScope><biblScope unit="page" to="4244">4244</biblScope></imprint><idno type="ISSN">0002-7863</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0002-7863</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">We report the specific detection of a few hundred molecules of genetic material using a fluorescent polythiophene biosensor. Such recognition is based on simple electrostatic interactions between a cationic polymeric optical transducer and the negatively charged nucleic acid target and can be done in less than 1 h, simply and affordably, and without any chemical reaction. This simple system is versatile enough to detect nucleic acids of various lengths, including a segment from the RNA genome of the Influenza virus.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Dore, Kim" sort="Dore, Kim" uniqKey="Dore K" first="Kim" last="Doré">Kim Doré</name></noRegion><name sortKey="Bergeron, Michel G" sort="Bergeron, Michel G" uniqKey="Bergeron M" first="Michel G." last="Bergeron">Michel G. Bergeron</name><name sortKey="Boissinot, Maurice" sort="Boissinot, Maurice" uniqKey="Boissinot M" first="Maurice" last="Boissinot">Maurice Boissinot</name><name sortKey="Boivin, Guy" sort="Boivin, Guy" uniqKey="Boivin G" first="Guy" last="Boivin">Guy Boivin</name><name sortKey="Boudreau, Denis" sort="Boudreau, Denis" uniqKey="Boudreau D" first="Denis" last="Boudreau">Denis Boudreau</name><name sortKey="Brunette, Maryse" sort="Brunette, Maryse" uniqKey="Brunette M" first="Maryse" last="Brunette">Maryse Brunette</name><name sortKey="Corbeil, Genevieve" sort="Corbeil, Genevieve" uniqKey="Corbeil G" first="Geneviève" last="Corbeil">Geneviève Corbeil</name><name sortKey="Dubus, Sebastien" sort="Dubus, Sebastien" uniqKey="Dubus S" first="Sébastien" last="Dubus">Sébastien Dubus</name><name sortKey="Ho, Hoang Anh" sort="Ho, Hoang Anh" uniqKey="Ho H" first="Hoang-Anh" last="Ho">Hoang-Anh Ho</name><name sortKey="Leclerc, Mario" sort="Leclerc, Mario" uniqKey="Leclerc M" first="Mario" last="Leclerc">Mario Leclerc</name><name sortKey="Levesque, Isabelle" sort="Levesque, Isabelle" uniqKey="Levesque I" first="Isabelle" last="Lévesque">Isabelle Lévesque</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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