Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry
Identifieur interne : 003020 ( Main/Exploration ); précédent : 003019; suivant : 003021Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry
Auteurs : An V. Willems ; Dieter L. Deforce ; Carlos H. Van Peteghem [Belgique] ; Jan F. Van Bocxlaer [Belgique]Source :
- ELECTROPHORESIS [ 0173-0835 ] ; 2005-04.
English descriptors
- Teeft :
- Aaaf, Abacavir, Acetate, Acetic, Acetic acid, Acetonitrile, Acidic, Adduct, Adduct samples, Adducted, Adducted nucleotides, Adenine, Adenosine, Alkylation, Ammonium, Ammonium acetate, Ammonium carbonate, Ammonium carbonate buffer, Ammonium ions, Anal, Analogue, Analyte, Analytes, Analytical perspectives, Bapde, Baseline separation, Bgcde, Borate, Boric acid, Buffer system, Cahours, Capillary, Capillary isotachophoresis, Caproic acid, Carbonate, Carbovir, Carcinogen, Cationic, Cell extracts, Chem, Chloroform, Chromatogr, Citp, Coaxial, Coaxial sheath, Commun, Constituent, Cytidine, Deforce, Detection limit, Dgmp, Digestion, Dihydrodiol epoxide, Dinucleotide, Diol epoxide, Dippthr, Electrokinetically, Electrolyte, Electrophoresis, Electrophoretic, Electrospray, Epoxide, Esmans, Ethanol, Ethanol precipitation, Ether, Flow rate, Fmol, Formic, Formic acid, Gmbh, Guanosine, Hplc, Hydrodynamic injection, Hydrodynamically, Interface, Ionic strength, Ionization, Isopropanol, Isotachophoresis, Kgaa, Liquid junction interface, Long acetate, Mass spectrom, Mass spectrometer, Mass spectrum, Matrix, Mbar, Meoh, Methanol, Migration time, Mobile phase, Mode concentration, Moiety, Molecular mass, Monophosphate, Natural nucleosides, Nicotinamide, Nuclease, Nucleic, Nucleic acid constituents, Nucleic acids, Nucleoside, Nucleoside analogues, Nucleotide, Oligonucleotide, Oligonucleotides, Other hand, Other interfaces, Oxide, Phenyl glycidyl ether, Phosphate group, Phosphodiesterase, Phosphorylated, Phosphorylated metabolites, Polybrene, Polycyclic, Polymer, Precipitation, Preconcentration, Quadrupole, Quadrupole water, Quality control, Rapid commun, Reaction products, Sample preparation, Selectivity, Separation efficiency, Sheath, Sheathless, Sheathless interface, Solute, Spectrom, Spectrometry, Styrene oxide, Tandem, Thymidine, Trap acetate, Triphosphate, Triple quadrupole, Uncoated, Unmodified, Unmodified nucleotides, Uridine, Verlag, Verlag gmbh, Vouros, Weinheim, Weinheim analysis, Weinheim electrophoresis, Willems.
Abstract
This review is focused on the capillary electrophoresis‐mass spectrometric (CE‐MS) analysis of nucleic acid constituents in the broadest sense, going from nucleotides and adducted nucleotides over nucleoside analogues to oligonucleotides. These nucleic acid constituents play an important role in a variety of biochemical processes. Hence, their isolation, identification, and quantification will undoubtedly help reveal the process of life and disease mechanisms, such as carcinogenesis, and can also be useful for antitumor and antiviral drug research to provide valuable information about mechanism of action, pharmacokinetics, pharmacodynamics, toxicity, therapeutic drug level monitoring, and quality control related to this substance class. Fundamental investigations into their structure, the search for modifications, the occurrence and biochemical impact of structural variation amongst others, are therefore of great value. In view of the related bioanalytical procedures, the coupling of CE to MS has emerged as a powerful tool for the analysis of the complex mixtures of nucleic acid constituents: CE confers rapid analysis and efficient resolution, while MS provides high selectivity and sensitivity with structural characterization of minute amounts of compound. After an introduction about the biochemical and analytical perspectives on the nucleic acid constituents, the different modes of CE used in this field of research as well as the relevant CE‐MS interfaces and the difficulties associated with quantitative CE‐MS are briefly discussed. A large section is finally devoted to field‐oriented applications.
Url:
DOI: 10.1002/elps.200410278
Affiliations:
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Willems</name><affiliation><wicri:noCountry code="no comma">Laboratory of Medical Biochemistry & Clinical Analysis</wicri:noCountry></affiliation></author><author><name sortKey="Deforce, Dieter L" sort="Deforce, Dieter L" uniqKey="Deforce D" first="Dieter L." last="Deforce">Dieter L. Deforce</name><affiliation><wicri:noCountry code="no comma">Laboratory of Pharmaceutical Biotechnology</wicri:noCountry></affiliation></author><author><name sortKey="Van Peteghem, Carlos H" sort="Van Peteghem, Carlos H" uniqKey="Van Peteghem C" first="Carlos H." last="Van Peteghem">Carlos H. 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when="2005-04">2005-04</date></imprint><idno type="ISSN">0173-0835</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0173-0835</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aaaf</term><term>Abacavir</term><term>Acetate</term><term>Acetic</term><term>Acetic acid</term><term>Acetonitrile</term><term>Acidic</term><term>Adduct</term><term>Adduct samples</term><term>Adducted</term><term>Adducted nucleotides</term><term>Adenine</term><term>Adenosine</term><term>Alkylation</term><term>Ammonium</term><term>Ammonium acetate</term><term>Ammonium carbonate</term><term>Ammonium carbonate buffer</term><term>Ammonium ions</term><term>Anal</term><term>Analogue</term><term>Analyte</term><term>Analytes</term><term>Analytical perspectives</term><term>Bapde</term><term>Baseline separation</term><term>Bgcde</term><term>Borate</term><term>Boric acid</term><term>Buffer system</term><term>Cahours</term><term>Capillary</term><term>Capillary isotachophoresis</term><term>Caproic acid</term><term>Carbonate</term><term>Carbovir</term><term>Carcinogen</term><term>Cationic</term><term>Cell extracts</term><term>Chem</term><term>Chloroform</term><term>Chromatogr</term><term>Citp</term><term>Coaxial</term><term>Coaxial sheath</term><term>Commun</term><term>Constituent</term><term>Cytidine</term><term>Deforce</term><term>Detection limit</term><term>Dgmp</term><term>Digestion</term><term>Dihydrodiol epoxide</term><term>Dinucleotide</term><term>Diol epoxide</term><term>Dippthr</term><term>Electrokinetically</term><term>Electrolyte</term><term>Electrophoresis</term><term>Electrophoretic</term><term>Electrospray</term><term>Epoxide</term><term>Esmans</term><term>Ethanol</term><term>Ethanol precipitation</term><term>Ether</term><term>Flow rate</term><term>Fmol</term><term>Formic</term><term>Formic acid</term><term>Gmbh</term><term>Guanosine</term><term>Hplc</term><term>Hydrodynamic injection</term><term>Hydrodynamically</term><term>Interface</term><term>Ionic strength</term><term>Ionization</term><term>Isopropanol</term><term>Isotachophoresis</term><term>Kgaa</term><term>Liquid junction interface</term><term>Long acetate</term><term>Mass spectrom</term><term>Mass spectrometer</term><term>Mass spectrum</term><term>Matrix</term><term>Mbar</term><term>Meoh</term><term>Methanol</term><term>Migration time</term><term>Mobile phase</term><term>Mode concentration</term><term>Moiety</term><term>Molecular mass</term><term>Monophosphate</term><term>Natural nucleosides</term><term>Nicotinamide</term><term>Nuclease</term><term>Nucleic</term><term>Nucleic acid constituents</term><term>Nucleic acids</term><term>Nucleoside</term><term>Nucleoside analogues</term><term>Nucleotide</term><term>Oligonucleotide</term><term>Oligonucleotides</term><term>Other hand</term><term>Other interfaces</term><term>Oxide</term><term>Phenyl glycidyl ether</term><term>Phosphate group</term><term>Phosphodiesterase</term><term>Phosphorylated</term><term>Phosphorylated metabolites</term><term>Polybrene</term><term>Polycyclic</term><term>Polymer</term><term>Precipitation</term><term>Preconcentration</term><term>Quadrupole</term><term>Quadrupole water</term><term>Quality control</term><term>Rapid commun</term><term>Reaction products</term><term>Sample preparation</term><term>Selectivity</term><term>Separation efficiency</term><term>Sheath</term><term>Sheathless</term><term>Sheathless interface</term><term>Solute</term><term>Spectrom</term><term>Spectrometry</term><term>Styrene oxide</term><term>Tandem</term><term>Thymidine</term><term>Trap acetate</term><term>Triphosphate</term><term>Triple quadrupole</term><term>Uncoated</term><term>Unmodified</term><term>Unmodified nucleotides</term><term>Uridine</term><term>Verlag</term><term>Verlag gmbh</term><term>Vouros</term><term>Weinheim</term><term>Weinheim analysis</term><term>Weinheim electrophoresis</term><term>Willems</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This review is focused on the capillary electrophoresis‐mass spectrometric (CE‐MS) analysis of nucleic acid constituents in the broadest sense, going from nucleotides and adducted nucleotides over nucleoside analogues to oligonucleotides. These nucleic acid constituents play an important role in a variety of biochemical processes. Hence, their isolation, identification, and quantification will undoubtedly help reveal the process of life and disease mechanisms, such as carcinogenesis, and can also be useful for antitumor and antiviral drug research to provide valuable information about mechanism of action, pharmacokinetics, pharmacodynamics, toxicity, therapeutic drug level monitoring, and quality control related to this substance class. Fundamental investigations into their structure, the search for modifications, the occurrence and biochemical impact of structural variation amongst others, are therefore of great value. In view of the related bioanalytical procedures, the coupling of CE to MS has emerged as a powerful tool for the analysis of the complex mixtures of nucleic acid constituents: CE confers rapid analysis and efficient resolution, while MS provides high selectivity and sensitivity with structural characterization of minute amounts of compound. After an introduction about the biochemical and analytical perspectives on the nucleic acid constituents, the different modes of CE used in this field of research as well as the relevant CE‐MS interfaces and the difficulties associated with quantitative CE‐MS are briefly discussed. A large section is finally devoted to field‐oriented applications.</div></front></TEI><affiliations><list><country><li>Belgique</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><noCountry><name sortKey="Deforce, Dieter L" sort="Deforce, Dieter L" uniqKey="Deforce D" first="Dieter L." last="Deforce">Dieter L. Deforce</name><name sortKey="Willems, An V" sort="Willems, An V" uniqKey="Willems A" first="An V." last="Willems">An V. Willems</name></noCountry><country name="Belgique"><region name="Région flamande"><name sortKey="Van Peteghem, Carlos H" sort="Van Peteghem, Carlos H" uniqKey="Van Peteghem C" first="Carlos H." last="Van Peteghem">Carlos H. Van Peteghem</name></region><name sortKey="Van Bocxlaer, Jan F" sort="Van Bocxlaer, Jan F" uniqKey="Van Bocxlaer J" first="Jan F." last="Van Bocxlaer">Jan F. Van Bocxlaer</name><name sortKey="Van Bocxlaer, Jan F" sort="Van Bocxlaer, Jan F" uniqKey="Van Bocxlaer J" first="Jan F." last="Van Bocxlaer">Jan F. Van Bocxlaer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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