Characterization of p 24 Gene of Spodoptera litura Multicapsid Nucleopolyhedrovirus
Identifieur interne : 003014 ( Main/Exploration ); précédent : 003013; suivant : 003015Characterization of p 24 Gene of Spodoptera litura Multicapsid Nucleopolyhedrovirus
Auteurs : Zhaofei Li [République populaire de Chine] ; Cuifeng Li [République populaire de Chine] ; Lijing Pan [République populaire de Chine] ; Mei Yu [République populaire de Chine] ; Kai Yang [République populaire de Chine] ; Yi Pang [République populaire de Chine]Source :
- Virus Genes [ 0920-8569 ] ; 2005-05-01.
English descriptors
Abstract
Abstract: Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) p24 gene is 753 bp long, potentially encoding 244 amino acids with a predicted molecular weight of 27.3 kDa. Homology analysis indicated that SpltMNPV P24 has 20–36% amino acid identity with that of other known baculoviruses. RT-PCR results showed that the p24 gene is transcribed actively at the late stage of infection and the mRNA start site was mapped within a consensus baculovirus late promoter sequence (ATAAG). Western blot analysis of extracts from SpltMNPV-infected S. litura cells detected a specific 28 kDa protein, and this protein was not N-glycosylated. Structural localization revealed that SpltMNPV P24 was associated with the nucleocapsid of occlusion-derived virus (ODV) as a complex form of 83 kDa.
Url:
DOI: 10.1007/s11262-004-6778-8
Affiliations:
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type="abstract" xml:lang="en">Abstract: Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) p24 gene is 753 bp long, potentially encoding 244 amino acids with a predicted molecular weight of 27.3 kDa. Homology analysis indicated that SpltMNPV P24 has 20–36% amino acid identity with that of other known baculoviruses. RT-PCR results showed that the p24 gene is transcribed actively at the late stage of infection and the mRNA start site was mapped within a consensus baculovirus late promoter sequence (ATAAG). Western blot analysis of extracts from SpltMNPV-infected S. litura cells detected a specific 28 kDa protein, and this protein was not N-glycosylated. Structural localization revealed that SpltMNPV P24 was associated with the nucleocapsid of occlusion-derived virus (ODV) as a complex form of 83 kDa.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Li, Zhaofei" sort="Li, Zhaofei" uniqKey="Li Z" first="Zhaofei" last="Li">Zhaofei Li</name></region><name sortKey="Li, Cuifeng" sort="Li, Cuifeng" uniqKey="Li C" first="Cuifeng" last="Li">Cuifeng Li</name><name sortKey="Pan, Lijing" sort="Pan, Lijing" uniqKey="Pan L" first="Lijing" last="Pan">Lijing Pan</name><name sortKey="Pang, Yi" sort="Pang, Yi" uniqKey="Pang Y" first="Yi" last="Pang">Yi Pang</name><name sortKey="Pang, Yi" sort="Pang, Yi" uniqKey="Pang Y" first="Yi" last="Pang">Yi Pang</name><name sortKey="Yang, Kai" sort="Yang, Kai" uniqKey="Yang K" first="Kai" last="Yang">Kai Yang</name><name sortKey="Yu, Mei" sort="Yu, Mei" uniqKey="Yu M" first="Mei" last="Yu">Mei Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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