Polycationic Block Copolymers of Poly(ethylene oxide) and Poly(propylene oxide) for Cell Transfection
Identifieur interne : 002F25 ( Main/Exploration ); précédent : 002F24; suivant : 002F26Polycationic Block Copolymers of Poly(ethylene oxide) and Poly(propylene oxide) for Cell Transfection
Auteurs : Lev Bromberg [Espagne] ; Smeet Deshmukh [Espagne] ; Marina Temchenko [Espagne] ; Ludmila Iourtchenko [Espagne] ; Valery Alakhov [Espagne] ; Carmen Alvarez-Lorenzo [Espagne] ; Rafael Barreiro-Iglesias [Espagne] ; Angel Concheiro [Espagne] ; T. Alan Hatton [Espagne]Source :
- Bioconjugate Chemistry [ 1043-1802 ] ; 2005.
Abstract
A facile, one-step synthesis of cationic block copolymers of poly(2-N-(dimethylaminoethyl) methacrylate) (pDMAEMA) and copolymers of poly(propylene oxide) (PPO) and poly(ethylene oxide) (PEO) has been developed. The PEO−PPO−PEO−pDMAEMA (L92−pDMAEMA) and PEO−pDMAEMA copolymers were obtained via free radical polymerization of DMAEMA initiated by polyether radicals generated by cerium(IV). Over 95% of the copolymer fraction was of molecular mass ranging from 6.9 to 7.1 kDa in size, indicating the prevalence of the polyether-monoradical initiation mechanism. The L92−pDMAEMA copolymers possess parent surfactant-like surface activity. In contrast, the PEO−pDMAEMA copolymers lack significant surface activity. Both copolymers can complex with DNA. Hydrodynamic radii of the complexes of the L92−pDMAEMA and PEO−pDMAEMA with plasmid DNA ranged in size from 60 to 400 nm, depending on the copolymer/DNA ratio. Addition of Pluronic P123 to the L92−pDMAEMA complexes with DNA masked charges and decreased the tendency of the complex to aggregate, even at stoichiometric polycation/DNA ratios. The transfection efficiency of the L92−pDMAEMA copolymer was by far greater than that of the PEO−pDMAEMA copolymer. An extra added Pluronic P123 further increased the transfecton efficacy of L92−pDMAEMA, but did not affect that of PEO−pDMAEMA.
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DOI: 10.1021/bc049749f
Affiliations:
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Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139,Supratek Pharma, Inc., 531 Boulevard des Prairies, Building 18, Laval, Québec, Canada H7V 1B7, andDepartamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago deCompostela, 15872 Santiago de Compostela</wicri:regionArea><placeName><region nuts="2" type="communauté">Galice</region></placeName></affiliation><affiliation></affiliation></author><author><name sortKey="Temchenko, Marina" sort="Temchenko, Marina" uniqKey="Temchenko M" first="Marina" last="Temchenko">Marina Temchenko</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139,Supratek Pharma, Inc., 531 Boulevard des Prairies, Building 18, Laval, Québec, Canada H7V 1B7, andDepartamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago 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first="Valery" last="Alakhov">Valery Alakhov</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139,Supratek Pharma, Inc., 531 Boulevard des Prairies, Building 18, Laval, Québec, Canada H7V 1B7, andDepartamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago deCompostela, 15872 Santiago de Compostela</wicri:regionArea><placeName><region nuts="2" type="communauté">Galice</region></placeName></affiliation><affiliation></affiliation></author><author><name sortKey="Alvarez Lorenzo, Carmen" sort="Alvarez Lorenzo, Carmen" uniqKey="Alvarez Lorenzo C" first="Carmen" last="Alvarez-Lorenzo">Carmen Alvarez-Lorenzo</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139,Supratek 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Alan" last="Hatton">T. Alan Hatton</name><affiliation wicri:level="2"><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139,Supratek Pharma, Inc., 531 Boulevard des Prairies, Building 18, Laval, Québec, Canada H7V 1B7, andDepartamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago deCompostela, 15872 Santiago de Compostela</wicri:regionArea><placeName><region nuts="2" type="communauté">Galice</region></placeName></affiliation><affiliation></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Bioconjugate Chemistry</title><title level="j" type="abbrev">Bioconjugate Chem.</title><idno type="ISSN">1043-1802</idno><idno type="eISSN">1520-4812</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published">2005</date><date type="published">2005</date><biblScope unit="vol">16</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="626">626</biblScope><biblScope unit="page" to="633">633</biblScope></imprint><idno type="ISSN">1043-1802</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1043-1802</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">A facile, one-step synthesis of cationic block copolymers of poly(2-N-(dimethylaminoethyl) methacrylate) (pDMAEMA) and copolymers of poly(propylene oxide) (PPO) and poly(ethylene oxide) (PEO) has been developed. The PEO−PPO−PEO−pDMAEMA (L92−pDMAEMA) and PEO−pDMAEMA copolymers were obtained via free radical polymerization of DMAEMA initiated by polyether radicals generated by cerium(IV). Over 95% of the copolymer fraction was of molecular mass ranging from 6.9 to 7.1 kDa in size, indicating the prevalence of the polyether-monoradical initiation mechanism. The L92−pDMAEMA copolymers possess parent surfactant-like surface activity. In contrast, the PEO−pDMAEMA copolymers lack significant surface activity. Both copolymers can complex with DNA. Hydrodynamic radii of the complexes of the L92−pDMAEMA and PEO−pDMAEMA with plasmid DNA ranged in size from 60 to 400 nm, depending on the copolymer/DNA ratio. Addition of Pluronic P123 to the L92−pDMAEMA complexes with DNA masked charges and decreased the tendency of the complex to aggregate, even at stoichiometric polycation/DNA ratios. The transfection efficiency of the L92−pDMAEMA copolymer was by far greater than that of the PEO−pDMAEMA copolymer. An extra added Pluronic P123 further increased the transfecton efficacy of L92−pDMAEMA, but did not affect that of PEO−pDMAEMA.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Galice</li></region></list><tree><country name="Espagne"><region name="Galice"><name sortKey="Bromberg, Lev" sort="Bromberg, Lev" uniqKey="Bromberg L" first="Lev" last="Bromberg">Lev Bromberg</name></region><name sortKey="Alakhov, Valery" sort="Alakhov, Valery" uniqKey="Alakhov V" first="Valery" last="Alakhov">Valery Alakhov</name><name sortKey="Alvarez Lorenzo, Carmen" sort="Alvarez Lorenzo, Carmen" uniqKey="Alvarez Lorenzo C" first="Carmen" last="Alvarez-Lorenzo">Carmen Alvarez-Lorenzo</name><name sortKey="Barreiro Iglesias, Rafael" sort="Barreiro Iglesias, Rafael" uniqKey="Barreiro Iglesias R" first="Rafael" last="Barreiro-Iglesias">Rafael Barreiro-Iglesias</name><name sortKey="Concheiro, Angel" sort="Concheiro, Angel" uniqKey="Concheiro A" first="Angel" last="Concheiro">Angel Concheiro</name><name sortKey="Deshmukh, Smeet" sort="Deshmukh, Smeet" uniqKey="Deshmukh S" first="Smeet" last="Deshmukh">Smeet Deshmukh</name><name sortKey="Hatton, T Alan" sort="Hatton, T Alan" uniqKey="Hatton T" first="T. Alan" last="Hatton">T. Alan Hatton</name><name sortKey="Iourtchenko, Ludmila" sort="Iourtchenko, Ludmila" uniqKey="Iourtchenko L" first="Ludmila" last="Iourtchenko">Ludmila Iourtchenko</name><name sortKey="Temchenko, Marina" sort="Temchenko, Marina" uniqKey="Temchenko M" first="Marina" last="Temchenko">Marina Temchenko</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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