MersV1, Main, Exploration, bibRecord, 002F09

Fluorous affinity purification of oligonucleotides.

Identifieur interne : 002F09 ( Main/Exploration ); précédent : 002F08; suivant : 002F10

Fluorous affinity purification of oligonucleotides.

Auteurs : William H. Pearson [États-Unis] ; David A. Berry ; Patrick Stoy ; Kee-Yong Jung ; Anthony D. Sercel

Source :

The Journal of organic chemistry [ 0022-3263 ] ; 2005.

RBID : pubmed:16122230

Descripteurs français

KwdFr :
- Chromatographie en phase liquide à haute performance, Données de séquences moléculaires, Fluor (), Marqueurs d'affinité (), Oligonucléotides (isolement et purification), Séquence nucléotidique.
MESH :
- isolement et purification : Oligonucléotides.
- Chromatographie en phase liquide à haute performance, Données de séquences moléculaires, Fluor, Marqueurs d'affinité, Séquence nucléotidique.

English descriptors

KwdEn :
- Affinity Labels (chemistry), Base Sequence, Chromatography, High Pressure Liquid, Fluorine (chemistry), Molecular Sequence Data, Oligonucleotides (isolation & purification).
MESH :
- chemical , chemistry : Affinity Labels, Fluorine.
- chemical , isolation & purification : Oligonucleotides.
- Base Sequence, Chromatography, High Pressure Liquid, Molecular Sequence Data.

Abstract

[structure: see text] Nucleoside phosphoramidites bearing a fluorous dimethoxytrityl (FDMT) group were used to synthesize fluorous-tagged oligonucleotides, which were subjected to solid-phase extraction using a pH-stable fluorinated adsorbent. On-column detritylation afforded the purified oligonucleotides. The fluorous affinity purification method offers one-pass loading without ammonia removal, high selectivity for the removal of failure sequences, high recoveries (typically 70-100%), and the ability to purify long oligonucleotides (e.g., 50-100-mers).

DOI: 10.1021/jo050795y
PubMed: 16122230

Affiliations:

États-Unis

Le document en format XML

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<author><name sortKey="Berry, David A" sort="Berry, David A" uniqKey="Berry D" first="David A" last="Berry">David A. Berry</name>
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<author><name sortKey="Stoy, Patrick" sort="Stoy, Patrick" uniqKey="Stoy P" first="Patrick" last="Stoy">Patrick Stoy</name>
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<author><name sortKey="Jung, Kee Yong" sort="Jung, Kee Yong" uniqKey="Jung K" first="Kee-Yong" last="Jung">Kee-Yong Jung</name>
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<author><name sortKey="Sercel, Anthony D" sort="Sercel, Anthony D" uniqKey="Sercel A" first="Anthony D" last="Sercel">Anthony D. Sercel</name>
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<author><name sortKey="Jung, Kee Yong" sort="Jung, Kee Yong" uniqKey="Jung K" first="Kee-Yong" last="Jung">Kee-Yong Jung</name>
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<series><title level="j">The Journal of organic chemistry</title>
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<term>Molecular Sequence Data</term>
<term>Oligonucleotides (isolation & purification)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term>
<term>Données de séquences moléculaires</term>
<term>Fluor ()</term>
<term>Marqueurs d'affinité ()</term>
<term>Oligonucléotides (isolement et purification)</term>
<term>Séquence nucléotidique</term>
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<term>Données de séquences moléculaires</term>
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<front><div type="abstract" xml:lang="en">[structure: see text] Nucleoside phosphoramidites bearing a fluorous dimethoxytrityl (FDMT) group were used to synthesize fluorous-tagged oligonucleotides, which were subjected to solid-phase extraction using a pH-stable fluorinated adsorbent. On-column detritylation afforded the purified oligonucleotides. The fluorous affinity purification method offers one-pass loading without ammonia removal, high selectivity for the removal of failure sequences, high recoveries (typically 70-100%), and the ability to purify long oligonucleotides (e.g., 50-100-mers).</div>
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<name sortKey="Sercel, Anthony D" sort="Sercel, Anthony D" uniqKey="Sercel A" first="Anthony D" last="Sercel">Anthony D. Sercel</name>
<name sortKey="Stoy, Patrick" sort="Stoy, Patrick" uniqKey="Stoy P" first="Patrick" last="Stoy">Patrick Stoy</name>
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<country name="États-Unis"><noRegion><name sortKey="Pearson, William H" sort="Pearson, William H" uniqKey="Pearson W" first="William H" last="Pearson">William H. Pearson</name>
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Serveur d'exploration MERS

Fluorous affinity purification of oligonucleotides.

Fluorous affinity purification of oligonucleotides.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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