Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9).
Identifieur interne : 002E82 ( Main/Exploration ); précédent : 002E81; suivant : 002E83Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9).
Auteurs : Justina Clarinda Wolters [Allemagne] ; Rupert Abele ; Robert TampéSource :
- The Journal of biological chemistry [ 0021-9258 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : ATP-Binding Cassette Transporters.
- chemical , metabolism : ATP-Binding Cassette Transporters, Adenosine Triphosphate, Peptides.
- Base Sequence, DNA Primers, Dimerization, Humans, Kinetics, Protein Transport.
Abstract
The transporter associated with antigen processing (TAP)-like (TAPL, ABCB9) belongs to the ATP-binding cassette transporter family, which translocates a vast variety of solutes across membranes. The function of this half-size transporter has not yet been determined. Here, we show that TAPL forms a homodimeric complex, which translocates peptides across the membrane. Peptide transport strictly requires ATP hydrolysis. The transport follows Michaelis-Menten kinetics with low affinity and high capacity. Different nucleotides bind and energize the transport with a slight predilection for purine bases. The peptide specificity is very broad, ranging from 6-mer up to at least 59-mer peptides with a preference for 23-mers. Peptides are recognized via their backbone, including the free N and C termini as well as side chain interactions. Although related to TAP, TAPL is unique as far as its interaction partners, transport properties, and substrate specificities are concerned, thus excluding that TAPL is part of the peptide-loading complex in the classic route of antigen processing via major histocompatibility complex class I molecules.
DOI: 10.1074/jbc.M503231200
PubMed: 15863492
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002338
- to stream PubMed, to step Curation: 002338
- to stream PubMed, to step Checkpoint: 002180
- to stream Ncbi, to step Merge: 000340
- to stream Ncbi, to step Curation: 000340
- to stream Ncbi, to step Checkpoint: 000340
- to stream Main, to step Merge: 002F12
- to stream Main, to step Curation: 002E82
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9).</title>
<author><name sortKey="Wolters, Justina Clarinda" sort="Wolters, Justina Clarinda" uniqKey="Wolters J" first="Justina Clarinda" last="Wolters">Justina Clarinda Wolters</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Biochemistry, Biocenter, Johann Wolfgang Goethe-University Frankfurt, Marie-Curie-Str. 9, D-60439 Frankfurt/M., Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry, Biocenter, Johann Wolfgang Goethe-University Frankfurt, Marie-Curie-Str. 9, D-60439 Frankfurt/M.</wicri:regionArea>
<placeName><region type="land" nuts="1">Hesse (Land)</region>
<region type="district" nuts="2">District de Darmstadt</region>
<settlement type="city">Francfort-sur-le-Main</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Abele, Rupert" sort="Abele, Rupert" uniqKey="Abele R" first="Rupert" last="Abele">Rupert Abele</name>
</author>
<author><name sortKey="Tampe, Robert" sort="Tampe, Robert" uniqKey="Tampe R" first="Robert" last="Tampé">Robert Tampé</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:15863492</idno>
<idno type="pmid">15863492</idno>
<idno type="doi">10.1074/jbc.M503231200</idno>
<idno type="wicri:Area/PubMed/Corpus">002338</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002338</idno>
<idno type="wicri:Area/PubMed/Curation">002338</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002338</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002180</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002180</idno>
<idno type="wicri:Area/Ncbi/Merge">000340</idno>
<idno type="wicri:Area/Ncbi/Curation">000340</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000340</idno>
<idno type="wicri:doubleKey">0021-9258:2005:Wolters J:selective:and:atp</idno>
<idno type="wicri:Area/Main/Merge">002F12</idno>
<idno type="wicri:Area/Main/Curation">002E82</idno>
<idno type="wicri:Area/Main/Exploration">002E82</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9).</title>
<author><name sortKey="Wolters, Justina Clarinda" sort="Wolters, Justina Clarinda" uniqKey="Wolters J" first="Justina Clarinda" last="Wolters">Justina Clarinda Wolters</name>
<affiliation wicri:level="3"><nlm:affiliation>Institute of Biochemistry, Biocenter, Johann Wolfgang Goethe-University Frankfurt, Marie-Curie-Str. 9, D-60439 Frankfurt/M., Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry, Biocenter, Johann Wolfgang Goethe-University Frankfurt, Marie-Curie-Str. 9, D-60439 Frankfurt/M.</wicri:regionArea>
<placeName><region type="land" nuts="1">Hesse (Land)</region>
<region type="district" nuts="2">District de Darmstadt</region>
<settlement type="city">Francfort-sur-le-Main</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Abele, Rupert" sort="Abele, Rupert" uniqKey="Abele R" first="Rupert" last="Abele">Rupert Abele</name>
</author>
<author><name sortKey="Tampe, Robert" sort="Tampe, Robert" uniqKey="Tampe R" first="Robert" last="Tampé">Robert Tampé</name>
</author>
</analytic>
<series><title level="j">The Journal of biological chemistry</title>
<idno type="ISSN">0021-9258</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ATP-Binding Cassette Transporters (chemistry)</term>
<term>ATP-Binding Cassette Transporters (metabolism)</term>
<term>Adenosine Triphosphate (metabolism)</term>
<term>Base Sequence</term>
<term>DNA Primers</term>
<term>Dimerization</term>
<term>Humans</term>
<term>Kinetics</term>
<term>Peptides (metabolism)</term>
<term>Protein Transport</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adénosine triphosphate (métabolisme)</term>
<term>Amorces ADN</term>
<term>Cinétique</term>
<term>Dimérisation</term>
<term>Humains</term>
<term>Peptides (métabolisme)</term>
<term>Séquence nucléotidique</term>
<term>Transport de protéines</term>
<term>Transporteurs ABC ()</term>
<term>Transporteurs ABC (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>ATP-Binding Cassette Transporters</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>ATP-Binding Cassette Transporters</term>
<term>Adenosine Triphosphate</term>
<term>Peptides</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adénosine triphosphate</term>
<term>Peptides</term>
<term>Transporteurs ABC</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>DNA Primers</term>
<term>Dimerization</term>
<term>Humans</term>
<term>Kinetics</term>
<term>Protein Transport</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term>
<term>Cinétique</term>
<term>Dimérisation</term>
<term>Humains</term>
<term>Séquence nucléotidique</term>
<term>Transport de protéines</term>
<term>Transporteurs ABC</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The transporter associated with antigen processing (TAP)-like (TAPL, ABCB9) belongs to the ATP-binding cassette transporter family, which translocates a vast variety of solutes across membranes. The function of this half-size transporter has not yet been determined. Here, we show that TAPL forms a homodimeric complex, which translocates peptides across the membrane. Peptide transport strictly requires ATP hydrolysis. The transport follows Michaelis-Menten kinetics with low affinity and high capacity. Different nucleotides bind and energize the transport with a slight predilection for purine bases. The peptide specificity is very broad, ranging from 6-mer up to at least 59-mer peptides with a preference for 23-mers. Peptides are recognized via their backbone, including the free N and C termini as well as side chain interactions. Although related to TAP, TAPL is unique as far as its interaction partners, transport properties, and substrate specificities are concerned, thus excluding that TAPL is part of the peptide-loading complex in the classic route of antigen processing via major histocompatibility complex class I molecules.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
</country>
<region><li>District de Darmstadt</li>
<li>Hesse (Land)</li>
</region>
<settlement><li>Francfort-sur-le-Main</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Abele, Rupert" sort="Abele, Rupert" uniqKey="Abele R" first="Rupert" last="Abele">Rupert Abele</name>
<name sortKey="Tampe, Robert" sort="Tampe, Robert" uniqKey="Tampe R" first="Robert" last="Tampé">Robert Tampé</name>
</noCountry>
<country name="Allemagne"><region name="Hesse (Land)"><name sortKey="Wolters, Justina Clarinda" sort="Wolters, Justina Clarinda" uniqKey="Wolters J" first="Justina Clarinda" last="Wolters">Justina Clarinda Wolters</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002E82 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002E82 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:15863492 |texte= Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9). }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15863492" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
This area was generated with Dilib version V0.6.33. |