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Solid sampling technique for direct detection of condensed tannins in bark by matrix-assisted laser desorption/ionization mass spectrometry.

Identifieur interne : 002E76 ( Main/Exploration ); précédent : 002E75; suivant : 002E77

Solid sampling technique for direct detection of condensed tannins in bark by matrix-assisted laser desorption/ionization mass spectrometry.

Auteurs : Yasuyuki Ishida [Japon] ; Kuniyuki Kitagawa ; Kuniyoshi Goto ; Hajime Ohtani

Source :

RBID : pubmed:15702488

Descripteurs français

English descriptors

Abstract

A novel method for the direct analysis of condensed tannin components in bark was developed on the basis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with a simple solid sampling technique. The MALDI mass spectra obtained from the wood (bark) powder sample clearly showed a series of peaks corresponding to the sodium ion adducts of condensed tannin oligomers up to around m/z 3000. The results indicate that the condensed tannins in the bark sample used in this work mostly consist of combinations of flavan-3-ol units such as profisetinidin (PF), prorobinetinidin (PR) and prodelphinidin (PD), at least up to 10-mers (m/z approximately 3000).

DOI: 10.1002/rcm.1845
PubMed: 15702488


Affiliations:


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Le document en format XML

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<term>Acacia (metabolism)</term>
<term>Plant Bark (metabolism)</term>
<term>Plant Extracts (analysis)</term>
<term>Plant Extracts (chemistry)</term>
<term>Specimen Handling (methods)</term>
<term>Spectrometry, Mass, Electrospray Ionization (methods)</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)</term>
<term>Tannins (analysis)</term>
<term>Tannins (chemistry)</term>
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<term>Acacia (métabolisme)</term>
<term>Bois</term>
<term>Extraits de plantes ()</term>
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<term>Prélèvement biologique ()</term>
<term>Spectrométrie de masse ESI ()</term>
<term>Spectrométrie de masse MALDI ()</term>
<term>Tanins ()</term>
<term>Tanins (analyse)</term>
<term>Écorce (métabolisme)</term>
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<div type="abstract" xml:lang="en">A novel method for the direct analysis of condensed tannin components in bark was developed on the basis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with a simple solid sampling technique. The MALDI mass spectra obtained from the wood (bark) powder sample clearly showed a series of peaks corresponding to the sodium ion adducts of condensed tannin oligomers up to around m/z 3000. The results indicate that the condensed tannins in the bark sample used in this work mostly consist of combinations of flavan-3-ol units such as profisetinidin (PF), prorobinetinidin (PR) and prodelphinidin (PD), at least up to 10-mers (m/z approximately 3000).</div>
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