Cytotoxicity of albebetin oligomers depends on cross-beta-sheet formation.
Identifieur interne : 002D42 ( Main/Exploration ); précédent : 002D41; suivant : 002D43Cytotoxicity of albebetin oligomers depends on cross-beta-sheet formation.
Auteurs : Vladimir Zamotin [Suède] ; Anna Gharibyan ; Natalia V. Gibanova ; Marika A. Lavrikova ; Dmitry A. Dolgikh ; Michail P. Kirpichnikov ; Irina A. Kostanyan ; Ludmilla A. Morozova-RocheSource :
- FEBS letters [ 0014-5793 ] ; 2006.
Descripteurs français
- KwdFr :
- Amyloïde (métabolisme), Animaux, Biopolymères (), Biopolymères (toxicité), Cellules cultivées, Cervelet (), Cervelet (cytologie), Cervelet (métabolisme), Dichroïsme circulaire, Microscopie à force atomique, Mâle, Neurones (), Neurones (métabolisme), Protéines (), Protéines (toxicité), Protéines recombinantes (), Protéines recombinantes (toxicité), Rat Wistar, Rats, Spectrophotométrie UV.
- MESH :
- cytologie : Cervelet.
- métabolisme : Amyloïde, Cervelet, Neurones.
- toxicité : Biopolymères, Protéines, Protéines recombinantes.
- Animaux, Biopolymères, Cellules cultivées, Cervelet, Dichroïsme circulaire, Microscopie à force atomique, Mâle, Neurones, Protéines, Protéines recombinantes, Rat Wistar, Rats, Spectrophotométrie UV.
English descriptors
- KwdEn :
- Amyloid (metabolism), Animals, Biopolymers (chemistry), Biopolymers (toxicity), Cells, Cultured, Cerebellum (cytology), Cerebellum (drug effects), Cerebellum (metabolism), Circular Dichroism, Male, Microscopy, Atomic Force, Neurons (drug effects), Neurons (metabolism), Proteins (chemistry), Proteins (toxicity), Rats, Rats, Wistar, Recombinant Proteins (chemistry), Recombinant Proteins (toxicity), Spectrophotometry, Ultraviolet.
- MESH :
- chemical , chemistry : Biopolymers, Proteins, Recombinant Proteins.
- chemical , metabolism : Amyloid.
- chemical , toxicity : Biopolymers, Proteins, Recombinant Proteins.
- cytology : Cerebellum.
- drug effects : Cerebellum, Neurons.
- metabolism : Cerebellum, Neurons.
- Animals, Cells, Cultured, Circular Dichroism, Male, Microscopy, Atomic Force, Rats, Rats, Wistar, Spectrophotometry, Ultraviolet.
Abstract
Prefibrillar cytotoxicity was suggested as a common amyloid characteristic. We showed two types of albebetin prefibrillar oligomers are formed during incubation at pH 7.3. Initial round-shaped oligomers consist of 10-15 molecules determined by atomic force microscopy, do not bind thioflavin-T and do not affect viability of granular neurons and SH-SY5Y cells. They are converted into ca. 30-40-mers possessing cross-beta-sheet and reducing viability of neuronal cells. Neither monomers nor fibrils possess cytotoxicity. We suggest that oligomeric size is important for stabilising cross-beta-sheet core critical for cytotoxicity. As albebetin was used as a carrier-protein for drug delivery, examination of amyloidogenicity is required prior polypeptide biomedical applications.
DOI: 10.1016/j.febslet.2006.03.074
PubMed: 16638570
Affiliations:
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Le document en format XML
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Gibanova</name></author><author><name sortKey="Lavrikova, Marika A" sort="Lavrikova, Marika A" uniqKey="Lavrikova M" first="Marika A" last="Lavrikova">Marika A. Lavrikova</name></author><author><name sortKey="Dolgikh, Dmitry A" sort="Dolgikh, Dmitry A" uniqKey="Dolgikh D" first="Dmitry A" last="Dolgikh">Dmitry A. Dolgikh</name></author><author><name sortKey="Kirpichnikov, Michail P" sort="Kirpichnikov, Michail P" uniqKey="Kirpichnikov M" first="Michail P" last="Kirpichnikov">Michail P. Kirpichnikov</name></author><author><name sortKey="Kostanyan, Irina A" sort="Kostanyan, Irina A" uniqKey="Kostanyan I" first="Irina A" last="Kostanyan">Irina A. Kostanyan</name></author><author><name sortKey="Morozova Roche, Ludmilla A" sort="Morozova Roche, Ludmilla A" uniqKey="Morozova Roche L" first="Ludmilla A" last="Morozova-Roche">Ludmilla A. Morozova-Roche</name></author></analytic><series><title level="j">FEBS letters</title><idno type="ISSN">0014-5793</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amyloid (metabolism)</term><term>Animals</term><term>Biopolymers (chemistry)</term><term>Biopolymers (toxicity)</term><term>Cells, Cultured</term><term>Cerebellum (cytology)</term><term>Cerebellum (drug effects)</term><term>Cerebellum (metabolism)</term><term>Circular Dichroism</term><term>Male</term><term>Microscopy, Atomic Force</term><term>Neurons (drug effects)</term><term>Neurons (metabolism)</term><term>Proteins (chemistry)</term><term>Proteins (toxicity)</term><term>Rats</term><term>Rats, Wistar</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (toxicity)</term><term>Spectrophotometry, Ultraviolet</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amyloïde (métabolisme)</term><term>Animaux</term><term>Biopolymères ()</term><term>Biopolymères (toxicité)</term><term>Cellules cultivées</term><term>Cervelet ()</term><term>Cervelet (cytologie)</term><term>Cervelet (métabolisme)</term><term>Dichroïsme circulaire</term><term>Microscopie à force atomique</term><term>Mâle</term><term>Neurones ()</term><term>Neurones (métabolisme)</term><term>Protéines ()</term><term>Protéines (toxicité)</term><term>Protéines recombinantes ()</term><term>Protéines recombinantes (toxicité)</term><term>Rat Wistar</term><term>Rats</term><term>Spectrophotométrie UV</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Biopolymers</term><term>Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amyloid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Biopolymers</term><term>Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cervelet</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Cerebellum</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cerebellum</term><term>Neurons</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cerebellum</term><term>Neurons</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Amyloïde</term><term>Cervelet</term><term>Neurones</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Biopolymères</term><term>Protéines</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Circular Dichroism</term><term>Male</term><term>Microscopy, Atomic Force</term><term>Rats</term><term>Rats, Wistar</term><term>Spectrophotometry, Ultraviolet</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Biopolymères</term><term>Cellules cultivées</term><term>Cervelet</term><term>Dichroïsme circulaire</term><term>Microscopie à force atomique</term><term>Mâle</term><term>Neurones</term><term>Protéines</term><term>Protéines recombinantes</term><term>Rat Wistar</term><term>Rats</term><term>Spectrophotométrie UV</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Prefibrillar cytotoxicity was suggested as a common amyloid characteristic. We showed two types of albebetin prefibrillar oligomers are formed during incubation at pH 7.3. Initial round-shaped oligomers consist of 10-15 molecules determined by atomic force microscopy, do not bind thioflavin-T and do not affect viability of granular neurons and SH-SY5Y cells. They are converted into ca. 30-40-mers possessing cross-beta-sheet and reducing viability of neuronal cells. Neither monomers nor fibrils possess cytotoxicity. We suggest that oligomeric size is important for stabilising cross-beta-sheet core critical for cytotoxicity. As albebetin was used as a carrier-protein for drug delivery, examination of amyloidogenicity is required prior polypeptide biomedical applications.</div></front></TEI><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Dolgikh, Dmitry A" sort="Dolgikh, Dmitry A" uniqKey="Dolgikh D" first="Dmitry A" last="Dolgikh">Dmitry A. Dolgikh</name><name sortKey="Gharibyan, Anna" sort="Gharibyan, Anna" uniqKey="Gharibyan A" first="Anna" last="Gharibyan">Anna Gharibyan</name><name sortKey="Gibanova, Natalia V" sort="Gibanova, Natalia V" uniqKey="Gibanova N" first="Natalia V" last="Gibanova">Natalia V. Gibanova</name><name sortKey="Kirpichnikov, Michail P" sort="Kirpichnikov, Michail P" uniqKey="Kirpichnikov M" first="Michail P" last="Kirpichnikov">Michail P. Kirpichnikov</name><name sortKey="Kostanyan, Irina A" sort="Kostanyan, Irina A" uniqKey="Kostanyan I" first="Irina A" last="Kostanyan">Irina A. Kostanyan</name><name sortKey="Lavrikova, Marika A" sort="Lavrikova, Marika A" uniqKey="Lavrikova M" first="Marika A" last="Lavrikova">Marika A. Lavrikova</name><name sortKey="Morozova Roche, Ludmilla A" sort="Morozova Roche, Ludmilla A" uniqKey="Morozova Roche L" first="Ludmilla A" last="Morozova-Roche">Ludmilla A. Morozova-Roche</name></noCountry><country name="Suède"><noRegion><name sortKey="Zamotin, Vladimir" sort="Zamotin, Vladimir" uniqKey="Zamotin V" first="Vladimir" last="Zamotin">Vladimir Zamotin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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