Identification of naturally processed peptides bound to the class I MHC molecule H-2Kd of normal and TAP-deficient cells.
Identifieur interne : 002D19 ( Main/Exploration ); précédent : 002D18; suivant : 002D20Identification of naturally processed peptides bound to the class I MHC molecule H-2Kd of normal and TAP-deficient cells.
Auteurs : Anish Suri [États-Unis] ; James J. Walters ; Matteo G. Levisetti ; Michael L. Gross ; Emil R. UnanueSource :
- European journal of immunology [ 0014-2980 ] ; 2006.
Descripteurs français
- KwdFr :
- Acides aminés (immunologie), Animaux, Antigènes H-2 (immunologie), Antigènes Ly (immunologie), Liaison aux protéines (immunologie), Lignée cellulaire, Maturation post-traductionnelle des protéines (immunologie), Peptides (immunologie), Protéines membranaires (déficit), Protéines membranaires (immunologie), Présentation d'antigène (génétique), Présentation d'antigène (immunologie), Souris, Souris de lignée NOD.
- MESH :
- déficit : Protéines membranaires.
- génétique : Présentation d'antigène.
- immunologie : Acides aminés, Antigènes H-2, Antigènes Ly, Liaison aux protéines, Maturation post-traductionnelle des protéines, Peptides, Protéines membranaires, Présentation d'antigène.
- Animaux, Lignée cellulaire, Souris, Souris de lignée NOD.
English descriptors
- KwdEn :
- Amino Acids (immunology), Animals, Antigen Presentation (genetics), Antigen Presentation (immunology), Antigens, Ly (immunology), Cell Line, H-2 Antigens (immunology), Membrane Proteins (deficiency), Membrane Proteins (immunology), Mice, Mice, Inbred NOD, Peptides (immunology), Protein Binding (immunology), Protein Processing, Post-Translational (immunology).
- MESH :
- chemical , deficiency : Membrane Proteins.
- chemical , immunology : Amino Acids, Antigens, Ly, H-2 Antigens, Membrane Proteins, Peptides.
- genetics : Antigen Presentation.
- immunology : Antigen Presentation, Protein Binding, Protein Processing, Post-Translational.
- Animals, Cell Line, Mice, Mice, Inbred NOD.
Abstract
This report details the biochemical features of natural peptides selected by the H-2Kd class I MHC molecule. In normal cell lines, the length of the naturally processed peptides ranged from 8 to 18 amino acids, although the majority were 9-mers (16% were longer than nine residues). The binding motif for the 9-mer peptides was dominated by the presence of a tyrosine at P2 and an isoleucine/leucine at the P9 position. The P2 residue contributed most towards binding; and the short peptides bound better and formed longer-lived cell surface complexes than the long peptides, which bound poorly and dissociated rapidly. The longer peptides did not exhibit this strictly defined motif. Trimming the long peptides to their shorter forms did not enhance binding and conversely, extending the 9-mer peptides did not decrease binding. The long peptides were present on the cell-surface bound to H-2Kd (Kd) and were not intermediate products of the class I MHC processing pathway. Finally, in two different TAP-deficient cells the long peptides were the dominant species, which suggested that TAP-independent pathways selected for long peptides by class I MHC molecules.
DOI: 10.1002/eji.200526235
PubMed: 16479539
Affiliations:
- États-Unis
- Missouri (État)
- Saint-Louis (Missouri)
- École de médecine (Université Washington de Saint-Louis)
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Le document en format XML
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Unanue</name></author></analytic><series><title level="j">European journal of immunology</title><idno type="ISSN">0014-2980</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (immunology)</term><term>Animals</term><term>Antigen Presentation (genetics)</term><term>Antigen Presentation (immunology)</term><term>Antigens, Ly (immunology)</term><term>Cell Line</term><term>H-2 Antigens (immunology)</term><term>Membrane Proteins (deficiency)</term><term>Membrane Proteins (immunology)</term><term>Mice</term><term>Mice, Inbred NOD</term><term>Peptides (immunology)</term><term>Protein Binding (immunology)</term><term>Protein Processing, Post-Translational (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides aminés (immunologie)</term><term>Animaux</term><term>Antigènes H-2 (immunologie)</term><term>Antigènes Ly (immunologie)</term><term>Liaison aux protéines (immunologie)</term><term>Lignée cellulaire</term><term>Maturation post-traductionnelle des protéines (immunologie)</term><term>Peptides (immunologie)</term><term>Protéines membranaires (déficit)</term><term>Protéines membranaires (immunologie)</term><term>Présentation d'antigène (génétique)</term><term>Présentation d'antigène (immunologie)</term><term>Souris</term><term>Souris de lignée NOD</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Membrane Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Amino Acids</term><term>Antigens, Ly</term><term>H-2 Antigens</term><term>Membrane Proteins</term><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Protéines membranaires</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Antigen Presentation</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Présentation d'antigène</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Acides aminés</term><term>Antigènes H-2</term><term>Antigènes Ly</term><term>Liaison aux protéines</term><term>Maturation post-traductionnelle des protéines</term><term>Peptides</term><term>Protéines membranaires</term><term>Présentation d'antigène</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Antigen Presentation</term><term>Protein Binding</term><term>Protein Processing, Post-Translational</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Mice</term><term>Mice, Inbred NOD</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Lignée cellulaire</term><term>Souris</term><term>Souris de lignée NOD</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This report details the biochemical features of natural peptides selected by the H-2Kd class I MHC molecule. In normal cell lines, the length of the naturally processed peptides ranged from 8 to 18 amino acids, although the majority were 9-mers (16% were longer than nine residues). The binding motif for the 9-mer peptides was dominated by the presence of a tyrosine at P2 and an isoleucine/leucine at the P9 position. The P2 residue contributed most towards binding; and the short peptides bound better and formed longer-lived cell surface complexes than the long peptides, which bound poorly and dissociated rapidly. The longer peptides did not exhibit this strictly defined motif. Trimming the long peptides to their shorter forms did not enhance binding and conversely, extending the 9-mer peptides did not decrease binding. The long peptides were present on the cell-surface bound to H-2Kd (Kd) and were not intermediate products of the class I MHC processing pathway. Finally, in two different TAP-deficient cells the long peptides were the dominant species, which suggested that TAP-independent pathways selected for long peptides by class I MHC molecules.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Missouri (État)</li></region><settlement><li>Saint-Louis (Missouri)</li></settlement><orgName><li>École de médecine (Université Washington de Saint-Louis)</li></orgName></list><tree><noCountry><name sortKey="Gross, Michael L" sort="Gross, Michael L" uniqKey="Gross M" first="Michael L" last="Gross">Michael L. Gross</name><name sortKey="Levisetti, Matteo G" sort="Levisetti, Matteo G" uniqKey="Levisetti M" first="Matteo G" last="Levisetti">Matteo G. Levisetti</name><name sortKey="Unanue, Emil R" sort="Unanue, Emil R" uniqKey="Unanue E" first="Emil R" last="Unanue">Emil R. Unanue</name><name sortKey="Walters, James J" sort="Walters, James J" uniqKey="Walters J" first="James J" last="Walters">James J. Walters</name></noCountry><country name="États-Unis"><region name="Missouri (État)"><name sortKey="Suri, Anish" sort="Suri, Anish" uniqKey="Suri A" first="Anish" last="Suri">Anish Suri</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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