Sub-epitopic dissection of HCV E1315–328HRMAWDMMMNWSPT sequence by similarity analysis
Identifieur interne : 002C23 ( Main/Exploration ); précédent : 002C22; suivant : 002C24Sub-epitopic dissection of HCV E1315–328HRMAWDMMMNWSPT sequence by similarity analysis
Auteurs : L. Polimeno [Italie] ; A. Mittelman [États-Unis] ; L. Gennero [Italie] ; A. Ponzetto [Italie] ; G. Lucchese [Italie] ; A. Stufano [Italie] ; A. Kusalik [Canada] ; D. Kanduc [Italie]Source :
- Amino Acids [ 0939-4451 ] ; 2008-04-01.
English descriptors
Abstract
Summary.: Our labs are focused on identifying amino acid sequences having the ability to react specifically with the functional binding site of a complementary antibody. Our epitopic definition is based on the analysis of the similarity level of antigenic amino acid sequences to the host proteome. Here, the similarity profile to the human proteome of an HCV E1 immunodominant epitope, i.e. the HCV E1315–328HRMAWDMMMNWSPT sequence, led to i) characterizing the immunoreactive HCV E1 315–328 region as a sequence endowed with a low level of similarity to human proteins; ii) defining 2 contiguous immunodominant linear determinants respectively located at the NH2 and COOH terminus of the conserved viral antigenic sequence. This study supports the hypothesis that low sequence similarity to the host’s proteome modulates the pool of epitopic amino acid sequences in a viral antigen, and appears of potential value in defining immunogenic viral peptide sequences to be used in immunotherapeutic approaches for HCV treatment.
Url:
DOI: 10.1007/s00726-007-0539-7
Affiliations:
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Polimeno</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Emergency and Organ Transplantation, Gastroenterology Section, University of Bari, Bari</wicri:regionArea><wicri:noRegion>Bari</wicri:noRegion></affiliation></author><author><name sortKey="Mittelman, A" sort="Mittelman, A" uniqKey="Mittelman A" first="A." last="Mittelman">A. Mittelman</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Oncology/Hematology, Department of Medicine, and Department of Microbiology and Immunology, New York Medical College, Valhalla</wicri:regionArea><wicri:noRegion>Valhalla</wicri:noRegion></affiliation></author><author><name sortKey="Gennero, L" sort="Gennero, L" uniqKey="Gennero L" first="L." last="Gennero">L. Gennero</name><affiliation wicri:level="3"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Internal Medicine, University of Turin, Turin</wicri:regionArea><placeName><settlement type="city">Turin</settlement><region type="région" nuts="2">Piémont</region></placeName></affiliation></author><author><name sortKey="Ponzetto, A" sort="Ponzetto, A" uniqKey="Ponzetto A" first="A." last="Ponzetto">A. Ponzetto</name><affiliation wicri:level="3"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Internal Medicine, University of Turin, Turin</wicri:regionArea><placeName><settlement type="city">Turin</settlement><region type="région" nuts="2">Piémont</region></placeName></affiliation></author><author><name sortKey="Lucchese, G" sort="Lucchese, G" uniqKey="Lucchese G" first="G." last="Lucchese">G. Lucchese</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biochemistry and Molecular Biology “Ernesto Quagliariello”, University of Bari, Bari</wicri:regionArea><wicri:noRegion>Bari</wicri:noRegion></affiliation></author><author><name sortKey="Stufano, A" sort="Stufano, A" uniqKey="Stufano A" first="A." last="Stufano">A. Stufano</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biochemistry and Molecular Biology “Ernesto Quagliariello”, University of Bari, Bari</wicri:regionArea><wicri:noRegion>Bari</wicri:noRegion></affiliation></author><author><name sortKey="Kusalik, A" sort="Kusalik, A" uniqKey="Kusalik A" first="A." last="Kusalik">A. Kusalik</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Computer Science, University of Saskatchewan, Saskatoon</wicri:regionArea><wicri:noRegion>Saskatoon</wicri:noRegion></affiliation></author><author><name sortKey="Kanduc, D" sort="Kanduc, D" uniqKey="Kanduc D" first="D." last="Kanduc">D. Kanduc</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Biochemistry and Molecular Biology “Ernesto Quagliariello”, University of Bari, Bari</wicri:regionArea><wicri:noRegion>Bari</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Italie</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Amino Acids</title><title level="j" type="sub">The Forum for Amino Acid and Protein Research</title><title level="j" type="abbrev">Amino Acids</title><idno type="ISSN">0939-4451</idno><idno type="eISSN">1438-2199</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="2008-04-01">2008-04-01</date><biblScope unit="volume">34</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="479">479</biblScope><biblScope unit="page" to="484">484</biblScope></imprint><idno type="ISSN">0939-4451</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0939-4451</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Keywords: Epitope mapping – Similarity level – Amino acid sequence uniqueness – Computational biology – Anti-HCV peptide vaccines</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary.: Our labs are focused on identifying amino acid sequences having the ability to react specifically with the functional binding site of a complementary antibody. Our epitopic definition is based on the analysis of the similarity level of antigenic amino acid sequences to the host proteome. Here, the similarity profile to the human proteome of an HCV E1 immunodominant epitope, i.e. the HCV E1315–328HRMAWDMMMNWSPT sequence, led to i) characterizing the immunoreactive HCV E1 315–328 region as a sequence endowed with a low level of similarity to human proteins; ii) defining 2 contiguous immunodominant linear determinants respectively located at the NH2 and COOH terminus of the conserved viral antigenic sequence. This study supports the hypothesis that low sequence similarity to the host’s proteome modulates the pool of epitopic amino acid sequences in a viral antigen, and appears of potential value in defining immunogenic viral peptide sequences to be used in immunotherapeutic approaches for HCV treatment.</div></front></TEI><affiliations><list><country><li>Canada</li><li>Italie</li><li>États-Unis</li></country><region><li>Piémont</li></region><settlement><li>Turin</li></settlement></list><tree><country name="Italie"><noRegion><name sortKey="Polimeno, L" sort="Polimeno, L" uniqKey="Polimeno L" first="L." last="Polimeno">L. Polimeno</name></noRegion><name sortKey="Gennero, L" sort="Gennero, L" uniqKey="Gennero L" first="L." last="Gennero">L. Gennero</name><name sortKey="Kanduc, D" sort="Kanduc, D" uniqKey="Kanduc D" first="D." last="Kanduc">D. Kanduc</name><name sortKey="Kanduc, D" sort="Kanduc, D" uniqKey="Kanduc D" first="D." last="Kanduc">D. Kanduc</name><name sortKey="Lucchese, G" sort="Lucchese, G" uniqKey="Lucchese G" first="G." last="Lucchese">G. Lucchese</name><name sortKey="Ponzetto, A" sort="Ponzetto, A" uniqKey="Ponzetto A" first="A." last="Ponzetto">A. Ponzetto</name><name sortKey="Stufano, A" sort="Stufano, A" uniqKey="Stufano A" first="A." last="Stufano">A. Stufano</name></country><country name="États-Unis"><noRegion><name sortKey="Mittelman, A" sort="Mittelman, A" uniqKey="Mittelman A" first="A." last="Mittelman">A. Mittelman</name></noRegion></country><country name="Canada"><noRegion><name sortKey="Kusalik, A" sort="Kusalik, A" uniqKey="Kusalik A" first="A." last="Kusalik">A. Kusalik</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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