Gold‐Nanorod‐Based Sensing of Sequence Specific HIV‐1 Virus DNA by Using Hyper‐Rayleigh Scattering Spectroscopy
Identifieur interne : 002A79 ( Main/Exploration ); précédent : 002A78; suivant : 002A80Gold‐Nanorod‐Based Sensing of Sequence Specific HIV‐1 Virus DNA by Using Hyper‐Rayleigh Scattering Spectroscopy
Auteurs : Gopala Rishna Darbha ; Uma Shanker Rai ; Anant Umar Singh ; Paresh Handra Ray [États-Unis]Source :
- Chemistry – A European Journal [ 0947-6539 ] ; 2008-04-28.
English descriptors
- Teeft :
- Angew, Aqueous solution, Aqueous solutions, Aspect ratio, Aspect ratios, Assay, Base pair mismatch, Biological imaging, Biotechnological systems, Chem, Colloidal gold seeds, Conduction band electrons, Different concentration, Electromagnetic fields, Excellent sensitivity, Excitation wavelength, Experimental evidence, Experimental results show, First time, Fundamental beam, Fundamental wavelength, Gmbh, Gold nanoparticles, Gold nanorod, Gold nanorod solution, Gold nanorods, Gold nanostructures, Higher multipolar contribution, Human beings, Human immunodeficiency virus, Hybridization, Hybridization process, Intensity change, Intensity changes, Intensity increases, Kgaa, Lett, Linear nature, Long targets, Longitudinal absorption band, Longitudinal oscillation, Mismatch, Nano, Nano lett, Nanoparticles, Nanorod, Nanorods, Paper figure, Phys, Picomolar level, Pure gold nanorod solution, Raman, Resonance enhancement, Resonant raman, Retardation effects, Several advantages, Single mismatch, Ssdna, Surface plasmon absorption, Usual colorimetric technique, Verlag, Verlag gmbh, Viral sequence, Weinheim, Weinheim chem, Wide range, Widespread interest.
Abstract
Infectious diseases caused by the human immunodeficiency virus (HIV) remain the leading killers of human beings worldwide, and function to destabilize societies in Africa, Asia, and the Middle East. Driven by the need to detect the presence of HIV viral sequence, here we demonstrate that the second‐order nonlinear optical (NLO) properties of gold nanorods can be used for screening HIV‐1 viral DNA sequence without any modification, with good sensitivity (100 pico‐molar) and selectivity (single base‐pair mismatch). The hyper‐Rayleigh scattering (HRS) intensity increases 45 times when a label‐free 145‐mer, ss‐gag gene DNA, was hybridized with 100 pM target DNA. The mechanism of HRS intensity change has been discussed with experimental evidence for higher multipolar contribution to the NLO response of gold nanorods.
Gold picks out the gene: For the first time it has been demonstrated that nonlinear optical (NLO) properties of gold nanorods can be used for screening HIV‐1 gag gene DNA without any modification, and with excellent sensitivity (100 pM) and selectivity (single base‐pair mismatch; see figure).
Url:
DOI: 10.1002/chem.200701850
Affiliations:
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lett</term><term>Nanoparticles</term><term>Nanorod</term><term>Nanorods</term><term>Paper figure</term><term>Phys</term><term>Picomolar level</term><term>Pure gold nanorod solution</term><term>Raman</term><term>Resonance enhancement</term><term>Resonant raman</term><term>Retardation effects</term><term>Several advantages</term><term>Single mismatch</term><term>Ssdna</term><term>Surface plasmon absorption</term><term>Usual colorimetric technique</term><term>Verlag</term><term>Verlag gmbh</term><term>Viral sequence</term><term>Weinheim</term><term>Weinheim chem</term><term>Wide range</term><term>Widespread interest</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Infectious diseases caused by the human immunodeficiency virus (HIV) remain the leading killers of human beings worldwide, and function to destabilize societies in Africa, Asia, and the Middle East. Driven by the need to detect the presence of HIV viral sequence, here we demonstrate that the second‐order nonlinear optical (NLO) properties of gold nanorods can be used for screening HIV‐1 viral DNA sequence without any modification, with good sensitivity (100 pico‐molar) and selectivity (single base‐pair mismatch). The hyper‐Rayleigh scattering (HRS) intensity increases 45 times when a label‐free 145‐mer, ss‐gag gene DNA, was hybridized with 100 pM target DNA. The mechanism of HRS intensity change has been discussed with experimental evidence for higher multipolar contribution to the NLO response of gold nanorods.</div><div type="abstract" xml:lang="en">Gold picks out the gene: For the first time it has been demonstrated that nonlinear optical (NLO) properties of gold nanorods can be used for screening HIV‐1 gag gene DNA without any modification, and with excellent sensitivity (100 pM) and selectivity (single base‐pair mismatch; see figure).</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Darbha, Gopala Rishna" sort="Darbha, Gopala Rishna" uniqKey="Darbha G" first="Gopala Rishna" last="Darbha">Gopala Rishna Darbha</name><name sortKey="Rai, Uma Shanker" sort="Rai, Uma Shanker" uniqKey="Rai U" first="Uma Shanker" last="Rai">Uma Shanker Rai</name><name sortKey="Singh, Anant Umar" sort="Singh, Anant Umar" uniqKey="Singh A" first="Anant Umar" last="Singh">Anant Umar Singh</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Ray, Paresh Handra" sort="Ray, Paresh Handra" uniqKey="Ray P" first="Paresh Handra" last="Ray">Paresh Handra Ray</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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