Induction of immunity in sheep to Fasciola hepatica with mimotopes of cathepsin L selected from a phage display library.
Identifieur interne : 002974 ( Main/Exploration ); précédent : 002973; suivant : 002975Induction of immunity in sheep to Fasciola hepatica with mimotopes of cathepsin L selected from a phage display library.
Auteurs : A. Villa-Mancera ; H. Quiroz-Romero ; D. Correa ; F. Ibarra ; M. Reyes-Pérez ; H. Reyes-Vivas ; G. L Pez-Velázquez ; K. Gazarian ; T. Gazarian ; R A AlonsoSource :
- Parasitology [ 1469-8161 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Banque de peptides, Cathepsine L, Cathepsines (), Cathepsines (immunologie), Cysteine endopeptidases (), Cysteine endopeptidases (immunologie), Données de séquences moléculaires, Fasciola hepatica, Fasciolase (), Fasciolase (médecine vétérinaire), Fèces (parasitologie), Maladies des ovins (immunologie), Maladies des ovins (parasitologie), Numération des oeufs de parasites, Ovis, Séquence d'acides aminés, Vaccins (immunologie).
- MESH :
- immunologie : Cathepsines, Cysteine endopeptidases, Maladies des ovins, Vaccins.
- médecine vétérinaire : Fasciolase.
- parasitologie : Fèces, Maladies des ovins.
- Animaux, Banque de peptides, Cathepsine L, Cathepsines, Cysteine endopeptidases, Données de séquences moléculaires, Fasciola hepatica, Fasciolase, Numération des oeufs de parasites, Ovis, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Cathepsin L, Cathepsins (chemistry), Cathepsins (immunology), Cysteine Endopeptidases (chemistry), Cysteine Endopeptidases (immunology), Fasciola hepatica, Fascioliasis (prevention & control), Fascioliasis (veterinary), Feces (parasitology), Molecular Sequence Data, Parasite Egg Count, Peptide Library, Sheep, Sheep Diseases (immunology), Sheep Diseases (parasitology), Vaccines (immunology).
- MESH :
- chemical , chemistry : Cathepsins, Cysteine Endopeptidases.
- chemical , immunology : Cathepsins, Cysteine Endopeptidases, Vaccines.
- chemical : Cathepsin L, Peptide Library.
- immunology : Sheep Diseases.
- parasitology : Feces, Sheep Diseases.
- prevention & control : Fascioliasis.
- veterinary : Fascioliasis.
- Amino Acid Sequence, Animals, Fasciola hepatica, Molecular Sequence Data, Parasite Egg Count, Sheep.
Abstract
An M13 phage random 12-mers peptide library was used to screen cathepsin L mimotopes of Fasciola hepatica and to evaluate their immunogenicity in sheep. Seven clones showed positive reactivity to a rabbit anti-cathepsin L1/L2 antiserum in ELISA, and their amino acid sequences deduced by DNA sequencing were tentatively mapped on the protein. Twenty sheep were randomly allocated into 4 groups of 5 animals each, for immunization with 1x10(14) phage particles of clones 1, 20, a mixture of 7 clones and PBS, without adjuvant at the beginning, and 4 weeks later. All groups were challenged with 300 metacercariae at week 6 and slaughtered 16 weeks later. The mean worm burdens after challenge were reduced by 47.61% and 33.91% in sheep vaccinated with clones 1 and 20, respectively; no effect was observed in animals inoculated with the clone mixture. Also, a significant reduction in worm size and burden was observed for those sheep immunized with clone 1. Animals receiving clone 20, showed a significant reduction in egg output. Immunization induced a reduction of egg viability ranging from 58.92 to 82.11%. Furthermore, vaccinated animals produced clone-specific antibodies which were boosted after challenge with metacercariae of F. hepatica.
DOI: 10.1017/S003118200800471X
PubMed: 18812010
Affiliations:
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Ibarra</name></author><author><name sortKey="Reyes Perez, M" sort="Reyes Perez, M" uniqKey="Reyes Perez M" first="M" last="Reyes-Pérez">M. Reyes-Pérez</name></author><author><name sortKey="Reyes Vivas, H" sort="Reyes Vivas, H" uniqKey="Reyes Vivas H" first="H" last="Reyes-Vivas">H. Reyes-Vivas</name></author><author><name sortKey="L Pez Velazquez, G" sort="L Pez Velazquez, G" uniqKey="L Pez Velazquez G" first="G" last="L Pez-Velázquez">G. L Pez-Velázquez</name></author><author><name sortKey="Gazarian, K" sort="Gazarian, K" uniqKey="Gazarian K" first="K" last="Gazarian">K. Gazarian</name></author><author><name sortKey="Gazarian, T" sort="Gazarian, T" uniqKey="Gazarian T" first="T" last="Gazarian">T. 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Villa-Mancera</name><affiliation><nlm:affiliation>Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, México D.F. 04510.</nlm:affiliation><wicri:noCountry code="subField">México D.F.</wicri:noCountry></affiliation></author><author><name sortKey="Quiroz Romero, H" sort="Quiroz Romero, H" uniqKey="Quiroz Romero H" first="H" last="Quiroz-Romero">H. Quiroz-Romero</name></author><author><name sortKey="Correa, D" sort="Correa, D" uniqKey="Correa D" first="D" last="Correa">D. Correa</name></author><author><name sortKey="Ibarra, F" sort="Ibarra, F" uniqKey="Ibarra F" first="F" last="Ibarra">F. Ibarra</name></author><author><name sortKey="Reyes Perez, M" sort="Reyes Perez, M" uniqKey="Reyes Perez M" first="M" last="Reyes-Pérez">M. Reyes-Pérez</name></author><author><name sortKey="Reyes Vivas, H" sort="Reyes Vivas, H" uniqKey="Reyes Vivas H" first="H" last="Reyes-Vivas">H. Reyes-Vivas</name></author><author><name sortKey="L Pez Velazquez, G" sort="L Pez Velazquez, G" uniqKey="L Pez Velazquez G" first="G" last="L Pez-Velázquez">G. L Pez-Velázquez</name></author><author><name sortKey="Gazarian, K" sort="Gazarian, K" uniqKey="Gazarian K" first="K" last="Gazarian">K. Gazarian</name></author><author><name sortKey="Gazarian, T" sort="Gazarian, T" uniqKey="Gazarian T" first="T" last="Gazarian">T. Gazarian</name></author><author><name sortKey="Alonso, R A" sort="Alonso, R A" uniqKey="Alonso R" first="R A" last="Alonso">R A Alonso</name></author></analytic><series><title level="j">Parasitology</title><idno type="eISSN">1469-8161</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cathepsin L</term><term>Cathepsins (chemistry)</term><term>Cathepsins (immunology)</term><term>Cysteine Endopeptidases (chemistry)</term><term>Cysteine Endopeptidases (immunology)</term><term>Fasciola hepatica</term><term>Fascioliasis (prevention & control)</term><term>Fascioliasis (veterinary)</term><term>Feces (parasitology)</term><term>Molecular Sequence Data</term><term>Parasite Egg Count</term><term>Peptide Library</term><term>Sheep</term><term>Sheep Diseases (immunology)</term><term>Sheep Diseases (parasitology)</term><term>Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Banque de peptides</term><term>Cathepsine L</term><term>Cathepsines ()</term><term>Cathepsines (immunologie)</term><term>Cysteine endopeptidases ()</term><term>Cysteine endopeptidases (immunologie)</term><term>Données de séquences moléculaires</term><term>Fasciola hepatica</term><term>Fasciolase ()</term><term>Fasciolase (médecine vétérinaire)</term><term>Fèces (parasitologie)</term><term>Maladies des ovins (immunologie)</term><term>Maladies des ovins (parasitologie)</term><term>Numération des oeufs de parasites</term><term>Ovis</term><term>Séquence d'acides aminés</term><term>Vaccins (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cathepsins</term><term>Cysteine Endopeptidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Cathepsins</term><term>Cysteine Endopeptidases</term><term>Vaccines</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Cathepsin L</term><term>Peptide Library</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cathepsines</term><term>Cysteine endopeptidases</term><term>Maladies des ovins</term><term>Vaccins</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Sheep Diseases</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Fasciolase</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Fèces</term><term>Maladies des ovins</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Feces</term><term>Sheep Diseases</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Fascioliasis</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Fascioliasis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Fasciola hepatica</term><term>Molecular Sequence Data</term><term>Parasite Egg Count</term><term>Sheep</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Banque de peptides</term><term>Cathepsine L</term><term>Cathepsines</term><term>Cysteine endopeptidases</term><term>Données de séquences moléculaires</term><term>Fasciola hepatica</term><term>Fasciolase</term><term>Numération des oeufs de parasites</term><term>Ovis</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An M13 phage random 12-mers peptide library was used to screen cathepsin L mimotopes of Fasciola hepatica and to evaluate their immunogenicity in sheep. Seven clones showed positive reactivity to a rabbit anti-cathepsin L1/L2 antiserum in ELISA, and their amino acid sequences deduced by DNA sequencing were tentatively mapped on the protein. Twenty sheep were randomly allocated into 4 groups of 5 animals each, for immunization with 1x10(14) phage particles of clones 1, 20, a mixture of 7 clones and PBS, without adjuvant at the beginning, and 4 weeks later. All groups were challenged with 300 metacercariae at week 6 and slaughtered 16 weeks later. The mean worm burdens after challenge were reduced by 47.61% and 33.91% in sheep vaccinated with clones 1 and 20, respectively; no effect was observed in animals inoculated with the clone mixture. Also, a significant reduction in worm size and burden was observed for those sheep immunized with clone 1. Animals receiving clone 20, showed a significant reduction in egg output. Immunization induced a reduction of egg viability ranging from 58.92 to 82.11%. Furthermore, vaccinated animals produced clone-specific antibodies which were boosted after challenge with metacercariae of F. hepatica.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Alonso, R A" sort="Alonso, R A" uniqKey="Alonso R" first="R A" last="Alonso">R A Alonso</name><name sortKey="Correa, D" sort="Correa, D" uniqKey="Correa D" first="D" last="Correa">D. Correa</name><name sortKey="Gazarian, K" sort="Gazarian, K" uniqKey="Gazarian K" first="K" last="Gazarian">K. Gazarian</name><name sortKey="Gazarian, T" sort="Gazarian, T" uniqKey="Gazarian T" first="T" last="Gazarian">T. Gazarian</name><name sortKey="Ibarra, F" sort="Ibarra, F" uniqKey="Ibarra F" first="F" last="Ibarra">F. Ibarra</name><name sortKey="L Pez Velazquez, G" sort="L Pez Velazquez, G" uniqKey="L Pez Velazquez G" first="G" last="L Pez-Velázquez">G. L Pez-Velázquez</name><name sortKey="Quiroz Romero, H" sort="Quiroz Romero, H" uniqKey="Quiroz Romero H" first="H" last="Quiroz-Romero">H. Quiroz-Romero</name><name sortKey="Reyes Perez, M" sort="Reyes Perez, M" uniqKey="Reyes Perez M" first="M" last="Reyes-Pérez">M. Reyes-Pérez</name><name sortKey="Reyes Vivas, H" sort="Reyes Vivas, H" uniqKey="Reyes Vivas H" first="H" last="Reyes-Vivas">H. Reyes-Vivas</name><name sortKey="Villa Mancera, A" sort="Villa Mancera, A" uniqKey="Villa Mancera A" first="A" last="Villa-Mancera">A. Villa-Mancera</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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