Specific mutations within the AT-rich region of a plasmid replication origin affect either origin opening or helicase loading.
Identifieur interne : 002950 ( Main/Exploration ); précédent : 002949; suivant : 002951Specific mutations within the AT-rich region of a plasmid replication origin affect either origin opening or helicase loading.
Auteurs : Magdalena Rajewska [Pologne] ; Lukasz Kowalczyk ; Grazyna Konopa ; Igor KoniecznySource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2008.
Descripteurs français
- KwdFr :
- DnaB Helicases (génétique), DnaB Helicases (métabolisme), Escherichia coli (enzymologie), Escherichia coli (génétique), Mutation, Origine de réplication (physiologie), Plasmides (biosynthèse), Plasmides (génétique), Protéines Escherichia coli (génétique), Protéines Escherichia coli (métabolisme), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéines de liaison à l'ADN (génétique), Protéines de liaison à l'ADN (métabolisme), Réplication de l'ADN (physiologie), Séquence riche en AT (physiologie).
- MESH :
- biosynthèse : Plasmides.
- enzymologie : Escherichia coli.
- génétique : DnaB Helicases, Escherichia coli, Plasmides, Protéines Escherichia coli, Protéines bactériennes, Protéines de liaison à l'ADN.
- métabolisme : DnaB Helicases, Protéines Escherichia coli, Protéines bactériennes, Protéines de liaison à l'ADN.
- physiologie : Origine de réplication, Réplication de l'ADN, Séquence riche en AT.
- Mutation.
English descriptors
- KwdEn :
- AT Rich Sequence (physiology), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), DNA Replication (physiology), DNA-Binding Proteins (genetics), DNA-Binding Proteins (metabolism), DnaB Helicases (genetics), DnaB Helicases (metabolism), Escherichia coli (enzymology), Escherichia coli (genetics), Escherichia coli Proteins (genetics), Escherichia coli Proteins (metabolism), Mutation, Plasmids (biosynthesis), Plasmids (genetics), Replication Origin (physiology).
- MESH :
- chemical , genetics : Bacterial Proteins, DNA-Binding Proteins, DnaB Helicases, Escherichia coli Proteins.
- chemical , metabolism : Bacterial Proteins, DNA-Binding Proteins, DnaB Helicases, Escherichia coli Proteins.
- biosynthesis : Plasmids.
- enzymology : Escherichia coli.
- genetics : Escherichia coli, Plasmids.
- physiology : AT Rich Sequence, DNA Replication, Replication Origin.
- Mutation.
Abstract
Prokaryotic and eukaryotic replicons possess a distinctive region containing a higher than average number of adenine and thymine residues (the AT-rich region) where, during the process of replication initiation, the initial destabilization (opening) of the double helix takes place. In many prokaryotic origins, this region consists of repeated 13-mer motifs whose function has not yet been specified. Here we identify specific mutations within the 13-mer sequences of the broad-host-range plasmid RK2 that can result in defective origin opening or that do not affect opening but induce defects in helicase loading. We also show that after the initial recruitment of helicase at the DnaA-box sequences of the plasmid origin, the helicase is translocated to the AT-rich region in a reaction requiring specific sequence of the 13-mers and appropriate facing of the origin motifs. Our results demonstrate that specific sequences within the AT-rich region of a replication origin are required for either origin opening or helicase loading.
DOI: 10.1073/pnas.0805662105
PubMed: 18685104
Affiliations:
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Le document en format XML
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<term>DNA-Binding Proteins (genetics)</term>
<term>DNA-Binding Proteins (metabolism)</term>
<term>DnaB Helicases (genetics)</term>
<term>DnaB Helicases (metabolism)</term>
<term>Escherichia coli (enzymology)</term>
<term>Escherichia coli (genetics)</term>
<term>Escherichia coli Proteins (genetics)</term>
<term>Escherichia coli Proteins (metabolism)</term>
<term>Mutation</term>
<term>Plasmids (biosynthesis)</term>
<term>Plasmids (genetics)</term>
<term>Replication Origin (physiology)</term>
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<term>DnaB Helicases (métabolisme)</term>
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<term>Escherichia coli (génétique)</term>
<term>Mutation</term>
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<term>Plasmides (biosynthèse)</term>
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<term>Protéines de liaison à l'ADN (métabolisme)</term>
<term>Réplication de l'ADN (physiologie)</term>
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<term>Protéines bactériennes</term>
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<term>Protéines bactériennes</term>
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<front><div type="abstract" xml:lang="en">Prokaryotic and eukaryotic replicons possess a distinctive region containing a higher than average number of adenine and thymine residues (the AT-rich region) where, during the process of replication initiation, the initial destabilization (opening) of the double helix takes place. In many prokaryotic origins, this region consists of repeated 13-mer motifs whose function has not yet been specified. Here we identify specific mutations within the 13-mer sequences of the broad-host-range plasmid RK2 that can result in defective origin opening or that do not affect opening but induce defects in helicase loading. We also show that after the initial recruitment of helicase at the DnaA-box sequences of the plasmid origin, the helicase is translocated to the AT-rich region in a reaction requiring specific sequence of the 13-mers and appropriate facing of the origin motifs. Our results demonstrate that specific sequences within the AT-rich region of a replication origin are required for either origin opening or helicase loading.</div>
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