MersV1, Main, Exploration, bibRecord, 002808

Controlling the translocation of single-stranded DNA through alpha-hemolysin ion channels using viscosity.

Identifieur interne : 002808 ( Main/Exploration ); précédent : 002807; suivant : 002809

Controlling the translocation of single-stranded DNA through alpha-hemolysin ion channels using viscosity.

Auteurs : Ryuji Kawano [États-Unis] ; Anna E P. Schibel ; Christopher Cauley ; Henry S. White

Source :

Langmuir : the ACS journal of surfaces and colloids [ 0743-7463 ] ; 2009.

RBID : pubmed:19138164

Descripteurs français

KwdFr :
- ADN simple brin (), Canaux ioniques (), Chlorure de potassium (), Double couche lipidique (), Eau (), Glycérol (), Hémolysines (), Membrane artificielle, Polynucléotides (), Porosité, Propriétés de surface, Transition de phase, Verre (), Viscosité.
MESH :
- ADN simple brin, Canaux ioniques, Chlorure de potassium, Double couche lipidique, Eau, Glycérol, Hémolysines, Membrane artificielle, Polynucléotides, Porosité, Propriétés de surface, Transition de phase, Verre, Viscosité.

English descriptors

KwdEn :
- DNA, Single-Stranded (chemistry), Glass (chemistry), Glycerol (chemistry), Hemolysin Proteins (chemistry), Ion Channels (chemistry), Lipid Bilayers (chemistry), Membranes, Artificial, Phase Transition, Polynucleotides (chemistry), Porosity, Potassium Chloride (chemistry), Surface Properties, Viscosity, Water (chemistry).
MESH :
- chemical , chemistry : DNA, Single-Stranded, Glycerol, Hemolysin Proteins, Ion Channels, Lipid Bilayers, Polynucleotides, Potassium Chloride, Water.
- chemistry : Glass.
- chemical : Membranes, Artificial, Phase Transition, Porosity, Surface Properties, Viscosity.

Abstract

Translocation of single-stranded DNA through alpha-hemolysin (alpha-HL) channels is investigated in glycerol/water mixtures containing 1 M KCl. Experiments using glass nanopore membranes as the lipid bilayer support demonstrate that the translocation velocities of poly(deoxyadenylic acid), poly(deoxycytidylic acid), and poly(deoxythymidylic acid) 50-mers are decreased by a factor of approximately 20 in a 63/37 (vol %) glycerol/water mixture, relative to aqueous solutions. The ion conductance of alpha-HL and the entry rate of the polynucleotides into the protein channel also decrease with increasing viscosity. Precise control of translocation parameters by adjusting viscosity provides a potential means to improve sequencing methods based on ion channel recordings.

DOI: 10.1021/la803556p
PubMed: 19138164

Affiliations:

États-Unis

Le document en format XML

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<affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Utah, 315S. 1400E, Salt Lake City, Utah 84112, USA. rjkawano@chem.utah.edu</nlm:affiliation>
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<term>Glass (chemistry)</term>
<term>Glycerol (chemistry)</term>
<term>Hemolysin Proteins (chemistry)</term>
<term>Ion Channels (chemistry)</term>
<term>Lipid Bilayers (chemistry)</term>
<term>Membranes, Artificial</term>
<term>Phase Transition</term>
<term>Polynucleotides (chemistry)</term>
<term>Porosity</term>
<term>Potassium Chloride (chemistry)</term>
<term>Surface Properties</term>
<term>Viscosity</term>
<term>Water (chemistry)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN simple brin ()</term>
<term>Canaux ioniques ()</term>
<term>Chlorure de potassium ()</term>
<term>Double couche lipidique ()</term>
<term>Eau ()</term>
<term>Glycérol ()</term>
<term>Hémolysines ()</term>
<term>Membrane artificielle</term>
<term>Polynucléotides ()</term>
<term>Porosité</term>
<term>Propriétés de surface</term>
<term>Transition de phase</term>
<term>Verre ()</term>
<term>Viscosité</term>
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<term>Hemolysin Proteins</term>
<term>Ion Channels</term>
<term>Lipid Bilayers</term>
<term>Polynucleotides</term>
<term>Potassium Chloride</term>
<term>Water</term>
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<term>Phase Transition</term>
<term>Porosity</term>
<term>Surface Properties</term>
<term>Viscosity</term>
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<term>Eau</term>
<term>Glycérol</term>
<term>Hémolysines</term>
<term>Membrane artificielle</term>
<term>Polynucléotides</term>
<term>Porosité</term>
<term>Propriétés de surface</term>
<term>Transition de phase</term>
<term>Verre</term>
<term>Viscosité</term>
</keywords>
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</profileDesc>
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<front><div type="abstract" xml:lang="en">Translocation of single-stranded DNA through alpha-hemolysin (alpha-HL) channels is investigated in glycerol/water mixtures containing 1 M KCl. Experiments using glass nanopore membranes as the lipid bilayer support demonstrate that the translocation velocities of poly(deoxyadenylic acid), poly(deoxycytidylic acid), and poly(deoxythymidylic acid) 50-mers are decreased by a factor of approximately 20 in a 63/37 (vol %) glycerol/water mixture, relative to aqueous solutions. The ion conductance of alpha-HL and the entry rate of the polynucleotides into the protein channel also decrease with increasing viscosity. Precise control of translocation parameters by adjusting viscosity provides a potential means to improve sequencing methods based on ion channel recordings.</div>
</front>
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<name sortKey="Schibel, Anna E P" sort="Schibel, Anna E P" uniqKey="Schibel A" first="Anna E P" last="Schibel">Anna E P. Schibel</name>
<name sortKey="White, Henry S" sort="White, Henry S" uniqKey="White H" first="Henry S" last="White">Henry S. White</name>
</noCountry>
<country name="États-Unis"><noRegion><name sortKey="Kawano, Ryuji" sort="Kawano, Ryuji" uniqKey="Kawano R" first="Ryuji" last="Kawano">Ryuji Kawano</name>
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Serveur d'exploration MERS

Controlling the translocation of single-stranded DNA through alpha-hemolysin ion channels using viscosity.

Controlling the translocation of single-stranded DNA through alpha-hemolysin ion channels using viscosity.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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