Metal ion CHElate-aSSisted LIGAtion (CHESS LIGA) for SNP detection on microarrays.
Identifieur interne : 002783 ( Main/Exploration ); précédent : 002782; suivant : 002784Metal ion CHElate-aSSisted LIGAtion (CHESS LIGA) for SNP detection on microarrays.
Auteurs : Thi Hien Le [Russie] ; Tatiana S. Oretskaya ; Timofei S. ZatsepinSource :
- Bioorganic & medicinal chemistry letters [ 1464-3405 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN (), Chélateurs (), Données de séquences moléculaires, Humains, Ions (), Métaux (), Nickel (), Oligonucléotides, Polymorphisme de nucléotide simple, Procédures d'analyse sur micropuce (), Solubilité, Sondes d'ADN (), Séquence nucléotidique, Séquençage par oligonucléotides en batterie, Tétrahydrofolates ().
- MESH :
English descriptors
- KwdEn :
- Base Sequence, Chelating Agents (chemistry), DNA (chemistry), DNA Probes (chemistry), Humans, Ions (chemistry), Metals (chemistry), Microchip Analytical Procedures (methods), Molecular Sequence Data, Nickel (chemistry), Oligonucleotide Array Sequence Analysis, Oligonucleotides, Polymorphism, Single Nucleotide, Solubility, Tetrahydrofolates (chemistry).
- MESH :
- chemical , chemistry : Chelating Agents, DNA, DNA Probes, Ions, Metals, Nickel, Tetrahydrofolates.
- methods : Microchip Analytical Procedures.
- Base Sequence, Humans, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Oligonucleotides, Polymorphism, Single Nucleotide, Solubility.
Abstract
We developed a metal ion chelate-assisted ligation for SNP detection by microarray. An oligonucleotide probe was separated into two 9-10-mers bearing iminodiacetic residues at the gap point. Duplex formation with the DNA target was possible only if nickel ions were added, but a nucleotide substitution opposite the gap point prevented duplex formation. Here we demonstrate the application of this approach for SNP detection (A1298C) within the 5,10-methylenetetrahydrofolate reductase gene on a microarray.
DOI: 10.1016/j.bmcl.2009.06.033
PubMed: 19574044
Affiliations:
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Lomonossov Moscow State University, Leninskije Gory, Moscow 119991, Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Chemistry Department, M.V. Lomonossov Moscow State University, Leninskije Gory, Moscow 119991</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Oretskaya, Tatiana S" sort="Oretskaya, Tatiana S" uniqKey="Oretskaya T" first="Tatiana S" last="Oretskaya">Tatiana S. Oretskaya</name></author><author><name sortKey="Zatsepin, Timofei S" sort="Zatsepin, Timofei S" uniqKey="Zatsepin T" first="Timofei S" last="Zatsepin">Timofei S. Zatsepin</name></author></analytic><series><title level="j">Bioorganic & medicinal chemistry letters</title><idno type="eISSN">1464-3405</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Chelating Agents (chemistry)</term><term>DNA (chemistry)</term><term>DNA Probes (chemistry)</term><term>Humans</term><term>Ions (chemistry)</term><term>Metals (chemistry)</term><term>Microchip Analytical Procedures (methods)</term><term>Molecular Sequence Data</term><term>Nickel (chemistry)</term><term>Oligonucleotide Array Sequence Analysis</term><term>Oligonucleotides</term><term>Polymorphism, Single Nucleotide</term><term>Solubility</term><term>Tetrahydrofolates (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term><term>Chélateurs ()</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Ions ()</term><term>Métaux ()</term><term>Nickel ()</term><term>Oligonucléotides</term><term>Polymorphisme de nucléotide simple</term><term>Procédures d'analyse sur micropuce ()</term><term>Solubilité</term><term>Sondes d'ADN ()</term><term>Séquence nucléotidique</term><term>Séquençage par oligonucléotides en batterie</term><term>Tétrahydrofolates ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chelating Agents</term><term>DNA</term><term>DNA Probes</term><term>Ions</term><term>Metals</term><term>Nickel</term><term>Tetrahydrofolates</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microchip Analytical Procedures</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Humans</term><term>Molecular Sequence Data</term><term>Oligonucleotide Array Sequence Analysis</term><term>Oligonucleotides</term><term>Polymorphism, Single Nucleotide</term><term>Solubility</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>Chélateurs</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Ions</term><term>Métaux</term><term>Nickel</term><term>Oligonucléotides</term><term>Polymorphisme de nucléotide simple</term><term>Procédures d'analyse sur micropuce</term><term>Solubilité</term><term>Sondes d'ADN</term><term>Séquence nucléotidique</term><term>Séquençage par oligonucléotides en batterie</term><term>Tétrahydrofolates</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We developed a metal ion chelate-assisted ligation for SNP detection by microarray. An oligonucleotide probe was separated into two 9-10-mers bearing iminodiacetic residues at the gap point. Duplex formation with the DNA target was possible only if nickel ions were added, but a nucleotide substitution opposite the gap point prevented duplex formation. Here we demonstrate the application of this approach for SNP detection (A1298C) within the 5,10-methylenetetrahydrofolate reductase gene on a microarray.</div></front></TEI><affiliations><list><country><li>Russie</li></country><region><li>District fédéral central</li></region><settlement><li>Moscou</li></settlement></list><tree><noCountry><name sortKey="Oretskaya, Tatiana S" sort="Oretskaya, Tatiana S" uniqKey="Oretskaya T" first="Tatiana S" last="Oretskaya">Tatiana S. Oretskaya</name><name sortKey="Zatsepin, Timofei S" sort="Zatsepin, Timofei S" uniqKey="Zatsepin T" first="Timofei S" last="Zatsepin">Timofei S. Zatsepin</name></noCountry><country name="Russie"><region name="District fédéral central"><name sortKey="Le, Thi Hien" sort="Le, Thi Hien" uniqKey="Le T" first="Thi Hien" last="Le">Thi Hien Le</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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