Cyclic αvβ6‐targeting peptide selected from biopanning with clinical potential for head and neck squamous cell carcinoma
Identifieur interne : 002732 ( Main/Exploration ); précédent : 002731; suivant : 002733Cyclic αvβ6‐targeting peptide selected from biopanning with clinical potential for head and neck squamous cell carcinoma
Auteurs : Jenn-Ren Hsiao [Taïwan] ; Yao Chang [Taïwan] ; Yuh-Ling Chen [Taïwan] ; Su-Huei Hsieh [Taïwan] ; Keng-Fu Hsu [Taïwan] ; Chun-Fu Wang [Taïwan] ; Sen-Tien Tsai [Taïwan] ; Ying-Tai Jin [Taïwan]Source :
- Head & Neck [ 1043-3074 ] ; 2010-02.
English descriptors
- Teeft :
- Assay, Avb1, Avb3, Avb5, Avb6, Avb6 integrin, Biol, Biol chem, Biopanning, Cancer cells, Carcinoma, Cell carcinoma, Cell carcinomas, Chem, Clone, Cold dmem, Cold paraformaldehyde, Confocal, Confocal microscopy, Crge peptide, Crgelasl peptides, Cyclic, Cyclic peptide, Cyclic peptides, Different concentrations, Disease virus, Dmem, Donkey, February, Gure, Gure legends, Head february, Higher concentration, Hnscc, Integrin, Integrin avb6, Integrins, Internalization, Invasion front, Ligand, Linear peptides, Linear rgdlasl, Major kinds, Molecular probes, National cheng kung university, Online issue, Oral cancer cells, Oral cavity, Oscc, Oscc cells, Paraformaldehyde, Peptide, Phage, Phage clones, Phage display, Phage internalization, Phage particles, Primary antibodies, Receptor, Rgdlasl, Rgelasl, Rgelasl peptides, Room temperature, Santa cruz, Secondary antibodies, Separate experiment, Stroma invasion, Tumor cells, Tumor nests, Various cell lines, Vector phage, Virol.
Abstract
Background.: A cyclic peptide‐displaying phage library was used for biopanning on oral squamous cell carcinoma (OSCC) cells to identify cancer‐targeting peptides. This study was designed to characterize the receptor specificity of a candidate phage clone/peptide (phage/peptide‐29) and to explore the clinical potential of this peptide. Methods.: Immunofluorescent confocal microscopy, phage binding assay, and immunohistochemical studies were used to demonstrate the receptor specificity of phage/peptide‐29. The effect of peptide‐29 on the proliferation of OSCC cells was studied using 3‐dimensional (3D) cell cultures. Results.: Phage/peptide‐29 preferentially binds integrin αvβ6 rather than other αv‐associated integrins. Peptide‐29 significantly inhibits the proliferation of OSCC cells in 3D cell cultures. On human pathological sections, phage‐29 targets oral cancer cells in a αvβ6‐dependent manner. Besides, we showed that integrin αvβ6 is universally (94.7%, 36/38) expressed in all major kinds of head and neck squamous cell carcinomas (HNSCC). Conclusions.: Peptide‐29 selected from biopanning may have clinical potential for HNSCC. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Url:
DOI: 10.1002/hed.21166
Affiliations:
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Clinical Research, National Health Research Institutes, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yuh Ing" sort="Chen, Yuh Ing" uniqKey="Chen Y" first="Yuh-Ling" last="Chen">Yuh-Ling Chen</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Hsieh, Su Uei" sort="Hsieh, Su Uei" uniqKey="Hsieh S" first="Su-Huei" last="Hsieh">Su-Huei Hsieh</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Oral Medicine, College of Medicine, National Cheng Kung University, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Hsu, Keng U" sort="Hsu, Keng U" uniqKey="Hsu K" first="Keng-Fu" last="Hsu">Keng-Fu Hsu</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Obstetrics and Gynecology, National Cheng Kung University Medical College and Hospital, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Chun U" sort="Wang, Chun U" uniqKey="Wang C" first="Chun-Fu" last="Wang">Chun-Fu Wang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Biomedical Sciences, National Sun Yat‐Sen University, Kaohsiung</wicri:regionArea><wicri:noRegion>Kaohsiung</wicri:noRegion></affiliation></author><author><name sortKey="Tsai, Sen Ien" sort="Tsai, Sen Ien" uniqKey="Tsai S" first="Sen-Tien" last="Tsai">Sen-Tien Tsai</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Otolaryngology, National Cheng Kung University Medical College and Hospital, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Ying Ai" sort="Jin, Ying Ai" uniqKey="Jin Y" first="Ying-Tai" last="Jin">Ying-Tai Jin</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Pathology, Taiwan Adventis Hospital, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Taïwan</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Correspondence address: Department of Pathology, Taiwan Adventis Hospital, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Head & Neck</title><title level="j" type="alt">HEAD AND NECK</title><idno type="ISSN">1043-3074</idno><idno type="eISSN">1097-0347</idno><imprint><biblScope unit="vol">32</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="160">160</biblScope><biblScope unit="page" to="172">172</biblScope><biblScope unit="page-count">13</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2010-02">2010-02</date></imprint><idno type="ISSN">1043-3074</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1043-3074</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Avb1</term><term>Avb3</term><term>Avb5</term><term>Avb6</term><term>Avb6 integrin</term><term>Biol</term><term>Biol chem</term><term>Biopanning</term><term>Cancer cells</term><term>Carcinoma</term><term>Cell carcinoma</term><term>Cell carcinomas</term><term>Chem</term><term>Clone</term><term>Cold dmem</term><term>Cold paraformaldehyde</term><term>Confocal</term><term>Confocal microscopy</term><term>Crge peptide</term><term>Crgelasl peptides</term><term>Cyclic</term><term>Cyclic peptide</term><term>Cyclic peptides</term><term>Different concentrations</term><term>Disease virus</term><term>Dmem</term><term>Donkey</term><term>February</term><term>Gure</term><term>Gure legends</term><term>Head february</term><term>Higher concentration</term><term>Hnscc</term><term>Integrin</term><term>Integrin avb6</term><term>Integrins</term><term>Internalization</term><term>Invasion front</term><term>Ligand</term><term>Linear peptides</term><term>Linear rgdlasl</term><term>Major kinds</term><term>Molecular probes</term><term>National cheng kung university</term><term>Online issue</term><term>Oral cancer cells</term><term>Oral cavity</term><term>Oscc</term><term>Oscc cells</term><term>Paraformaldehyde</term><term>Peptide</term><term>Phage</term><term>Phage clones</term><term>Phage display</term><term>Phage internalization</term><term>Phage particles</term><term>Primary antibodies</term><term>Receptor</term><term>Rgdlasl</term><term>Rgelasl</term><term>Rgelasl peptides</term><term>Room temperature</term><term>Santa cruz</term><term>Secondary antibodies</term><term>Separate experiment</term><term>Stroma invasion</term><term>Tumor cells</term><term>Tumor nests</term><term>Various cell lines</term><term>Vector phage</term><term>Virol</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background.: A cyclic peptide‐displaying phage library was used for biopanning on oral squamous cell carcinoma (OSCC) cells to identify cancer‐targeting peptides. This study was designed to characterize the receptor specificity of a candidate phage clone/peptide (phage/peptide‐29) and to explore the clinical potential of this peptide. Methods.: Immunofluorescent confocal microscopy, phage binding assay, and immunohistochemical studies were used to demonstrate the receptor specificity of phage/peptide‐29. The effect of peptide‐29 on the proliferation of OSCC cells was studied using 3‐dimensional (3D) cell cultures. Results.: Phage/peptide‐29 preferentially binds integrin αvβ6 rather than other αv‐associated integrins. Peptide‐29 significantly inhibits the proliferation of OSCC cells in 3D cell cultures. On human pathological sections, phage‐29 targets oral cancer cells in a αvβ6‐dependent manner. Besides, we showed that integrin αvβ6 is universally (94.7%, 36/38) expressed in all major kinds of head and neck squamous cell carcinomas (HNSCC). Conclusions.: Peptide‐29 selected from biopanning may have clinical potential for HNSCC. © 2009 Wiley Periodicals, Inc. Head Neck, 2010</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Hsiao, Jenn En" sort="Hsiao, Jenn En" uniqKey="Hsiao J" first="Jenn-Ren" last="Hsiao">Jenn-Ren Hsiao</name></noRegion><name sortKey="Chang, Yao" sort="Chang, Yao" uniqKey="Chang Y" first="Yao" last="Chang">Yao Chang</name><name sortKey="Chen, Yuh Ing" sort="Chen, Yuh Ing" uniqKey="Chen Y" first="Yuh-Ling" last="Chen">Yuh-Ling Chen</name><name sortKey="Hsieh, Su Uei" sort="Hsieh, Su Uei" uniqKey="Hsieh S" first="Su-Huei" last="Hsieh">Su-Huei Hsieh</name><name sortKey="Hsu, Keng U" sort="Hsu, Keng U" uniqKey="Hsu K" first="Keng-Fu" last="Hsu">Keng-Fu Hsu</name><name sortKey="Jin, Ying Ai" sort="Jin, Ying Ai" uniqKey="Jin Y" first="Ying-Tai" last="Jin">Ying-Tai Jin</name><name sortKey="Jin, Ying Ai" sort="Jin, Ying Ai" uniqKey="Jin Y" first="Ying-Tai" last="Jin">Ying-Tai Jin</name><name sortKey="Jin, Ying Ai" sort="Jin, Ying Ai" uniqKey="Jin Y" first="Ying-Tai" last="Jin">Ying-Tai Jin</name><name sortKey="Tsai, Sen Ien" sort="Tsai, Sen Ien" uniqKey="Tsai S" first="Sen-Tien" last="Tsai">Sen-Tien Tsai</name><name sortKey="Wang, Chun U" sort="Wang, Chun U" uniqKey="Wang C" first="Chun-Fu" last="Wang">Chun-Fu Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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