Effects of biocides on gene expression in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough
Identifieur interne : 002725 ( Main/Exploration ); précédent : 002724; suivant : 002726Effects of biocides on gene expression in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough
Auteurs : Meng-Hsin Phoebe Lee [Canada] ; Sean M. Caffrey [Canada] ; Johanna K. Voordouw [Canada] ; Gerrit Voordouw [Canada]Source :
- Applied Microbiology and Biotechnology [ 0175-7598 ] ; 2010-07-01.
English descriptors
- KwdEn :
Abstract
Abstract: Although sulfate-reducing bacteria (SRB), such as Desulfovibrio vulgaris Hildenborough (DvH) are often eradicated in oil and gas operations with biocides, such as glutaraldehyde (Glut), tetrakis (hydroxymethyl) phosphonium sulfate (THPS), and benzalkonium chloride (BAC), their response to these agents is not well known. Whole genome microarrays of D. vulgaris treated with biocides well below the minimum inhibitory concentration showed that 256, 96, and 198 genes were responsive to Glut, THPS, and BAC, respectively, and that these three commonly used biocides affect the physiology of the cell quite differently. Glut induces expression of genes required to degrade or refold proteins inactivated by either chemical modification or heat shock, whereas BAC appears to target ribosomal structure. THPS appears to primarily affect energy metabolism of SRB. Mutants constructed for genes strongly up-regulated by Glut, were killed by Glut to a similar degree as the wild type. Hence, it is difficult to achieve increased sensitivity to this biocide by single gene mutations, because Glut affects so many targets. Our results increase understanding of the biocide's mode of action, allowing a more intelligent combination of mechanistically different agents. This can reduce stress on budgets for chemicals and on the environment.
Url:
DOI: 10.1007/s00253-010-2596-1
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001093
- to stream Istex, to step Curation: 001093
- to stream Istex, to step Checkpoint: 000596
- to stream Main, to step Merge: 002750
- to stream Main, to step Curation: 002725
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of biocides on gene expression in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough</title><author><name sortKey="Lee, Meng Hsin Phoebe" sort="Lee, Meng Hsin Phoebe" uniqKey="Lee M" first="Meng-Hsin Phoebe" last="Lee">Meng-Hsin Phoebe Lee</name></author><author><name sortKey="Caffrey, Sean M" sort="Caffrey, Sean M" uniqKey="Caffrey S" first="Sean M." last="Caffrey">Sean M. Caffrey</name></author><author><name sortKey="Voordouw, Johanna K" sort="Voordouw, Johanna K" uniqKey="Voordouw J" first="Johanna K." last="Voordouw">Johanna K. Voordouw</name></author><author><name sortKey="Voordouw, Gerrit" sort="Voordouw, Gerrit" uniqKey="Voordouw G" first="Gerrit" last="Voordouw">Gerrit Voordouw</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:933F99BDFFCE4570A7199EAD9BCCCAB17656BC46</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1007/s00253-010-2596-1</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-6RZCFW34-C/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001093</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001093</idno><idno type="wicri:Area/Istex/Curation">001093</idno><idno type="wicri:Area/Istex/Checkpoint">000596</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000596</idno><idno type="wicri:doubleKey">0175-7598:2010:Lee M:effects:of:biocides</idno><idno type="wicri:Area/Main/Merge">002750</idno><idno type="wicri:Area/Main/Curation">002725</idno><idno type="wicri:Area/Main/Exploration">002725</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Effects of biocides on gene expression in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough</title><author><name sortKey="Lee, Meng Hsin Phoebe" sort="Lee, Meng Hsin Phoebe" uniqKey="Lee M" first="Meng-Hsin Phoebe" last="Lee">Meng-Hsin Phoebe Lee</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Petroleum Microbiology Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, AB T2N 1N4, Calgary</wicri:regionArea><orgName type="university">Université de Calgary</orgName><placeName><settlement type="city">Calgary</settlement><region type="state">Alberta</region></placeName></affiliation></author><author><name sortKey="Caffrey, Sean M" sort="Caffrey, Sean M" uniqKey="Caffrey S" first="Sean M." last="Caffrey">Sean M. Caffrey</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Petroleum Microbiology Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, AB T2N 1N4, Calgary</wicri:regionArea><orgName type="university">Université de Calgary</orgName><placeName><settlement type="city">Calgary</settlement><region type="state">Alberta</region></placeName></affiliation></author><author><name sortKey="Voordouw, Johanna K" sort="Voordouw, Johanna K" uniqKey="Voordouw J" first="Johanna K." last="Voordouw">Johanna K. Voordouw</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Petroleum Microbiology Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, AB T2N 1N4, Calgary</wicri:regionArea><orgName type="university">Université de Calgary</orgName><placeName><settlement type="city">Calgary</settlement><region type="state">Alberta</region></placeName></affiliation></author><author><name sortKey="Voordouw, Gerrit" sort="Voordouw, Gerrit" uniqKey="Voordouw G" first="Gerrit" last="Voordouw">Gerrit Voordouw</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Petroleum Microbiology Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, AB T2N 1N4, Calgary</wicri:regionArea><orgName type="university">Université de Calgary</orgName><placeName><settlement type="city">Calgary</settlement><region type="state">Alberta</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Canada</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Applied Microbiology and Biotechnology</title><title level="j" type="abbrev">Appl Microbiol Biotechnol</title><idno type="ISSN">0175-7598</idno><idno type="eISSN">1432-0614</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2010-07-01">2010-07-01</date><biblScope unit="volume">87</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="1109">1109</biblScope><biblScope unit="page" to="1118">1118</biblScope></imprint><idno type="ISSN">0175-7598</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-7598</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biocide</term><term>Gene expression</term><term>Microarray</term><term>SRB</term><term>Souring</term><term>Sulfate</term><term>Sulfide</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Although sulfate-reducing bacteria (SRB), such as Desulfovibrio vulgaris Hildenborough (DvH) are often eradicated in oil and gas operations with biocides, such as glutaraldehyde (Glut), tetrakis (hydroxymethyl) phosphonium sulfate (THPS), and benzalkonium chloride (BAC), their response to these agents is not well known. Whole genome microarrays of D. vulgaris treated with biocides well below the minimum inhibitory concentration showed that 256, 96, and 198 genes were responsive to Glut, THPS, and BAC, respectively, and that these three commonly used biocides affect the physiology of the cell quite differently. Glut induces expression of genes required to degrade or refold proteins inactivated by either chemical modification or heat shock, whereas BAC appears to target ribosomal structure. THPS appears to primarily affect energy metabolism of SRB. Mutants constructed for genes strongly up-regulated by Glut, were killed by Glut to a similar degree as the wild type. Hence, it is difficult to achieve increased sensitivity to this biocide by single gene mutations, because Glut affects so many targets. Our results increase understanding of the biocide's mode of action, allowing a more intelligent combination of mechanistically different agents. This can reduce stress on budgets for chemicals and on the environment.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Alberta</li></region><settlement><li>Calgary</li></settlement><orgName><li>Université de Calgary</li></orgName></list><tree><country name="Canada"><region name="Alberta"><name sortKey="Lee, Meng Hsin Phoebe" sort="Lee, Meng Hsin Phoebe" uniqKey="Lee M" first="Meng-Hsin Phoebe" last="Lee">Meng-Hsin Phoebe Lee</name></region><name sortKey="Caffrey, Sean M" sort="Caffrey, Sean M" uniqKey="Caffrey S" first="Sean M." last="Caffrey">Sean M. Caffrey</name><name sortKey="Voordouw, Gerrit" sort="Voordouw, Gerrit" uniqKey="Voordouw G" first="Gerrit" last="Voordouw">Gerrit Voordouw</name><name sortKey="Voordouw, Gerrit" sort="Voordouw, Gerrit" uniqKey="Voordouw G" first="Gerrit" last="Voordouw">Gerrit Voordouw</name><name sortKey="Voordouw, Johanna K" sort="Voordouw, Johanna K" uniqKey="Voordouw J" first="Johanna K." last="Voordouw">Johanna K. Voordouw</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002725 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002725 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:933F99BDFFCE4570A7199EAD9BCCCAB17656BC46
|texte= Effects of biocides on gene expression in the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough
}}
| This area was generated with Dilib version V0.6.33. |  |