Cytotoxicity of lysomustine and its isomers, and their potential use for selection of cells
Identifieur interne : 002571 ( Main/Exploration ); précédent : 002570; suivant : 002572Cytotoxicity of lysomustine and its isomers, and their potential use for selection of cells
Auteurs : F. N. Rozov [Russie] ; T. S. Grinenko [Russie] ; G. L. Levit [Russie] ; A. N. Grishakov [Russie] ; A. V. Belyavsky [Russie] ; V. P. Krasnov [Russie]Source :
- Russian Journal of Bioorganic Chemistry [ 1068-1620 ] ; 2011-11-01.
English descriptors
- KwdEn :
Abstract
Abstract: N ɛ-Nitroso-N ɛ-[N′-(2-chloroethyl)carbamoyl]-L-lysine (I) and N ɛ-[N′-(2-chloroethyl)-N′-nitrosocarbamoyl]-L-lysine (II), the isomers being the constituents of antitumor agent lysomustine, were isolated from the agent by reverse phase HPLC (RP-HPLC). The study of cytotoxicity of the above compounds against K562 cells showed that lesions induced by isomer (II) produce a significant cytotoxic effect, but can be efficiently repaired by the action of MGMT (O6-methylguanine-DNA methyltransferase). Under similar conditions, lesions induced by isomer (I) produce a substantially smaller effect but are weakly, if at all, repairable by MGMT. The effects of a clinically approved agent lysomustine, which is a mixture of isomers (I) and (II), are similar to those of isomer (II). The results obtained point to a different chemical nature of DNA lesions induced by two lysomustine isomers. Our data indicate that lysomustine and its isomer (II) can be used for in vitro selection of cells expressing MGMT.
Url:
DOI: 10.1134/S1068162011060112
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000C71
- to stream Istex, to step Curation: 000C71
- to stream Istex, to step Checkpoint: 000500
- to stream Main, to step Merge: 002596
- to stream Main, to step Curation: 002571
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cytotoxicity of lysomustine and its isomers, and their potential use for selection of cells</title>
<author><name sortKey="Rozov, F N" sort="Rozov, F N" uniqKey="Rozov F" first="F. N." last="Rozov">F. N. Rozov</name>
</author>
<author><name sortKey="Grinenko, T S" sort="Grinenko, T S" uniqKey="Grinenko T" first="T. S." last="Grinenko">T. S. Grinenko</name>
</author>
<author><name sortKey="Levit, G L" sort="Levit, G L" uniqKey="Levit G" first="G. L." last="Levit">G. L. Levit</name>
</author>
<author><name sortKey="Grishakov, A N" sort="Grishakov, A N" uniqKey="Grishakov A" first="A. N." last="Grishakov">A. N. Grishakov</name>
</author>
<author><name sortKey="Belyavsky, A V" sort="Belyavsky, A V" uniqKey="Belyavsky A" first="A. V." last="Belyavsky">A. V. Belyavsky</name>
</author>
<author><name sortKey="Krasnov, V P" sort="Krasnov, V P" uniqKey="Krasnov V" first="V. P." last="Krasnov">V. P. Krasnov</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:8E9475CA937E7AE9200823D4BE50382D5475EF24</idno>
<date when="2011" year="2011">2011</date>
<idno type="doi">10.1134/S1068162011060112</idno>
<idno type="url">https://api.istex.fr/ark:/67375/VQC-P63BCL4L-5/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000C71</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C71</idno>
<idno type="wicri:Area/Istex/Curation">000C71</idno>
<idno type="wicri:Area/Istex/Checkpoint">000500</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000500</idno>
<idno type="wicri:doubleKey">1068-1620:2011:Rozov F:cytotoxicity:of:lysomustine</idno>
<idno type="wicri:Area/Main/Merge">002596</idno>
<idno type="wicri:Area/Main/Curation">002571</idno>
<idno type="wicri:Area/Main/Exploration">002571</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Cytotoxicity of lysomustine and its isomers, and their potential use for selection of cells</title>
<author><name sortKey="Rozov, F N" sort="Rozov, F N" uniqKey="Rozov F" first="F. N." last="Rozov">F. N. Rozov</name>
<affiliation wicri:level="3"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Grinenko, T S" sort="Grinenko, T S" uniqKey="Grinenko T" first="T. S." last="Grinenko">T. S. Grinenko</name>
<affiliation wicri:level="3"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Levit, G L" sort="Levit, G L" uniqKey="Levit G" first="G. L." last="Levit">G. L. Levit</name>
<affiliation wicri:level="1"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Postovsky Institute of Organic Synthesis, Ural Branch, Russian Academy of Sciences, ul. S. Kovalevskoy/Akademicheskaya, 22/20, 620990, Yekaterinburg</wicri:regionArea>
<wicri:noRegion>Yekaterinburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Grishakov, A N" sort="Grishakov, A N" uniqKey="Grishakov A" first="A. N." last="Grishakov">A. N. Grishakov</name>
<affiliation wicri:level="1"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Postovsky Institute of Organic Synthesis, Ural Branch, Russian Academy of Sciences, ul. S. Kovalevskoy/Akademicheskaya, 22/20, 620990, Yekaterinburg</wicri:regionArea>
<wicri:noRegion>Yekaterinburg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Belyavsky, A V" sort="Belyavsky, A V" uniqKey="Belyavsky A" first="A. V." last="Belyavsky">A. V. Belyavsky</name>
<affiliation wicri:level="3"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Krasnov, V P" sort="Krasnov, V P" uniqKey="Krasnov V" first="V. P." last="Krasnov">V. P. Krasnov</name>
<affiliation wicri:level="1"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Postovsky Institute of Organic Synthesis, Ural Branch, Russian Academy of Sciences, ul. S. Kovalevskoy/Akademicheskaya, 22/20, 620990, Yekaterinburg</wicri:regionArea>
<wicri:noRegion>Yekaterinburg</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Russie</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Russian Journal of Bioorganic Chemistry</title>
<title level="j" type="abbrev">Russ J Bioorg Chem</title>
<idno type="ISSN">1068-1620</idno>
<idno type="eISSN">1608-330X</idno>
<imprint><publisher>SP MAIK Nauka/Interperiodica</publisher>
<pubPlace>Dordrecht</pubPlace>
<date type="published" when="2011-11-01">2011-11-01</date>
<biblScope unit="volume">37</biblScope>
<biblScope unit="issue">6</biblScope>
<biblScope unit="page" from="713">713</biblScope>
<biblScope unit="page" to="718">718</biblScope>
</imprint>
<idno type="ISSN">1068-1620</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1068-1620</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>O6-benzylguanine</term>
<term>O6-methylguanine-DNA-methyltransferase</term>
<term>gene therapy</term>
<term>isomers</term>
<term>lysomustine</term>
<term>nitrosoureas</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: N ɛ-Nitroso-N ɛ-[N′-(2-chloroethyl)carbamoyl]-L-lysine (I) and N ɛ-[N′-(2-chloroethyl)-N′-nitrosocarbamoyl]-L-lysine (II), the isomers being the constituents of antitumor agent lysomustine, were isolated from the agent by reverse phase HPLC (RP-HPLC). The study of cytotoxicity of the above compounds against K562 cells showed that lesions induced by isomer (II) produce a significant cytotoxic effect, but can be efficiently repaired by the action of MGMT (O6-methylguanine-DNA methyltransferase). Under similar conditions, lesions induced by isomer (I) produce a substantially smaller effect but are weakly, if at all, repairable by MGMT. The effects of a clinically approved agent lysomustine, which is a mixture of isomers (I) and (II), are similar to those of isomer (II). The results obtained point to a different chemical nature of DNA lesions induced by two lysomustine isomers. Our data indicate that lysomustine and its isomer (II) can be used for in vitro selection of cells expressing MGMT.</div>
</front>
</TEI>
<affiliations><list><country><li>Russie</li>
</country>
<region><li>District fédéral central</li>
</region>
<settlement><li>Moscou</li>
</settlement>
</list>
<tree><country name="Russie"><region name="District fédéral central"><name sortKey="Rozov, F N" sort="Rozov, F N" uniqKey="Rozov F" first="F. N." last="Rozov">F. N. Rozov</name>
</region>
<name sortKey="Belyavsky, A V" sort="Belyavsky, A V" uniqKey="Belyavsky A" first="A. V." last="Belyavsky">A. V. Belyavsky</name>
<name sortKey="Grinenko, T S" sort="Grinenko, T S" uniqKey="Grinenko T" first="T. S." last="Grinenko">T. S. Grinenko</name>
<name sortKey="Grishakov, A N" sort="Grishakov, A N" uniqKey="Grishakov A" first="A. N." last="Grishakov">A. N. Grishakov</name>
<name sortKey="Krasnov, V P" sort="Krasnov, V P" uniqKey="Krasnov V" first="V. P." last="Krasnov">V. P. Krasnov</name>
<name sortKey="Krasnov, V P" sort="Krasnov, V P" uniqKey="Krasnov V" first="V. P." last="Krasnov">V. P. Krasnov</name>
<name sortKey="Levit, G L" sort="Levit, G L" uniqKey="Levit G" first="G. L." last="Levit">G. L. Levit</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002571 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002571 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:8E9475CA937E7AE9200823D4BE50382D5475EF24 |texte= Cytotoxicity of lysomustine and its isomers, and their potential use for selection of cells }}
This area was generated with Dilib version V0.6.33. |