RNA synthesis by reverse direction process: phosphoramidites and high purity RNAs and introduction of ligands, chromophores, and modifications at 3'-end.
Identifieur interne : 002440 ( Main/Exploration ); précédent : 002439; suivant : 002441RNA synthesis by reverse direction process: phosphoramidites and high purity RNAs and introduction of ligands, chromophores, and modifications at 3'-end.
Auteurs : Suresh C. Srivastava [États-Unis] ; Divya Pandey ; Naveen P. Srivastava ; Satya P. BajpaiSource :
- Current protocols in nucleic acid chemistry [ 1934-9289 ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemical synthesis : RNA.
- chemical , chemistry : Oligoribonucleotides, Organophosphorus Compounds, Organosilicon Compounds.
- chemical : Ligands.
- Base Sequence, Chromatography, High Pressure Liquid, Molecular Structure, Sequence Analysis, RNA.
Abstract
We have synthesized and studied the coupling properties of 3'-DMT-5'-CE phosphoramidites. The coupling efficiency per step surpasses 99% in the reverse-direction synthesis methodology, leading to high-purity RNA in a large number of 20- to 21-mers and long-chain oligonucleotides. Our data show that 5'→3' direction synthesis has a distinct advantage compared to the conventional method. As a result, this method of RNA synthesis is expected to be a very useful and practical method of choice for therapeutic-grade RNA. The phosphoramidites, Rev-A-n-bz, Rev-C-n-bz, Rev-C-n-ac, Rev-G-n-ac, and Rev-rU are routinely produced with an HPLC purity of greater than 98% and (31)P NMR purity greater than 99.5%.
DOI: 10.1002/0471142700.nc0320s45
PubMed: 21638272
Affiliations:
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Srivastava</name><affiliation wicri:level="2"><nlm:affiliation>ChemGenes Corp., Wilmington, Massachusetts, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>ChemGenes Corp., Wilmington, Massachusetts</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Pandey, Divya" sort="Pandey, Divya" uniqKey="Pandey D" first="Divya" last="Pandey">Divya Pandey</name></author><author><name sortKey="Srivastava, Naveen P" sort="Srivastava, Naveen P" uniqKey="Srivastava N" first="Naveen P" last="Srivastava">Naveen P. Srivastava</name></author><author><name sortKey="Bajpai, Satya P" sort="Bajpai, Satya P" uniqKey="Bajpai S" first="Satya P" last="Bajpai">Satya P. 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The coupling efficiency per step surpasses 99% in the reverse-direction synthesis methodology, leading to high-purity RNA in a large number of 20- to 21-mers and long-chain oligonucleotides. Our data show that 5'→3' direction synthesis has a distinct advantage compared to the conventional method. As a result, this method of RNA synthesis is expected to be a very useful and practical method of choice for therapeutic-grade RNA. The phosphoramidites, Rev-A-n-bz, Rev-C-n-bz, Rev-C-n-ac, Rev-G-n-ac, and Rev-rU are routinely produced with an HPLC purity of greater than 98% and (31)P NMR purity greater than 99.5%.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><noCountry><name sortKey="Bajpai, Satya P" sort="Bajpai, Satya P" uniqKey="Bajpai S" first="Satya P" last="Bajpai">Satya P. Bajpai</name><name sortKey="Pandey, Divya" sort="Pandey, Divya" uniqKey="Pandey D" first="Divya" last="Pandey">Divya Pandey</name><name sortKey="Srivastava, Naveen P" sort="Srivastava, Naveen P" uniqKey="Srivastava N" first="Naveen P" last="Srivastava">Naveen P. Srivastava</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Srivastava, Suresh C" sort="Srivastava, Suresh C" uniqKey="Srivastava S" first="Suresh C" last="Srivastava">Suresh C. Srivastava</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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