MersV1, Main, Exploration, bibRecord, 002255

Fabrication of duplex DNA microarrays incorporating methyl-5-cytosine.

Identifieur interne : 002255 ( Main/Exploration ); précédent : 002254; suivant : 002256

Fabrication of duplex DNA microarrays incorporating methyl-5-cytosine.

Auteurs : Christopher L. Warren [États-Unis] ; Jianfei Zhao ; Kimberly Glass ; Vikas Rishi ; Aseem Z. Ansari ; Charles Vinson

Source :

Lab on a chip [ 1473-0189 ] ; 2012.

RBID : pubmed:22139143

Descripteurs français

KwdFr :
- 5-Méthyl-cytosine (immunologie), 5-Méthyl-cytosine (métabolisme), ADN (métabolisme), Anticorps (immunologie), Ilots CpG, Méthylation de l'ADN, Protéine de liaison à l'élément de réponse à l'AMP cyclique (génétique), Séquençage par oligonucléotides en batterie (instrumentation), Test de retard de migration électrophorétique (instrumentation).
MESH :
- génétique : Protéine de liaison à l'élément de réponse à l'AMP cyclique.
- immunologie : 5-Méthyl-cytosine, Anticorps.
- métabolisme : 5-Méthyl-cytosine, ADN, Séquençage par oligonucléotides en batterie, Test de retard de migration électrophorétique.
- Ilots CpG, Méthylation de l'ADN.

English descriptors

KwdEn :
- 5-Methylcytosine (immunology), 5-Methylcytosine (metabolism), Antibodies (immunology), CpG Islands, Cyclic AMP Response Element-Binding Protein (genetics), DNA (metabolism), DNA Methylation, Electrophoretic Mobility Shift Assay (instrumentation), Oligonucleotide Array Sequence Analysis (instrumentation).
MESH :
- chemical , genetics : Cyclic AMP Response Element-Binding Protein.
- chemical , immunology : 5-Methylcytosine, Antibodies.
- chemical , metabolism : 5-Methylcytosine, DNA.
- instrumentation : Electrophoretic Mobility Shift Assay, Oligonucleotide Array Sequence Analysis.
- CpG Islands, DNA Methylation.

Abstract

We synthesized customized double-stranded DNA microarrays including methyl-5-cytosine at CpG dinucleotides and produced all 163,555 possible 8-mers (un-, hemi-, and di-methylated) to gain insight into how methylation affects transcription factor binding. An antibody to methyl-5-cytidine showed greater binding to the methylated DNA, demonstrating efficient incorporation of methyl-5-cytosine into the synthesized DNA. In contrast, binding of the transcription factor CREB was inhibited by CpG methylation. This platform represents a powerful new technology to evaluate the effect of DNA methylation on protein binding in any sequence context.

DOI: 10.1039/c1lc20698b
PubMed: 22139143

Affiliations:

Le document en format XML

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<term>CpG Islands</term>
<term>Cyclic AMP Response Element-Binding Protein (genetics)</term>
<term>DNA (metabolism)</term>
<term>DNA Methylation</term>
<term>Electrophoretic Mobility Shift Assay (instrumentation)</term>
<term>Oligonucleotide Array Sequence Analysis (instrumentation)</term>
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<term>ADN (métabolisme)</term>
<term>Anticorps (immunologie)</term>
<term>Ilots CpG</term>
<term>Méthylation de l'ADN</term>
<term>Protéine de liaison à l'élément de réponse à l'AMP cyclique (génétique)</term>
<term>Séquençage par oligonucléotides en batterie (instrumentation)</term>
<term>Test de retard de migration électrophorétique (instrumentation)</term>
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<term>Séquençage par oligonucléotides en batterie</term>
<term>Test de retard de migration électrophorétique</term>
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<term>Méthylation de l'ADN</term>
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<front><div type="abstract" xml:lang="en">We synthesized customized double-stranded DNA microarrays including methyl-5-cytosine at CpG dinucleotides and produced all 163,555 possible 8-mers (un-, hemi-, and di-methylated) to gain insight into how methylation affects transcription factor binding. An antibody to methyl-5-cytidine showed greater binding to the methylated DNA, demonstrating efficient incorporation of methyl-5-cytosine into the synthesized DNA. In contrast, binding of the transcription factor CREB was inhibited by CpG methylation. This platform represents a powerful new technology to evaluate the effect of DNA methylation on protein binding in any sequence context.</div>
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<name sortKey="Vinson, Charles" sort="Vinson, Charles" uniqKey="Vinson C" first="Charles" last="Vinson">Charles Vinson</name>
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<country name="États-Unis"><region name="Wisconsin"><name sortKey="Warren, Christopher L" sort="Warren, Christopher L" uniqKey="Warren C" first="Christopher L" last="Warren">Christopher L. Warren</name>
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Serveur d'exploration MERS

Fabrication of duplex DNA microarrays incorporating methyl-5-cytosine.

Fabrication of duplex DNA microarrays incorporating methyl-5-cytosine.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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