Chloride and organic osmolytes: a hybrid strategy to cope with elevated salinities by the moderately halophilic, chloride‐dependent bacterium Halobacillus halophilus
Identifieur interne : 002154 ( Main/Exploration ); précédent : 002153; suivant : 002155Chloride and organic osmolytes: a hybrid strategy to cope with elevated salinities by the moderately halophilic, chloride‐dependent bacterium Halobacillus halophilus
Auteurs : Stephan H. Saum [Allemagne] ; Friedhelm Pfeiffer [Allemagne] ; Peter Palm [Allemagne] ; Markus Rampp ; Stephan C. Schuster [États-Unis] ; Volker Müller [Allemagne] ; Dieter Oesterhelt [Allemagne]Source :
- Environmental Microbiology [ 1462-2912 ] ; 2013-05.
English descriptors
- Teeft :
- Acidic, Alanine, Alanine dehydrogenase, Amino, Amino acids, Annotation, Arch microbiol, Aspartate, Aspartate family, Bacillaceae, Bacillus, Bacillus subtilis, Bacteriol, Bacterium, Betaine, Biol chem, Biosynthesis, Blackwell publishing, Catalysed, Chloride, Chloride dependence, Chromosome, Clone, Cog, Compatible solutes, Cosmid, Cosmid library, Data analysis, Dehydrogenase, Diguanylate, Ectoine, Encoding, Environ microbiol, Environmental microbiology, Escherichia coli, Family bacillaceae, Gene, Genome, Genome sequence, Germination, Glutamate, Glutamate family, Glutamine, Glycine, Glycine betaine, Halobacillus, Halobacillus halophilus, Halobacterium, Halophile, Halophilic, Halophilic bacteria, Halophilic bacterium, Halophilic organisms, Halophilus, Hydroxyectoine, Kinase, Lysine, Megan analysis, Microbiol, Microbiology, Moderate halophile, Moderate halophile halobacillus halophilus, Moderate halophiles, Molar concentrations, Nacl, Nucleic, Nucleic acids, Oceanobacillus, Oceanobacillus iheyensis, Operon, Ornithine, Osmolyte, Osmolyte strategy, Other organisms, Pathway, Pfeiffer, Plasmid, Proline, Protein, Proteome, Regulator, Replication, Rrna, Rrna operons, Salinity, Salt concentrations, Salt stress, Saum, Sequencing, Severin, Solute, Stationary growth phase, Subtilis, Transmembrane, Transmembrane domains, Transporter, Trna, Trna genes.
Abstract
Salt acclimation in moderately halophilic bacteria is the result of action of a grand interplay orchestrated by signals perceived from the environment. To elucidate the cellular players involved in sensing and responding to changing salinities we have determined the genome sequence of Halobacillus halophilus, a Gram‐positive moderate halophilic bacterium that has a strict requirement for the anion chloride. Halobacillus halophilus synthesizes a multitude of different compatible solutes and switches its osmolyte strategy with the external salinity and growth phase. Based on the emerging genome sequence, the compatible solutes glutamate, glutamine, proline and ectoine have already been experimentally studied. The biosynthetic routes for acetyl ornithine and acetyl lysine are also delineated from the genome sequence. Halobacillus halophilus is nutritionally very versatile and most compatible solutes cannot only be produced but also used as carbon and energy sources. The genome sequence unravelled isogenes for many pathways indicating a fine regulation of metabolism. Halobacillus halophilus is unique in integrating the concept of compatible solutes with the second fundamental principle to cope with salt stress, the accumulation of molar concentrations of salt (Cl‐) in the cytoplasm. Extremely halophilic bacteria/archaea, which exclusively rely on the salt‐in strategy, have a high percentage of acidic proteins compared with non‐halophiles with a low percentage. Halobacillus halophilus has an intermediate position which is consistent with its ability to integrate both principles.
Url:
DOI: 10.1111/j.1462-2920.2012.02770.x
Affiliations:
- Allemagne, États-Unis
- District de Darmstadt, Hesse (Land)
- Francfort-sur-le-Main
- Université Johann Wolfgang Goethe de Francfort-sur-le-Main
Links toward previous steps (curation, corpus...)
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- to stream Istex, to step Curation: 000B66
- to stream Istex, to step Checkpoint: 000292
- to stream Main, to step Merge: 002178
- to stream Main, to step Curation: 002154
Le document en format XML
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unit="issue">5</biblScope><biblScope unit="page" from="1619">1619</biblScope><biblScope unit="page" to="1633">1633</biblScope><biblScope unit="page-count">15</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2013-05">2013-05</date></imprint><idno type="ISSN">1462-2912</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1462-2912</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acidic</term><term>Alanine</term><term>Alanine dehydrogenase</term><term>Amino</term><term>Amino acids</term><term>Annotation</term><term>Arch microbiol</term><term>Aspartate</term><term>Aspartate family</term><term>Bacillaceae</term><term>Bacillus</term><term>Bacillus subtilis</term><term>Bacteriol</term><term>Bacterium</term><term>Betaine</term><term>Biol chem</term><term>Biosynthesis</term><term>Blackwell publishing</term><term>Catalysed</term><term>Chloride</term><term>Chloride dependence</term><term>Chromosome</term><term>Clone</term><term>Cog</term><term>Compatible solutes</term><term>Cosmid</term><term>Cosmid library</term><term>Data analysis</term><term>Dehydrogenase</term><term>Diguanylate</term><term>Ectoine</term><term>Encoding</term><term>Environ microbiol</term><term>Environmental microbiology</term><term>Escherichia coli</term><term>Family bacillaceae</term><term>Gene</term><term>Genome</term><term>Genome sequence</term><term>Germination</term><term>Glutamate</term><term>Glutamate family</term><term>Glutamine</term><term>Glycine</term><term>Glycine betaine</term><term>Halobacillus</term><term>Halobacillus halophilus</term><term>Halobacterium</term><term>Halophile</term><term>Halophilic</term><term>Halophilic bacteria</term><term>Halophilic bacterium</term><term>Halophilic organisms</term><term>Halophilus</term><term>Hydroxyectoine</term><term>Kinase</term><term>Lysine</term><term>Megan analysis</term><term>Microbiol</term><term>Microbiology</term><term>Moderate halophile</term><term>Moderate halophile halobacillus halophilus</term><term>Moderate halophiles</term><term>Molar concentrations</term><term>Nacl</term><term>Nucleic</term><term>Nucleic acids</term><term>Oceanobacillus</term><term>Oceanobacillus iheyensis</term><term>Operon</term><term>Ornithine</term><term>Osmolyte</term><term>Osmolyte strategy</term><term>Other organisms</term><term>Pathway</term><term>Pfeiffer</term><term>Plasmid</term><term>Proline</term><term>Protein</term><term>Proteome</term><term>Regulator</term><term>Replication</term><term>Rrna</term><term>Rrna operons</term><term>Salinity</term><term>Salt concentrations</term><term>Salt stress</term><term>Saum</term><term>Sequencing</term><term>Severin</term><term>Solute</term><term>Stationary growth phase</term><term>Subtilis</term><term>Transmembrane</term><term>Transmembrane domains</term><term>Transporter</term><term>Trna</term><term>Trna genes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Salt acclimation in moderately halophilic bacteria is the result of action of a grand interplay orchestrated by signals perceived from the environment. To elucidate the cellular players involved in sensing and responding to changing salinities we have determined the genome sequence of Halobacillus halophilus, a Gram‐positive moderate halophilic bacterium that has a strict requirement for the anion chloride. Halobacillus halophilus synthesizes a multitude of different compatible solutes and switches its osmolyte strategy with the external salinity and growth phase. Based on the emerging genome sequence, the compatible solutes glutamate, glutamine, proline and ectoine have already been experimentally studied. The biosynthetic routes for acetyl ornithine and acetyl lysine are also delineated from the genome sequence. Halobacillus halophilus is nutritionally very versatile and most compatible solutes cannot only be produced but also used as carbon and energy sources. The genome sequence unravelled isogenes for many pathways indicating a fine regulation of metabolism. Halobacillus halophilus is unique in integrating the concept of compatible solutes with the second fundamental principle to cope with salt stress, the accumulation of molar concentrations of salt (Cl‐) in the cytoplasm. Extremely halophilic bacteria/archaea, which exclusively rely on the salt‐in strategy, have a high percentage of acidic proteins compared with non‐halophiles with a low percentage. Halobacillus halophilus has an intermediate position which is consistent with its ability to integrate both principles.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>District de Darmstadt</li><li>Hesse (Land)</li></region><settlement><li>Francfort-sur-le-Main</li></settlement><orgName><li>Université Johann Wolfgang Goethe de Francfort-sur-le-Main</li></orgName></list><tree><noCountry><name sortKey="Rampp, Markus" sort="Rampp, Markus" uniqKey="Rampp M" first="Markus" last="Rampp">Markus Rampp</name></noCountry><country name="Allemagne"><region name="Hesse (Land)"><name sortKey="Saum, Stephan H" sort="Saum, Stephan H" uniqKey="Saum S" first="Stephan H." last="Saum">Stephan H. Saum</name></region><name sortKey="Muller, Volker" sort="Muller, Volker" uniqKey="Muller V" first="Volker" last="Müller">Volker Müller</name><name sortKey="Muller, Volker" sort="Muller, Volker" uniqKey="Muller V" first="Volker" last="Müller">Volker Müller</name><name sortKey="Oesterhelt, Dieter" sort="Oesterhelt, Dieter" uniqKey="Oesterhelt D" first="Dieter" last="Oesterhelt">Dieter Oesterhelt</name><name sortKey="Palm, Peter" sort="Palm, Peter" uniqKey="Palm P" first="Peter" last="Palm">Peter Palm</name><name sortKey="Pfeiffer, Friedhelm" sort="Pfeiffer, Friedhelm" uniqKey="Pfeiffer F" first="Friedhelm" last="Pfeiffer">Friedhelm Pfeiffer</name></country><country name="États-Unis"><noRegion><name sortKey="Schuster, Stephan C" sort="Schuster, Stephan C" uniqKey="Schuster S" first="Stephan C." last="Schuster">Stephan C. Schuster</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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