Poly‐L‐Arginine Grafted Silica Mesoporous Nanoparticles for Enhanced Cellular Uptake and their Application in DNA Delivery and Controlled Drug Release
Identifieur interne : 002118 ( Main/Exploration ); précédent : 002117; suivant : 002119Poly‐L‐Arginine Grafted Silica Mesoporous Nanoparticles for Enhanced Cellular Uptake and their Application in DNA Delivery and Controlled Drug Release
Auteurs : Mrityunjoy Kar [Inde] ; Neha Tiwari [Inde] ; Mitali Tiwari [Inde] ; Mayurika Lahiri [Inde] ; Sayam Sen Gupta [Inde]Source :
- Particle & Particle Systems Characterization [ 0934-0866 ] ; 2013-02.
Abstract
Mesoporous silica nanoparticles (MSNs), that are capable of delivering gene and drugs to organisms in an effective and selective way have attracted much attention lately for its potential in the treatment of cancer. However, the successful application of MSNs for delivery of plasmid DNA or drugs requires surface modification of the silica with positively charged functional groups so that it binds to the negatively charged nucleic acids and also helps it penetrate through the cell membrane. We report for the first time the synthesis of a hybrid MSN where the cell penetrating cationic polypeptide poly‐L‐arginine synthesized by NCA polymerization is grafted onto the external surface of MSN using click chemistry. These poly‐L‐arginine grafted MSNs show low cytotoxity (85% cell viability at 100 μg/mL MSN concentration) and high cellular uptake by both HeLa and A549 (>90%). The poly‐L‐arginine grafted MSNs were used effectively to deliver mCherry DNA plasmid into cells leading to expression of the protein mCherry inside the cells (transfection efficiency 60%). In contrast, poly‐L‐arginine grafted non‐porous silica nanoparticles were unable to express the protein mCherry inside the cells although their uptake into the cells was as efficient as with poly‐L‐arginine grafted MSNs. We also show preliminary results to demonstrate that these hybrid MSNs can be used as a delivery vehicle for the anticancer drug Doxorubicin towards cancerous cells HeLa and A549. The biocompatibility of poly‐L‐arginine and its cell penetrating ability are expected to make these MSN conjugates very useful carriers for the delivery of genes and drugs into cancer cells.
Poly‐L‐arginine grafted mesoporous silica nanoparticles have been shown to be an efficient carrier for the transfection of DNA plasmid and the delivery of anti‐cancer drug Doxorubicin into cancer cells.
Url:
DOI: 10.1002/ppsc.201200089
Affiliations:
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wicri:level="1"><country xml:lang="fr">Inde</country><wicri:regionArea>Indian Institute of Science Education and Research, Sutarwadi Road, Pashan, 411 021, Pune</wicri:regionArea><wicri:noRegion>Pune</wicri:noRegion></affiliation></author><author><name sortKey="Lahiri, Mayurika" sort="Lahiri, Mayurika" uniqKey="Lahiri M" first="Mayurika" last="Lahiri">Mayurika Lahiri</name><affiliation wicri:level="1"><country xml:lang="fr">Inde</country><wicri:regionArea>Indian Institute of Science Education and Research, Sutarwadi Road, Pashan, 411 021, Pune</wicri:regionArea><wicri:noRegion>Pune</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Inde</country></affiliation></author><author><name sortKey="Gupta, Sayam Sen" sort="Gupta, Sayam Sen" uniqKey="Gupta S" first="Sayam Sen" last="Gupta">Sayam Sen Gupta</name><affiliation wicri:level="1"><country xml:lang="fr">Inde</country><wicri:regionArea>Chemical engineering Division, National Chemical Laboratory, Dr. Homi Bhabha Road, 411008, Pune</wicri:regionArea><wicri:noRegion>Pune</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Inde</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Particle & Particle Systems Characterization</title><title level="j" type="alt">PARTICLE AND PARTICLE SYSTEMS CHARACTERIZATION</title><idno type="ISSN">0934-0866</idno><idno type="eISSN">1521-4117</idno><imprint><biblScope unit="vol">30</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="166">166</biblScope><biblScope unit="page" to="179">179</biblScope><biblScope unit="page-count">14</biblScope><date type="published" when="2013-02">2013-02</date></imprint><idno type="ISSN">0934-0866</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0934-0866</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mesoporous silica nanoparticles (MSNs), that are capable of delivering gene and drugs to organisms in an effective and selective way have attracted much attention lately for its potential in the treatment of cancer. However, the successful application of MSNs for delivery of plasmid DNA or drugs requires surface modification of the silica with positively charged functional groups so that it binds to the negatively charged nucleic acids and also helps it penetrate through the cell membrane. We report for the first time the synthesis of a hybrid MSN where the cell penetrating cationic polypeptide poly‐L‐arginine synthesized by NCA polymerization is grafted onto the external surface of MSN using click chemistry. These poly‐L‐arginine grafted MSNs show low cytotoxity (85% cell viability at 100 μg/mL MSN concentration) and high cellular uptake by both HeLa and A549 (>90%). The poly‐L‐arginine grafted MSNs were used effectively to deliver mCherry DNA plasmid into cells leading to expression of the protein mCherry inside the cells (transfection efficiency 60%). In contrast, poly‐L‐arginine grafted non‐porous silica nanoparticles were unable to express the protein mCherry inside the cells although their uptake into the cells was as efficient as with poly‐L‐arginine grafted MSNs. We also show preliminary results to demonstrate that these hybrid MSNs can be used as a delivery vehicle for the anticancer drug Doxorubicin towards cancerous cells HeLa and A549. The biocompatibility of poly‐L‐arginine and its cell penetrating ability are expected to make these MSN conjugates very useful carriers for the delivery of genes and drugs into cancer cells.</div><div type="abstract" xml:lang="en">Poly‐L‐arginine grafted mesoporous silica nanoparticles have been shown to be an efficient carrier for the transfection of DNA plasmid and the delivery of anti‐cancer drug Doxorubicin into cancer cells.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Kar, Mrityunjoy" sort="Kar, Mrityunjoy" uniqKey="Kar M" first="Mrityunjoy" last="Kar">Mrityunjoy Kar</name></noRegion><name sortKey="Gupta, Sayam Sen" sort="Gupta, Sayam Sen" uniqKey="Gupta S" first="Sayam Sen" last="Gupta">Sayam Sen Gupta</name><name sortKey="Gupta, Sayam Sen" sort="Gupta, Sayam Sen" uniqKey="Gupta S" first="Sayam Sen" last="Gupta">Sayam Sen Gupta</name><name sortKey="Lahiri, Mayurika" sort="Lahiri, Mayurika" uniqKey="Lahiri M" first="Mayurika" last="Lahiri">Mayurika Lahiri</name><name sortKey="Lahiri, Mayurika" sort="Lahiri, Mayurika" uniqKey="Lahiri M" first="Mayurika" last="Lahiri">Mayurika Lahiri</name><name sortKey="Tiwari, Mitali" sort="Tiwari, Mitali" uniqKey="Tiwari M" first="Mitali" last="Tiwari">Mitali Tiwari</name><name sortKey="Tiwari, Neha" sort="Tiwari, Neha" uniqKey="Tiwari N" first="Neha" last="Tiwari">Neha Tiwari</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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