MersV1, Main, Exploration, bibRecord, 001F83

High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography.

Identifieur interne : 001F83 ( Main/Exploration ); précédent : 001F82; suivant : 001F84

High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography.

Auteurs : Kecheng Li [République populaire de Chine] ; Song Liu ; Ronge Xing ; Huahua Yu ; Yukun Qin ; Rongfeng Li ; Pengcheng Li

Source :

Journal of separation science [ 1615-9314 ] ; 2013.

RBID : pubmed:23457118

Descripteurs français

English descriptors

KwdEn :
- Chitosan (chemistry), Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Oligosaccharides (chemistry), Spectrometry, Mass, Electrospray Ionization.
MESH :
- chemical , chemistry : Chitosan, Oligosaccharides.
- Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Spectrometry, Mass, Electrospray Ionization.

Abstract

Highly purified chitooligomers with single degree of polymerization are of significance for studying bioactivity of chitooligomers. However, there are few reports on high-resolution preparative separation of chitooligomers, especially for those oligomers with degree of polymerization higher than 4. This study developed a high-resolution chromatography for the preparative separation of a pure fully deacetylated chitooligomer series. A glucosamine oligomer mixture with low degree of polymerization was prepared by acid hydrolysis of a highly deacetylated chitosan. Then, six fractions were separated from the prepared oligomer mixture by ion-exchange chromatography and analyzed by HPLC and ESI/MS, which primarily contained glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively, with chromatographic purities over 98% for dimers to hexamers and a purity of 93% for heptamers. The yields of a single round of separation were 75, 60, 60, 55, 35, and 20 mg for glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively. Furthermore, a chromatographic separation model for GlcN homomers was established. The capacity factor (k) of glucosamine oligomers and their degrees of polymerization (DPs) exhibited a good correlation, lnk = 0.786 + 0.846 lnDP, (R(2) = 0.997). Based on this equation, glucosamine octamers are expected to be separated by this system.

DOI: 10.1002/jssc.201200935
PubMed: 23457118

Affiliations:

République populaire de Chine

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<front><div type="abstract" xml:lang="en">Highly purified chitooligomers with single degree of polymerization are of significance for studying bioactivity of chitooligomers. However, there are few reports on high-resolution preparative separation of chitooligomers, especially for those oligomers with degree of polymerization higher than 4. This study developed a high-resolution chromatography for the preparative separation of a pure fully deacetylated chitooligomer series. A glucosamine oligomer mixture with low degree of polymerization was prepared by acid hydrolysis of a highly deacetylated chitosan. Then, six fractions were separated from the prepared oligomer mixture by ion-exchange chromatography and analyzed by HPLC and ESI/MS, which primarily contained glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively, with chromatographic purities over 98% for dimers to hexamers and a purity of 93% for heptamers. The yields of a single round of separation were 75, 60, 60, 55, 35, and 20 mg for glucosamine dimers, trimers, tetramers, pentamers, hexamers, and heptamers, respectively. Furthermore, a chromatographic separation model for GlcN homomers was established. The capacity factor (k) of glucosamine oligomers and their degrees of polymerization (DPs) exhibited a good correlation, lnk = 0.786 + 0.846 lnDP, (R(2) = 0.997). Based on this equation, glucosamine octamers are expected to be separated by this system.</div>
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High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography.

High-resolution separation of homogeneous chitooligomers series from 2-mers to 7-mers by ion-exchange chromatography.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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