Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus.
Identifieur interne : 001B09 ( Main/Exploration ); précédent : 001B08; suivant : 001B10Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus.
Auteurs : Xiaoyan Lu [États-Unis] ; Brett Whitaker ; Senthil Kumar K. Sakthivel ; Shifaq Kamili ; Laura E. Rose ; Luis Lowe ; Emad Mohareb ; Emad M. Elassal ; Tarek Al-Sanouri ; Aktham Haddadin ; Dean D. ErdmanSource :
- Journal of clinical microbiology [ 1098-660X ] ; 2014.
Descripteurs français
- KwdFr :
- Adulte, Coronavirus (), Coronavirus (génétique), Coronavirus (isolement et purification), Food and Drug Administration (USA), Humains, Infections de l'appareil respiratoire (diagnostic), Infections de l'appareil respiratoire (virologie), Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Nourrisson, Nouveau-né, RT-PCR (), Réaction de polymérisation en chaine en temps réel (), Techniques de diagnostic moléculaire (), Trousses de réactifs pour diagnostic, États-Unis d'Amérique.
- MESH :
- diagnostic : Infections de l'appareil respiratoire, Infections à coronavirus.
- génétique : Coronavirus.
- isolement et purification : Coronavirus.
- virologie : Infections de l'appareil respiratoire, Infections à coronavirus.
- Adulte, Coronavirus, Food and Drug Administration (USA), Humains, Nourrisson, Nouveau-né, RT-PCR, Réaction de polymérisation en chaine en temps réel, Techniques de diagnostic moléculaire, Trousses de réactifs pour diagnostic, États-Unis d'Amérique.
- Wicri :
- geographic : États-Unis.
English descriptors
- KwdEn :
- Adult, Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Humans, Infant, Infant, Newborn, Molecular Diagnostic Techniques (methods), Reagent Kits, Diagnostic, Real-Time Polymerase Chain Reaction (methods), Respiratory Tract Infections (diagnosis), Respiratory Tract Infections (virology), Reverse Transcriptase Polymerase Chain Reaction (methods), United States, United States Food and Drug Administration.
- MESH :
- chemical : Reagent Kits, Diagnostic.
- geographic : United States.
- classification : Coronavirus.
- diagnosis : Coronavirus Infections, Respiratory Tract Infections.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Coronavirus Infections, Respiratory Tract Infections.
- Adult, Humans, Infant, Infant, Newborn, United States Food and Drug Administration.
Abstract
A new human coronavirus (CoV), subsequently named Middle East respiratory syndrome (MERS)-CoV, was first reported in Saudi Arabia in September 2012. In response, we developed two real-time reverse transcription-PCR (rRT-PCR) assays targeting the MERS-CoV nucleocapsid (N) gene and evaluated these assays as a panel with a previously published assay targeting the region upstream of the MERS-CoV envelope gene (upE) for the detection and confirmation of MERS-CoV infection. All assays detected ≤10 copies/reaction of quantified RNA transcripts, with a linear dynamic range of 8 log units and 1.3 × 10(-3) 50% tissue culture infective doses (TCID50)/ml of cultured MERS-CoV per reaction. All assays performed comparably with respiratory, serum, and stool specimens spiked with cultured virus. No false-positive amplifications were obtained with other human coronaviruses or common respiratory viral pathogens or with 336 diverse clinical specimens from non-MERS-CoV cases; specimens from two confirmed MERS-CoV cases were positive with all assay signatures. In June 2012, the U.S. Food and Drug Administration authorized emergency use of the rRT-PCR assay panel as an in vitro diagnostic test for MERS-CoV. A kit consisting of the three assay signatures and a positive control was assembled and distributed to public health laboratories in the United States and internationally to support MERS-CoV surveillance and public health responses.
DOI: 10.1128/JCM.02533-13
PubMed: 24153118
Affiliations:
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Erdman</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Coronavirus (classification)</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>Molecular Diagnostic Techniques (methods)</term><term>Reagent Kits, Diagnostic</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Respiratory Tract Infections (diagnosis)</term><term>Respiratory Tract Infections (virology)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>United States</term><term>United States Food and Drug Administration</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Coronavirus ()</term><term>Coronavirus (génétique)</term><term>Coronavirus (isolement et purification)</term><term>Food and Drug Administration (USA)</term><term>Humains</term><term>Infections de l'appareil respiratoire (diagnostic)</term><term>Infections de l'appareil respiratoire (virologie)</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Nourrisson</term><term>Nouveau-né</term><term>RT-PCR ()</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Techniques de diagnostic moléculaire ()</term><term>Trousses de réactifs pour diagnostic</term><term>États-Unis d'Amérique</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Reagent Kits, Diagnostic</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>United States</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections de l'appareil respiratoire</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term><term>Real-Time Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections de l'appareil respiratoire</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Humans</term><term>Infant</term><term>Infant, Newborn</term><term>United States Food and Drug Administration</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Coronavirus</term><term>Food and Drug Administration (USA)</term><term>Humains</term><term>Nourrisson</term><term>Nouveau-né</term><term>RT-PCR</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Techniques de diagnostic moléculaire</term><term>Trousses de réactifs pour diagnostic</term><term>États-Unis d'Amérique</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>États-Unis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new human coronavirus (CoV), subsequently named Middle East respiratory syndrome (MERS)-CoV, was first reported in Saudi Arabia in September 2012. In response, we developed two real-time reverse transcription-PCR (rRT-PCR) assays targeting the MERS-CoV nucleocapsid (N) gene and evaluated these assays as a panel with a previously published assay targeting the region upstream of the MERS-CoV envelope gene (upE) for the detection and confirmation of MERS-CoV infection. All assays detected ≤10 copies/reaction of quantified RNA transcripts, with a linear dynamic range of 8 log units and 1.3 × 10(-3) 50% tissue culture infective doses (TCID50)/ml of cultured MERS-CoV per reaction. All assays performed comparably with respiratory, serum, and stool specimens spiked with cultured virus. No false-positive amplifications were obtained with other human coronaviruses or common respiratory viral pathogens or with 336 diverse clinical specimens from non-MERS-CoV cases; specimens from two confirmed MERS-CoV cases were positive with all assay signatures. In June 2012, the U.S. Food and Drug Administration authorized emergency use of the rRT-PCR assay panel as an in vitro diagnostic test for MERS-CoV. A kit consisting of the three assay signatures and a positive control was assembled and distributed to public health laboratories in the United States and internationally to support MERS-CoV surveillance and public health responses. </div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><noCountry><name sortKey="Al Sanouri, Tarek" sort="Al Sanouri, Tarek" uniqKey="Al Sanouri T" first="Tarek" last="Al-Sanouri">Tarek Al-Sanouri</name><name sortKey="Elassal, Emad M" sort="Elassal, Emad M" uniqKey="Elassal E" first="Emad M" last="Elassal">Emad M. Elassal</name><name sortKey="Erdman, Dean D" sort="Erdman, Dean D" uniqKey="Erdman D" first="Dean D" last="Erdman">Dean D. Erdman</name><name sortKey="Haddadin, Aktham" sort="Haddadin, Aktham" uniqKey="Haddadin A" first="Aktham" last="Haddadin">Aktham Haddadin</name><name sortKey="Kamili, Shifaq" sort="Kamili, Shifaq" uniqKey="Kamili S" first="Shifaq" last="Kamili">Shifaq Kamili</name><name sortKey="Lowe, Luis" sort="Lowe, Luis" uniqKey="Lowe L" first="Luis" last="Lowe">Luis Lowe</name><name sortKey="Mohareb, Emad" sort="Mohareb, Emad" uniqKey="Mohareb E" first="Emad" last="Mohareb">Emad Mohareb</name><name sortKey="Rose, Laura E" sort="Rose, Laura E" uniqKey="Rose L" first="Laura E" last="Rose">Laura E. Rose</name><name sortKey="Sakthivel, Senthil Kumar K" sort="Sakthivel, Senthil Kumar K" uniqKey="Sakthivel S" first="Senthil Kumar K" last="Sakthivel">Senthil Kumar K. Sakthivel</name><name sortKey="Whitaker, Brett" sort="Whitaker, Brett" uniqKey="Whitaker B" first="Brett" last="Whitaker">Brett Whitaker</name></noCountry><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Lu, Xiaoyan" sort="Lu, Xiaoyan" uniqKey="Lu X" first="Xiaoyan" last="Lu">Xiaoyan Lu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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