Crystallographic analysis of the N‐terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein
Identifieur interne : 001994 ( Main/Exploration ); précédent : 001993; suivant : 001995Crystallographic analysis of the N‐terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein
Auteurs : Yong-Sheng Wang ; Chung-Ke Chang ; Ming-Hon HouSource :
- Acta Crystallographica Section F [ 2053-230X ] ; 2015-08-01.
Descripteurs français
- KwdFr :
- Clonage moléculaire, Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (métabolisme), Cristallisation, Cristallographie aux rayons X, Données de séquences moléculaires, Escherichia coli (génétique), Escherichia coli (métabolisme), Expression des gènes, Protéines de fusion recombinantes (), Protéines de fusion recombinantes (génétique), Protéines nucléocapside (), Protéines nucléocapside (génétique), Structure tertiaire des protéines, Séquence d'acides aminés.
- MESH :
- génétique : Escherichia coli, Protéines de fusion recombinantes, Protéines nucléocapside.
- métabolisme : Coronavirus du syndrome respiratoire du Moyen-Orient, Escherichia coli.
- Clonage moléculaire, Coronavirus du syndrome respiratoire du Moyen-Orient, Cristallisation, Cristallographie aux rayons X, Données de séquences moléculaires, Expression des gènes, Protéines de fusion recombinantes, Protéines nucléocapside, Structure tertiaire des protéines, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Cloning, Molecular, Crystallization, Crystallography, X-Ray, Escherichia coli (genetics), Escherichia coli (metabolism), Gene Expression, Middle East Respiratory Syndrome Coronavirus (chemistry), Middle East Respiratory Syndrome Coronavirus (metabolism), Molecular Sequence Data, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Protein Structure, Tertiary, Recombinant Fusion Proteins (chemistry), Recombinant Fusion Proteins (genetics).
- MESH :
- chemical , chemistry : Nucleocapsid Proteins, Recombinant Fusion Proteins.
- chemistry : Middle East Respiratory Syndrome Coronavirus.
- genetics : Escherichia coli, Nucleocapsid Proteins, Recombinant Fusion Proteins.
- metabolism : Escherichia coli, Middle East Respiratory Syndrome Coronavirus.
- Amino Acid Sequence, Cloning, Molecular, Crystallization, Crystallography, X-Ray, Gene Expression, Molecular Sequence Data, Protein Structure, Tertiary.
Abstract
The N‐terminal domain of the nucleocapsid protein from Middle East respiratory syndrome coronavirus (MERS‐CoV NP‐NTD) contains many positively charged residues and has been identified to be responsible for RNA binding during ribonucleocapsid formation by the virus. In this study, the crystallization and crystallographic analysis of MERS‐CoV NP‐NTD (amino acids 39–165), with a molecular weight of 14.7 kDa, are reported. MERS‐CoV NP‐NTD was crystallized at 293 K using PEG 3350 as a precipitant and a 94.5% complete native data set was collected from a cooled crystal at 77 K to 2.63 Å resolution with an overall Rmerge of 9.6%. The crystals were monoclinic and belonged to space group P21, with unit‐cell parameters a = 35.60, b = 109.64, c = 91.99 Å, β = 101.22°. The asymmetric unit contained four MERS‐CoV NP‐NTD molecules.
Url:
- https://api.istex.fr/ark:/67375/WNG-B7DGJ9PD-W/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528927
DOI: 10.1107/S2053230X15010146
Affiliations:
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Le document en format XML
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<term>Cloning, Molecular</term>
<term>Crystallization</term>
<term>Crystallography, X-Ray</term>
<term>Escherichia coli (genetics)</term>
<term>Escherichia coli (metabolism)</term>
<term>Gene Expression</term>
<term>Middle East Respiratory Syndrome Coronavirus (chemistry)</term>
<term>Middle East Respiratory Syndrome Coronavirus (metabolism)</term>
<term>Molecular Sequence Data</term>
<term>Nucleocapsid Proteins (chemistry)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Protein Structure, Tertiary</term>
<term>Recombinant Fusion Proteins (chemistry)</term>
<term>Recombinant Fusion Proteins (genetics)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Clonage moléculaire</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (métabolisme)</term>
<term>Cristallisation</term>
<term>Cristallographie aux rayons X</term>
<term>Données de séquences moléculaires</term>
<term>Escherichia coli (génétique)</term>
<term>Escherichia coli (métabolisme)</term>
<term>Expression des gènes</term>
<term>Protéines de fusion recombinantes ()</term>
<term>Protéines de fusion recombinantes (génétique)</term>
<term>Protéines nucléocapside ()</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Recombinant Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
<term>Nucleocapsid Proteins</term>
<term>Recombinant Fusion Proteins</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines nucléocapside</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term>
<term>Middle East Respiratory Syndrome Coronavirus</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Cloning, Molecular</term>
<term>Crystallization</term>
<term>Crystallography, X-Ray</term>
<term>Gene Expression</term>
<term>Molecular Sequence Data</term>
<term>Protein Structure, Tertiary</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Cristallisation</term>
<term>Cristallographie aux rayons X</term>
<term>Données de séquences moléculaires</term>
<term>Expression des gènes</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines nucléocapside</term>
<term>Structure tertiaire des protéines</term>
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<front><div type="abstract" xml:lang="en">The N‐terminal domain of the nucleocapsid protein from Middle East respiratory syndrome coronavirus (MERS‐CoV NP‐NTD) contains many positively charged residues and has been identified to be responsible for RNA binding during ribonucleocapsid formation by the virus. In this study, the crystallization and crystallographic analysis of MERS‐CoV NP‐NTD (amino acids 39–165), with a molecular weight of 14.7 kDa, are reported. MERS‐CoV NP‐NTD was crystallized at 293 K using PEG 3350 as a precipitant and a 94.5% complete native data set was collected from a cooled crystal at 77 K to 2.63 Å resolution with an overall Rmerge of 9.6%. The crystals were monoclinic and belonged to space group P21, with unit‐cell parameters a = 35.60, b = 109.64, c = 91.99 Å, β = 101.22°. The asymmetric unit contained four MERS‐CoV NP‐NTD molecules.</div>
</front>
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<name sortKey="Hou, Ming On" sort="Hou, Ming On" uniqKey="Hou M" first="Ming-Hon" last="Hou">Ming-Hon Hou</name>
<name sortKey="Wang, Yong Heng" sort="Wang, Yong Heng" uniqKey="Wang Y" first="Yong-Sheng" last="Wang">Yong-Sheng Wang</name>
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