The diagnostic potential of MPT63‐derived HLA‐A*0201‐restricted CD8+T‐cell epitopes for active pulmonary tuberculosis
Identifieur interne : 001948 ( Main/Exploration ); précédent : 001947; suivant : 001949The diagnostic potential of MPT63‐derived HLA‐A*0201‐restricted CD8+T‐cell epitopes for active pulmonary tuberculosis
Auteurs : Zhiliang Duan ; Dezhou Li ; Qingjun Jia ; Juanjuan Xu ; Xinyu Chen ; Zhigang Xu ; Huifang Liu ; Bokun Chen ; Jinsheng Wen [République populaire de Chine]Source :
- Microbiology and Immunology [ 0385-5600 ] ; 2015-12.
Abstract
MPT63 protein is found only in Mycobacterium tuberculosis complex, including M. tuberculosis and M. bovis. Detection of MPT63‐specific IFN‐γ‐secreting T cells could be useful for the diagnosis of tuberculosis (TB) diseases. In the present study, the HLA‐A*0201 restriction of ten predicted MPT63‐derived CD8+T‐cell epitopes was assessed on the basis of T2 cell line and HLA‐A*0201 transgenic mice. The diagnostic potential of immunogenic peptides in active pulmonary TB patients was evaluated using an IFN‐γ enzyme‐linked immunospot assay. It was found that five peptides bound to HLA‐A*0201 with high affinity, whereas the remaining peptides exhibited low affinity for HLA‐A*0201. Five immunogenic peptides (MPT6318–26, MPT6329–37, MPT6320–28, MPT635–14 and MPT6310–19) elicited large numbers of cytotoxic IFN‐γ‐secreting T cells in HLA‐A*0201 transgenic mice. Each of the five immunogenic peptides was recognized by peripheral blood mononuclear cells from 45% to 73% of 40 HLA‐A*0201 positive TB patients. The total diagnostic sensitivity of the five immunogenic peptides was higher than that of a T‐SPOT.TB assay (based on ESAT‐6 and CFP‐10) (93% versus 90%). It is noticeable that the diagnostic sensitivity of the combination of five immunogenic peptides and T‐SPOT.TB assay reached 100%. These MPT63‐derived HLA‐A*0201‐restricted CD8+T‐cell epitopes would likely contribute to the immunological diagnosis of M. tuberculosis infection and may provide the components for designing an effective TB vaccine.
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DOI: 10.1111/1348-0421.12339
Affiliations:
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first="Qingjun" last="Jia">Qingjun Jia</name><affiliation></affiliation><affiliation><wicri:noCountry code="subField">Chashan</wicri:noCountry></affiliation></author><author><name sortKey="Xu, Juanjuan" sort="Xu, Juanjuan" uniqKey="Xu J" first="Juanjuan" last="Xu">Juanjuan Xu</name><affiliation></affiliation><affiliation><wicri:noCountry code="subField">Chashan</wicri:noCountry></affiliation></author><author><name sortKey="Chen, Xinyu" sort="Chen, Xinyu" uniqKey="Chen X" first="Xinyu" last="Chen">Xinyu Chen</name><affiliation></affiliation><affiliation><wicri:noCountry code="subField">Chashan</wicri:noCountry></affiliation></author><author><name sortKey="Xu, Zhigang" sort="Xu, Zhigang" uniqKey="Xu Z" first="Zhigang" last="Xu">Zhigang Xu</name><affiliation></affiliation><affiliation><wicri:noCountry code="subField">Chashan</wicri:noCountry></affiliation></author><author><name sortKey="Liu, Huifang" sort="Liu, Huifang" uniqKey="Liu H" first="Huifang" last="Liu">Huifang 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from="705">705</biblScope><biblScope unit="page" to="715">715</biblScope><biblScope unit="page-count">11</biblScope><date type="published" when="2015-12">2015-12</date></imprint><idno type="ISSN">0385-5600</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0385-5600</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">MPT63 protein is found only in Mycobacterium tuberculosis complex, including M. tuberculosis and M. bovis. Detection of MPT63‐specific IFN‐γ‐secreting T cells could be useful for the diagnosis of tuberculosis (TB) diseases. In the present study, the HLA‐A*0201 restriction of ten predicted MPT63‐derived CD8+T‐cell epitopes was assessed on the basis of T2 cell line and HLA‐A*0201 transgenic mice. The diagnostic potential of immunogenic peptides in active pulmonary TB patients was evaluated using an IFN‐γ enzyme‐linked immunospot assay. It was found that five peptides bound to HLA‐A*0201 with high affinity, whereas the remaining peptides exhibited low affinity for HLA‐A*0201. Five immunogenic peptides (MPT6318–26, MPT6329–37, MPT6320–28, MPT635–14 and MPT6310–19) elicited large numbers of cytotoxic IFN‐γ‐secreting T cells in HLA‐A*0201 transgenic mice. Each of the five immunogenic peptides was recognized by peripheral blood mononuclear cells from 45% to 73% of 40 HLA‐A*0201 positive TB patients. The total diagnostic sensitivity of the five immunogenic peptides was higher than that of a T‐SPOT.TB assay (based on ESAT‐6 and CFP‐10) (93% versus 90%). It is noticeable that the diagnostic sensitivity of the combination of five immunogenic peptides and T‐SPOT.TB assay reached 100%. These MPT63‐derived HLA‐A*0201‐restricted CD8+T‐cell epitopes would likely contribute to the immunological diagnosis of M. tuberculosis infection and may provide the components for designing an effective TB vaccine.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Bokun" sort="Chen, Bokun" uniqKey="Chen B" first="Bokun" last="Chen">Bokun Chen</name><name sortKey="Chen, Xinyu" sort="Chen, Xinyu" uniqKey="Chen X" first="Xinyu" last="Chen">Xinyu Chen</name><name sortKey="Duan, Zhiliang" sort="Duan, Zhiliang" uniqKey="Duan Z" first="Zhiliang" last="Duan">Zhiliang Duan</name><name sortKey="Jia, Qingjun" sort="Jia, Qingjun" uniqKey="Jia Q" first="Qingjun" last="Jia">Qingjun Jia</name><name sortKey="Li, Dezhou" sort="Li, Dezhou" uniqKey="Li D" first="Dezhou" last="Li">Dezhou Li</name><name sortKey="Liu, Huifang" sort="Liu, Huifang" uniqKey="Liu H" first="Huifang" last="Liu">Huifang Liu</name><name sortKey="Xu, Juanjuan" sort="Xu, Juanjuan" uniqKey="Xu J" first="Juanjuan" last="Xu">Juanjuan Xu</name><name sortKey="Xu, Zhigang" sort="Xu, Zhigang" uniqKey="Xu Z" first="Zhigang" last="Xu">Zhigang Xu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wen, Jinsheng" sort="Wen, Jinsheng" uniqKey="Wen J" first="Jinsheng" last="Wen">Jinsheng Wen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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