Clinical validation of 3 commercial real-time reverse transcriptase polymerase chain reaction assays for the detection of Middle East respiratory syndrome coronavirus from upper respiratory tract specimens
Identifieur interne : 000E57 ( Main/Exploration ); précédent : 000E56; suivant : 000E58Clinical validation of 3 commercial real-time reverse transcriptase polymerase chain reaction assays for the detection of Middle East respiratory syndrome coronavirus from upper respiratory tract specimens
Auteurs : Deqa H. Mohamed [Arabie saoudite] ; Abdulkarim F. Alhetheel [Arabie saoudite] ; Hanat S. Mohamud [Arabie saoudite] ; Kamel Aldosari [Arabie saoudite] ; Fahad A. Alzamil [Arabie saoudite] ; Ali M. Somily [Arabie saoudite]Source :
- Diagnostic Microbiology and Infectious Disease [ 0732-8893 ] ; 2017.
Descripteurs français
- KwdFr :
- Allemagne, Angleterre, Appareil respiratoire (microbiologie), Arabie saoudite, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Flambées de maladies, Humains, Infections de l'appareil respiratoire (microbiologie), Infections à coronavirus (microbiologie), RT-PCR (), Réaction de polymérisation en chaine en temps réel (), République de Corée.
- MESH :
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient.
- microbiologie : Appareil respiratoire, Infections de l'appareil respiratoire, Infections à coronavirus.
- Allemagne, Angleterre, Arabie saoudite, Flambées de maladies, Humains, RT-PCR, Réaction de polymérisation en chaine en temps réel, République de Corée.
English descriptors
- KwdEn :
- Coronavirus Infections (microbiology), Disease Outbreaks, England, Germany, Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Real-Time Polymerase Chain Reaction (methods), Republic of Korea, Respiratory System (microbiology), Respiratory Tract Infections (microbiology), Reverse Transcriptase Polymerase Chain Reaction (methods), Saudi Arabia.
- MESH :
- genetics : Middle East Respiratory Syndrome Coronavirus.
- methods : Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
- microbiology : Coronavirus Infections, Respiratory System, Respiratory Tract Infections.
- Disease Outbreaks, England, Germany, Humans, Republic of Korea, Saudi Arabia.
Abstract
Since discovery of Middle East respiratory syndrome coronavirus (MERS-CoV), a novel betacoronavirus first isolated and characterized in 2012, MERS-CoV real-time reverse transcriptase polymerase chain reaction (rRT-PCR) assays represent one of the most rapidly expanding commercial tests. However, in the absence of extensive evaluations of these assays on positive clinical material of different sources, evaluating their diagnostic effectiveness remains challenging. We describe the diagnostic performance evaluation of 3 common commercial MERS-CoV rRT-PCR assays on a large panel (n = 234) of upper respiratory tract specimens collected during an outbreak episode in Saudi Arabia. Assays were compared to the RealStar® MERS-CoV RT-PCR (Alton Diagnostics, Hamburg, Germany) assay as the gold standard. Results showed i) the TIB MolBiol® LightMix
Url:
DOI: 10.1016/j.diagmicrobio.2017.01.003
PubMed: 28104327
PubMed Central: 7132760
Affiliations:
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Le document en format XML
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Mohamed</name><affiliation wicri:level="1"><nlm:aff id="af0005">Pathology/Microbiology Division, College of Medicine, King Saud University, Riyadh, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Pathology/Microbiology Division, College of Medicine, King Saud University, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Alhetheel, Abdulkarim F" sort="Alhetheel, Abdulkarim F" uniqKey="Alhetheel A" first="Abdulkarim F." last="Alhetheel">Abdulkarim F. 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Alzamil</name><affiliation wicri:level="1"><nlm:aff id="af0020">Department of Pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Pediatrics, College of Medicine, King Saud University, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author><author><name sortKey="Somily, Ali M" sort="Somily, Ali M" uniqKey="Somily A" first="Ali M." last="Somily">Ali M. Somily</name><affiliation wicri:level="1"><nlm:aff id="af0005">Pathology/Microbiology Division, College of Medicine, King Saud University, Riyadh, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Pathology/Microbiology Division, College of Medicine, King Saud University, Riyadh</wicri:regionArea><wicri:noRegion>Riyadh</wicri:noRegion></affiliation></author></analytic><series><title level="j">Diagnostic Microbiology and Infectious Disease</title><idno type="ISSN">0732-8893</idno><idno type="eISSN">1879-0070</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus Infections (microbiology)</term><term>Disease Outbreaks</term><term>England</term><term>Germany</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Republic of Korea</term><term>Respiratory System (microbiology)</term><term>Respiratory Tract Infections (microbiology)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Saudi Arabia</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Allemagne</term><term>Angleterre</term><term>Appareil respiratoire (microbiologie)</term><term>Arabie saoudite</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Flambées de maladies</term><term>Humains</term><term>Infections de l'appareil respiratoire (microbiologie)</term><term>Infections à coronavirus (microbiologie)</term><term>RT-PCR ()</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>République de Corée</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Appareil respiratoire</term><term>Infections de l'appareil respiratoire</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Coronavirus Infections</term><term>Respiratory System</term><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Outbreaks</term><term>England</term><term>Germany</term><term>Humans</term><term>Republic of Korea</term><term>Saudi Arabia</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Allemagne</term><term>Angleterre</term><term>Arabie saoudite</term><term>Flambées de maladies</term><term>Humains</term><term>RT-PCR</term><term>Réaction de polymérisation en chaine en temps réel</term><term>République de Corée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Since discovery of Middle East respiratory syndrome coronavirus (MERS-CoV), a novel betacoronavirus first isolated and characterized in 2012, MERS-CoV real-time reverse transcriptase polymerase chain reaction (rRT-PCR) assays represent one of the most rapidly expanding commercial tests. However, in the absence of extensive evaluations of these assays on positive clinical material of different sources, evaluating their diagnostic effectiveness remains challenging. We describe the diagnostic performance evaluation of 3 common commercial MERS-CoV rRT-PCR assays on a large panel (n = 234) of upper respiratory tract specimens collected during an outbreak episode in Saudi Arabia. Assays were compared to the RealStar® MERS-CoV RT-PCR (Alton Diagnostics, Hamburg, Germany) assay as the gold standard. Results showed i) the TIB MolBiol® LightMix <italic>UpE</italic> and <italic>Orf1a</italic> assays (TIB MolBiol, Berlin, Germany) to be the most sensitive, followed by ii) the Anyplex™ Seegene MERS-CoV assay (Seegene, Seoul, Korea), and finally iii) the PrimerDesign™ Genesig® HCoV_2012 assay (PrimerDesign, England, United Kingdom). We also evaluate a modified protocol for the PrimerDesign™ Genesig® HCoV_2012 assay.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Eckerle, I" uniqKey="Eckerle I">I. Eckerle</name></author><author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T. Bleicker</name></author><author><name sortKey="Zaki, A" uniqKey="Zaki A">A. Zaki</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O. Landt</name></author><author><name sortKey="Eschbach Bludau, M" uniqKey="Eschbach Bludau M">M. Eschbach-Bludau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Müller</name></author><author><name sortKey="Costabel, U" uniqKey="Costabel U">U. 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Pas</name></author><author><name sortKey="Patel, P" uniqKey="Patel P">P. Patel</name></author><author><name sortKey="Reusken, C" uniqKey="Reusken C">C. Reusken</name></author><author><name sortKey="Domingo, C" uniqKey="Domingo C">C. Domingo</name></author><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Somily, A" uniqKey="Somily A">A. Somily</name></author><author><name sortKey="Barry, M" uniqKey="Barry M">M. Barry</name></author><author><name sortKey="Al Subaie, S" uniqKey="Al Subaie S">S. Al Subaie</name></author><author><name sortKey="Binsaeed, A" uniqKey="Binsaeed A">A. BinSaeed</name></author><author><name sortKey="Alzamil, F" uniqKey="Alzamil F">F. Alzamil</name></author><author><name sortKey="Zaher, W" uniqKey="Zaher W">W. 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Erdman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Arabie saoudite</li></country></list><tree><country name="Arabie saoudite"><noRegion><name sortKey="Mohamed, Deqa H" sort="Mohamed, Deqa H" uniqKey="Mohamed D" first="Deqa H." last="Mohamed">Deqa H. Mohamed</name></noRegion><name sortKey="Aldosari, Kamel" sort="Aldosari, Kamel" uniqKey="Aldosari K" first="Kamel" last="Aldosari">Kamel Aldosari</name><name sortKey="Alhetheel, Abdulkarim F" sort="Alhetheel, Abdulkarim F" uniqKey="Alhetheel A" first="Abdulkarim F." last="Alhetheel">Abdulkarim F. Alhetheel</name><name sortKey="Alzamil, Fahad A" sort="Alzamil, Fahad A" uniqKey="Alzamil F" first="Fahad A." last="Alzamil">Fahad A. Alzamil</name><name sortKey="Mohamud, Hanat S" sort="Mohamud, Hanat S" uniqKey="Mohamud H" first="Hanat S." last="Mohamud">Hanat S. Mohamud</name><name sortKey="Somily, Ali M" sort="Somily, Ali M" uniqKey="Somily A" first="Ali M." last="Somily">Ali M. Somily</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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