Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR
Identifieur interne : 000B92 ( Main/Exploration ); précédent : 000B91; suivant : 000B93Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR
Auteurs : Ji Yeong Noh [Corée du Sud] ; Sun-Woo Yoon [Corée du Sud] ; Doo-Jin Kim [Corée du Sud] ; Moo-Seung Lee [Corée du Sud] ; Ji-Hyung Kim [Corée du Sud] ; Woonsung Na [Corée du Sud] ; Daesub Song [Corée du Sud] ; Dae Gwin Jeong [Corée du Sud] ; Hye Kwon Kim [Corée du Sud]Source :
- Archives of Virology [ 0304-8608 ] ; 2017.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Alignement de séquences, Animaux, Chiroptera (virologie), Coronaviridae (génétique), Coronaviridae (isolement et purification), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Diagnostic différentiel, Expectoration (virologie), Fèces (virologie), Humains, Infections à coronavirus (virologie), Limite de détection, RT-PCR (), Réaction de polymérisation en chaine en temps réel (), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- génétique : ARN viral, Coronaviridae, Coronavirus du syndrome respiratoire du Moyen-Orient, Virus du SRAS.
- isolement et purification : Coronaviridae, Coronavirus du syndrome respiratoire du Moyen-Orient, Virus du SRAS.
- virologie : Chiroptera, Expectoration, Fèces, Infections à coronavirus, Syndrome respiratoire aigu sévère.
- Alignement de séquences, Animaux, Diagnostic différentiel, Humains, Limite de détection, RT-PCR, Réaction de polymérisation en chaine en temps réel.
English descriptors
- KwdEn :
- Animals, Chiroptera (virology), Coronaviridae (genetics), Coronaviridae (isolation & purification), Coronavirus Infections (virology), Diagnosis, Differential, Feces (virology), Humans, Limit of Detection, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), RNA, Viral (genetics), Real-Time Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (genetics), SARS Virus (isolation & purification), Sequence Alignment, Severe Acute Respiratory Syndrome (virology), Sputum (virology).
- MESH :
- chemical , genetics : RNA, Viral.
- genetics : Coronaviridae, Middle East Respiratory Syndrome Coronavirus, SARS Virus.
- isolation & purification : Coronaviridae, Middle East Respiratory Syndrome Coronavirus, SARS Virus.
- methods : Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Chiroptera, Coronavirus Infections, Feces, Severe Acute Respiratory Syndrome, Sputum.
- Animals, Diagnosis, Differential, Humans, Limit of Detection, Sequence Alignment.
Abstract
Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50–100 copies/mL and 5–100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats.
Url:
DOI: 10.1007/s00705-017-3281-9
PubMed: 28220326
PubMed Central: 7086956
Affiliations:
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id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1791 8264</institution-id><institution-id institution-id-type="GRID">grid.412786.e</institution-id><institution>Bio-Analytical Science Division,</institution><institution>Korea University of Science and Technology (UST),</institution></institution-wrap>Daejeon, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Daejeon</wicri:regionArea></affiliation></author><author><name sortKey="Kim, Ji Hyung" sort="Kim, Ji Hyung" uniqKey="Kim J" first="Ji-Hyung" last="Kim">Ji-Hyung Kim</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0636 3099</institution-id><institution-id institution-id-type="GRID">grid.249967.7</institution-id><institution>Infectious Disease Research Center,</institution><institution>Korea Research Institute of Bioscience and Biotechnology,</institution></institution-wrap>Daejeon, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Daejeon</wicri:regionArea></affiliation></author><author><name sortKey="Na, Woonsung" sort="Na, Woonsung" uniqKey="Na W" first="Woonsung" last="Na">Woonsung Na</name><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0840 2678</institution-id><institution-id institution-id-type="GRID">grid.222754.4</institution-id><institution>Department of Pharmacy, College of Pharmacy,</institution><institution>Korea University,</institution></institution-wrap>Sejong, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Sejong</wicri:regionArea></affiliation></author><author><name sortKey="Song, Daesub" sort="Song, Daesub" uniqKey="Song D" first="Daesub" last="Song">Daesub Song</name><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0840 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purification)</term><term>RNA, Viral (genetics)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sequence Alignment</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Sputum (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Alignement de séquences</term><term>Animaux</term><term>Chiroptera (virologie)</term><term>Coronaviridae (génétique)</term><term>Coronaviridae (isolement et purification)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Diagnostic différentiel</term><term>Expectoration (virologie)</term><term>Fèces (virologie)</term><term>Humains</term><term>Infections à coronavirus (virologie)</term><term>Limite de détection</term><term>RT-PCR ()</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronaviridae</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Coronaviridae</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronaviridae</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronaviridae</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term><term>Expectoration</term><term>Fèces</term><term>Infections à coronavirus</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term><term>Coronavirus Infections</term><term>Feces</term><term>Severe Acute Respiratory Syndrome</term><term>Sputum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Diagnosis, Differential</term><term>Humans</term><term>Limit of Detection</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Animaux</term><term>Diagnostic différentiel</term><term>Humains</term><term>Limite de détection</term><term>RT-PCR</term><term>Réaction de polymérisation en chaine en temps réel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50–100 copies/mL and 5–100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Su, S" uniqKey="Su S">S Su</name></author><author><name sortKey="Wong, G" uniqKey="Wong G">G Wong</name></author><author><name sortKey="Shi, W" uniqKey="Shi W">W Shi</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="Lai, Ack" uniqKey="Lai A">ACK Lai</name></author><author><name sortKey="Zhou, J" uniqKey="Zhou J">J Zhou</name></author><author><name sortKey="Liu, W" uniqKey="Liu W">W Liu</name></author><author><name sortKey="Bi, Y" uniqKey="Bi Y">Y Bi</name></author><author><name sortKey="Gao, Gf" uniqKey="Gao G">GF Gao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drexler, Jf" uniqKey="Drexler J">JF 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uniqKey="Noh J" first="Ji Yeong" last="Noh">Ji Yeong Noh</name></noRegion><name sortKey="Jeong, Dae Gwin" sort="Jeong, Dae Gwin" uniqKey="Jeong D" first="Dae Gwin" last="Jeong">Dae Gwin Jeong</name><name sortKey="Jeong, Dae Gwin" sort="Jeong, Dae Gwin" uniqKey="Jeong D" first="Dae Gwin" last="Jeong">Dae Gwin Jeong</name><name sortKey="Kim, Doo Jin" sort="Kim, Doo Jin" uniqKey="Kim D" first="Doo-Jin" last="Kim">Doo-Jin Kim</name><name sortKey="Kim, Hye Kwon" sort="Kim, Hye Kwon" uniqKey="Kim H" first="Hye Kwon" last="Kim">Hye Kwon Kim</name><name sortKey="Kim, Ji Hyung" sort="Kim, Ji Hyung" uniqKey="Kim J" first="Ji-Hyung" last="Kim">Ji-Hyung Kim</name><name sortKey="Lee, Moo Seung" sort="Lee, Moo Seung" uniqKey="Lee M" first="Moo-Seung" last="Lee">Moo-Seung Lee</name><name sortKey="Lee, Moo Seung" sort="Lee, Moo Seung" uniqKey="Lee M" first="Moo-Seung" last="Lee">Moo-Seung Lee</name><name sortKey="Na, Woonsung" sort="Na, Woonsung" uniqKey="Na W" first="Woonsung" last="Na">Woonsung Na</name><name sortKey="Noh, Ji Yeong" sort="Noh, Ji Yeong" uniqKey="Noh J" first="Ji Yeong" last="Noh">Ji Yeong Noh</name><name sortKey="Song, Daesub" sort="Song, Daesub" uniqKey="Song D" first="Daesub" last="Song">Daesub Song</name><name sortKey="Yoon, Sun Woo" sort="Yoon, Sun Woo" uniqKey="Yoon S" first="Sun-Woo" last="Yoon">Sun-Woo Yoon</name><name sortKey="Yoon, Sun Woo" sort="Yoon, Sun Woo" uniqKey="Yoon S" first="Sun-Woo" last="Yoon">Sun-Woo Yoon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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