Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus☆☆☆
Identifieur interne : 000932 ( Main/Exploration ); précédent : 000931; suivant : 000933Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus☆☆☆
Auteurs : Justin Hardick [États-Unis] ; David Metzgar [États-Unis] ; Lisa Risen [États-Unis] ; Christopher Myers [États-Unis] ; Melinda Balansay [États-Unis] ; Trent Malcom [États-Unis] ; Richard Rothman [États-Unis] ; Charlotte Gaydos [États-Unis]Source :
- Diagnostic Microbiology and Infectious Disease [ 0732-8893 ] ; 2018.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Femelle, Grippe humaine (diagnostic), Humains, Infections à coronavirus (diagnostic), Infections à virus respiratoire syncytial (diagnostic), Jeune adulte, Mâle, RT-PCR, Sujet âgé, Sujet âgé de 80 ans ou plus, Techniques de diagnostic moléculaire (), Techniques de diagnostic moléculaire (instrumentation), Virus de la grippe A (génétique), Virus de la grippe A (isolement et purification), Virus influenza B (génétique), Virus influenza B (isolement et purification), Virus respiratoire syncytial humain (génétique), Virus respiratoire syncytial humain (isolement et purification).
- MESH :
- diagnostic : Grippe humaine, Infections à coronavirus, Infections à virus respiratoire syncytial.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient, Virus de la grippe A, Virus influenza B, Virus respiratoire syncytial humain.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient, Virus de la grippe A, Virus influenza B, Virus respiratoire syncytial humain.
- instrumentation : Adolescent, Adulte, Adulte d'âge moyen, Femelle, Humains, Jeune adulte, Mâle, RT-PCR, Sujet âgé, Sujet âgé de 80 ans ou plus, Techniques de diagnostic moléculaire.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Coronavirus Infections (diagnosis), Female, Humans, Influenza A virus (genetics), Influenza A virus (isolation & purification), Influenza B virus (genetics), Influenza B virus (isolation & purification), Influenza, Human (diagnosis), Male, Middle Aged, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (instrumentation), Molecular Diagnostic Techniques (methods), Respiratory Syncytial Virus Infections (diagnosis), Respiratory Syncytial Virus, Human (genetics), Respiratory Syncytial Virus, Human (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction, Young Adult.
- MESH :
- diagnosis : Coronavirus Infections, Influenza, Human, Respiratory Syncytial Virus Infections.
- genetics : Influenza A virus, Influenza B virus, Middle East Respiratory Syndrome Coronavirus, Respiratory Syncytial Virus, Human.
- instrumentation : Molecular Diagnostic Techniques.
- isolation & purification : Influenza A virus, Influenza B virus, Middle East Respiratory Syndrome Coronavirus, Respiratory Syncytial Virus, Human.
- methods : Molecular Diagnostic Techniques.
- Adolescent, Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction, Young Adult.
Abstract
Clinical samples were evaluated with the Mobile Analysis Platform (MAP) to determine platform performance for detecting respiratory viruses in samples previously characterized using clinical reverse transcriptase polymerase chain reaction assays. The percent agreement between MAP and clinical results was 97% for influenza A (73/75), 100% (21/21) for influenza B, 100% (6/6) for respiratory syncytial virus (RSV), and 80% (4/5) for negative specimens. The approximate limit of detection of the MAP was 30 copies/assay for RSV and 1500 copies/assay for Middle East respiratory syndrome coronavirus.
Url:
DOI: 10.1016/j.diagmicrobio.2018.02.011
PubMed: 29550057
PubMed Central: 6013072
Affiliations:
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Le document en format XML
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type="main">Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus<sup><xref ref-type="fn" rid="d32e608">☆</xref></sup><sup><xref ref-type="fn" rid="d32e615">☆☆</xref></sup></title><author><name sortKey="Hardick, Justin" sort="Hardick, Justin" uniqKey="Hardick J" first="Justin" last="Hardick">Justin Hardick</name><affiliation wicri:level="2"><nlm:aff id="af0005">Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Metzgar, David" sort="Metzgar, David" uniqKey="Metzgar D" first="David" last="Metzgar">David Metzgar</name><affiliation wicri:level="2"><nlm:aff id="af0010">Ibis Biosciences, Carlsbad, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Ibis Biosciences, Carlsbad, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Risen, Lisa" sort="Risen, Lisa" uniqKey="Risen L" first="Lisa" last="Risen">Lisa Risen</name><affiliation wicri:level="2"><nlm:aff id="af0010">Ibis Biosciences, Carlsbad, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Ibis Biosciences, Carlsbad, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Myers, Christopher" sort="Myers, Christopher" uniqKey="Myers C" first="Christopher" last="Myers">Christopher Myers</name><affiliation wicri:level="2"><nlm:aff id="af0015">Naval Health Research Center, San Diego, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Naval Health Research Center, San Diego, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Balansay, Melinda" sort="Balansay, Melinda" uniqKey="Balansay M" first="Melinda" last="Balansay">Melinda Balansay</name><affiliation wicri:level="2"><nlm:aff id="af0015">Naval Health Research Center, San Diego, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Naval Health Research Center, San Diego, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Malcom, Trent" sort="Malcom, Trent" uniqKey="Malcom T" first="Trent" last="Malcom">Trent Malcom</name><affiliation wicri:level="2"><nlm:aff id="af0020">Johns Hopkins University Department of Emergency Medicine, Baltimore, MD, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Johns Hopkins University Department of Emergency Medicine, Baltimore, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Rothman, Richard" sort="Rothman, Richard" uniqKey="Rothman R" first="Richard" last="Rothman">Richard Rothman</name><affiliation wicri:level="2"><nlm:aff id="af0020">Johns Hopkins University Department of Emergency Medicine, Baltimore, MD, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Johns Hopkins University Department of Emergency Medicine, Baltimore, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Gaydos, Charlotte" sort="Gaydos, Charlotte" uniqKey="Gaydos C" first="Charlotte" last="Gaydos">Charlotte Gaydos</name><affiliation wicri:level="2"><nlm:aff id="af0005">Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><series><title level="j">Diagnostic Microbiology and Infectious Disease</title><idno type="ISSN">0732-8893</idno><idno type="eISSN">1879-0070</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Coronavirus Infections (diagnosis)</term><term>Female</term><term>Humans</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (isolation & purification)</term><term>Influenza B virus (genetics)</term><term>Influenza B virus (isolation & purification)</term><term>Influenza, Human (diagnosis)</term><term>Male</term><term>Middle Aged</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Molecular Diagnostic Techniques (instrumentation)</term><term>Molecular Diagnostic Techniques (methods)</term><term>Respiratory Syncytial Virus Infections (diagnosis)</term><term>Respiratory Syncytial Virus, Human (genetics)</term><term>Respiratory Syncytial Virus, Human (isolation & purification)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Femelle</term><term>Grippe humaine (diagnostic)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à virus respiratoire syncytial (diagnostic)</term><term>Jeune adulte</term><term>Mâle</term><term>RT-PCR</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Techniques de diagnostic moléculaire ()</term><term>Techniques de diagnostic moléculaire (instrumentation)</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (isolement et purification)</term><term>Virus influenza B (génétique)</term><term>Virus influenza B (isolement et purification)</term><term>Virus respiratoire syncytial humain (génétique)</term><term>Virus respiratoire syncytial humain (isolement et purification)</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Influenza, Human</term><term>Respiratory Syncytial Virus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term><term>Infections à coronavirus</term><term>Infections à virus respiratoire syncytial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term><term>Influenza B virus</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Respiratory Syncytial Virus, Human</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Virus de la grippe A</term><term>Virus influenza B</term><term>Virus respiratoire syncytial humain</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Molecular Diagnostic Techniques</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term><term>Influenza B virus</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>Respiratory Syncytial Virus, Human</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Virus de la grippe A</term><term>Virus influenza B</term><term>Virus respiratoire syncytial humain</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Young Adult</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>RT-PCR</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term><term>Techniques de diagnostic moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Clinical samples were evaluated with the Mobile Analysis Platform (MAP) to determine platform performance for detecting respiratory viruses in samples previously characterized using clinical reverse transcriptase polymerase chain reaction assays. The percent agreement between MAP and clinical results was 97% for influenza A (73/75), 100% (21/21) for influenza B, 100% (6/6) for respiratory syncytial virus (RSV), and 80% (4/5) for negative specimens. The approximate limit of detection of the MAP was 30 copies/assay for RSV and 1500 copies/assay for Middle East respiratory syndrome coronavirus.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Berry, M" uniqKey="Berry M">M. Berry</name></author><author><name sortKey="Gamieldien, J" uniqKey="Gamieldien J">J. Gamieldien</name></author><author><name sortKey="Fielding, B C" uniqKey="Fielding B">B.C. Fielding</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bhadra, S" uniqKey="Bhadra S">S. Bhadra</name></author><author><name sortKey="Jiang, Y S" uniqKey="Jiang Y">Y.S. Jiang</name></author><author><name sortKey="Kumar, M R" uniqKey="Kumar M">M.R. 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Balasubramani</name></author><author><name sortKey="Moehling, K K" uniqKey="Moehling K">K.K. Moehling</name></author><author><name sortKey="Bullotta, A" uniqKey="Bullotta A">A. Bullotta</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zumla, A" uniqKey="Zumla A">A. Zumla</name></author><author><name sortKey="Al Tawfiq, J A" uniqKey="Al Tawfiq J">J.A. Al-Tawfiq</name></author><author><name sortKey="Enne, V I" uniqKey="Enne V">V.I. Enne</name></author><author><name sortKey="Kidd, M" uniqKey="Kidd M">M. Kidd</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Breuer, J" uniqKey="Breuer J">J. Breuer</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Hardick, Justin" sort="Hardick, Justin" uniqKey="Hardick J" first="Justin" last="Hardick">Justin Hardick</name></region><name sortKey="Balansay, Melinda" sort="Balansay, Melinda" uniqKey="Balansay M" first="Melinda" last="Balansay">Melinda Balansay</name><name sortKey="Gaydos, Charlotte" sort="Gaydos, Charlotte" uniqKey="Gaydos C" first="Charlotte" last="Gaydos">Charlotte Gaydos</name><name sortKey="Malcom, Trent" sort="Malcom, Trent" uniqKey="Malcom T" first="Trent" last="Malcom">Trent Malcom</name><name sortKey="Metzgar, David" sort="Metzgar, David" uniqKey="Metzgar D" first="David" last="Metzgar">David Metzgar</name><name sortKey="Myers, Christopher" sort="Myers, Christopher" uniqKey="Myers C" first="Christopher" last="Myers">Christopher Myers</name><name sortKey="Risen, Lisa" sort="Risen, Lisa" uniqKey="Risen L" first="Lisa" last="Risen">Lisa Risen</name><name sortKey="Rothman, Richard" sort="Rothman, Richard" uniqKey="Rothman R" first="Richard" last="Rothman">Richard Rothman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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