The prevalence of Middle East respiratory Syndrome coronavirus (MERS-CoV) infection in livestock and temporal relation to locations and seasons
Identifieur interne : 000776 ( Main/Exploration ); précédent : 000775; suivant : 000777The prevalence of Middle East respiratory Syndrome coronavirus (MERS-CoV) infection in livestock and temporal relation to locations and seasons
Auteurs : Samy Kasem [Arabie saoudite, Égypte] ; Ibrahim Qasim [Arabie saoudite] ; Ali Al-Doweriej [Arabie saoudite] ; Osman Hashim [Arabie saoudite] ; Ali Alkarar [Arabie saoudite] ; Ali Abu-Obeida [Arabie saoudite] ; Mohamed Saleh [Arabie saoudite] ; Ali Al-Hofufi [Arabie saoudite] ; Hussein Al-Ghadier [Arabie saoudite] ; Raed Hussien [Arabie saoudite] ; Ali Al-Sahaf [Arabie saoudite] ; Faisal Bayoumi [Arabie saoudite] ; Asmaa Magouz [Égypte]Source :
- Journal of Infection and Public Health [ 1876-0341 ] ; 2018.
Descripteurs français
- KwdFr :
- Animaux, Arabie saoudite (épidémiologie), Bétail (virologie), Chameaux (virologie), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Dosage immunologique (), Fosse nasale (virologie), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Prévalence, RT-PCR (), Réaction de polymérisation en chaine en temps réel (), Saisons, Sensibilité et spécificité, Tests diagnostiques courants (), Topographie médicale.
- MESH :
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- médecine vétérinaire : Infections à coronavirus.
- virologie : Bétail, Chameaux, Fosse nasale, Infections à coronavirus.
- épidémiologie : Arabie saoudite, Infections à coronavirus.
- Animaux, Dosage immunologique, Prévalence, RT-PCR, Réaction de polymérisation en chaine en temps réel, Saisons, Sensibilité et spécificité, Tests diagnostiques courants, Topographie médicale.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Animals, Camelus (virology), Coronavirus Infections (epidemiology), Coronavirus Infections (veterinary), Coronavirus Infections (virology), Diagnostic Tests, Routine (methods), Immunoassay (methods), Livestock (virology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Nasal Cavity (virology), Prevalence, Real-Time Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (methods), Saudi Arabia (epidemiology), Seasons, Sensitivity and Specificity, Topography, Medical, Whole Genome Sequencing.
- MESH :
- geographic , epidemiology : Saudi Arabia.
- epidemiology : Coronavirus Infections.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Diagnostic Tests, Routine, Immunoassay, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
- veterinary : Coronavirus Infections.
- virology : Camelus, Coronavirus Infections, Livestock, Nasal Cavity.
- Animals, Prevalence, Seasons, Sensitivity and Specificity, Topography, Medical, Whole Genome Sequencing.
Abstract
The Middle East respiratory syndrome (MERS) has been reported for the first time infecting a human being since 2012. The WHO was notified of 27 countries have reported cases of MERS, the majority of these cases occur in the Arabian Peninsula, particularly in Saudi Arabia. Dromedary camels are likely to be the main source of Middle East respiratory syndrome virus (MERS-CoV) infection in humans.
MERS-CoV infection rates among camels in livestock markets and slaughterhouses were investigated in Saudi Arabia. A total of 698 nasal swabs were collected and examined with Rapid assay and rtRT-PCR. Ten MERS-CoV positive samples were subjected to full genomic sequencing. In addition, the sensitivity and specificity of the Rapid immunochromatographic assay (BioNote, South Korea) was evaluated as a diagnostic tool for MERS-CoV compared to rtRT-PCR.
The results showed a high percentage of dromedaries (56.4%) had evidence for nasal MERS-CoV infection. Phylogenetic analysis of the ten MERS-CoV isolates showed that the sequences were closely related to the other MERS-CoV strains recovered from camels and human cases. Moreover, the results showed that 195 samples were positive for MERS-CoV by rapid assay compared to 394 positive samples of rtRT-PCR, which showed low rapid assay sensitivity (49.49%) while, the specificity were found to be 100%.
These findings indicate that these sites are a highly-hazardous to zoonotic diseases.
Url:
DOI: 10.1016/j.jiph.2018.01.004
PubMed: 29396257
PubMed Central: 7102711
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000719
- to stream Pmc, to step Curation: 000719
- to stream Pmc, to step Checkpoint: 000449
- to stream PubMed, to step Corpus: 000A04
- to stream PubMed, to step Curation: 000A04
- to stream PubMed, to step Checkpoint: 002991
- to stream Ncbi, to step Merge: 001D27
- to stream Ncbi, to step Curation: 001D27
- to stream Ncbi, to step Checkpoint: 001D27
- to stream Main, to step Merge: 000779
- to stream Main, to step Curation: 000776
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The prevalence of Middle East respiratory Syndrome coronavirus (MERS-CoV) infection in livestock and temporal relation to locations and seasons</title><author><name sortKey="Kasem, Samy" sort="Kasem, Samy" uniqKey="Kasem S" first="Samy" last="Kasem">Samy Kasem</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh,El-Geish Street, 33516, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh,El-Geish Street, 33516</wicri:regionArea><wicri:noRegion>33516</wicri:noRegion></affiliation></author><author><name sortKey="Qasim, Ibrahim" sort="Qasim, Ibrahim" uniqKey="Qasim I" first="Ibrahim" last="Qasim">Ibrahim Qasim</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Doweriej, Ali" sort="Al Doweriej, Ali" uniqKey="Al Doweriej A" first="Ali" last="Al-Doweriej">Ali Al-Doweriej</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Hashim, Osman" sort="Hashim, Osman" uniqKey="Hashim O" first="Osman" last="Hashim">Osman Hashim</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Alkarar, Ali" sort="Alkarar, Ali" uniqKey="Alkarar A" first="Ali" last="Alkarar">Ali Alkarar</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Abu Obeida, Ali" sort="Abu Obeida, Ali" uniqKey="Abu Obeida A" first="Ali" last="Abu-Obeida">Ali Abu-Obeida</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Saleh, Mohamed" sort="Saleh, Mohamed" uniqKey="Saleh M" first="Mohamed" last="Saleh">Mohamed Saleh</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Hofufi, Ali" sort="Al Hofufi, Ali" uniqKey="Al Hofufi A" first="Ali" last="Al-Hofufi">Ali Al-Hofufi</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Ghadier, Hussein" sort="Al Ghadier, Hussein" uniqKey="Al Ghadier H" first="Hussein" last="Al-Ghadier">Hussein Al-Ghadier</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Hussien, Raed" sort="Hussien, Raed" uniqKey="Hussien R" first="Raed" last="Hussien">Raed Hussien</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Sahaf, Ali" sort="Al Sahaf, Ali" uniqKey="Al Sahaf A" first="Ali" last="Al-Sahaf">Ali Al-Sahaf</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Bayoumi, Faisal" sort="Bayoumi, Faisal" uniqKey="Bayoumi F" first="Faisal" last="Bayoumi">Faisal Bayoumi</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Magouz, Asmaa" sort="Magouz, Asmaa" uniqKey="Magouz A" first="Asmaa" last="Magouz">Asmaa Magouz</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh,El-Geish Street, 33516, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh,El-Geish Street, 33516</wicri:regionArea><wicri:noRegion>33516</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">29396257</idno><idno type="pmc">7102711</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102711</idno><idno type="RBID">PMC:7102711</idno><idno type="doi">10.1016/j.jiph.2018.01.004</idno><date when="2018">2018</date><idno type="wicri:Area/Pmc/Corpus">000719</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000719</idno><idno type="wicri:Area/Pmc/Curation">000719</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000719</idno><idno type="wicri:Area/Pmc/Checkpoint">000449</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000449</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:29396257</idno><idno type="wicri:Area/PubMed/Corpus">000A04</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000A04</idno><idno type="wicri:Area/PubMed/Curation">000A04</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000A04</idno><idno type="wicri:Area/PubMed/Checkpoint">002991</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002991</idno><idno type="wicri:Area/Ncbi/Merge">001D27</idno><idno type="wicri:Area/Ncbi/Curation">001D27</idno><idno type="wicri:Area/Ncbi/Checkpoint">001D27</idno><idno type="wicri:doubleKey">1876-0341:2018:Kasem S:the:prevalence:of</idno><idno type="wicri:Area/Main/Merge">000779</idno><idno type="wicri:Area/Main/Curation">000776</idno><idno type="wicri:Area/Main/Exploration">000776</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The prevalence of Middle East respiratory Syndrome coronavirus (MERS-CoV) infection in livestock and temporal relation to locations and seasons</title><author><name sortKey="Kasem, Samy" sort="Kasem, Samy" uniqKey="Kasem S" first="Samy" last="Kasem">Samy Kasem</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh,El-Geish Street, 33516, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh,El-Geish Street, 33516</wicri:regionArea><wicri:noRegion>33516</wicri:noRegion></affiliation></author><author><name sortKey="Qasim, Ibrahim" sort="Qasim, Ibrahim" uniqKey="Qasim I" first="Ibrahim" last="Qasim">Ibrahim Qasim</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Doweriej, Ali" sort="Al Doweriej, Ali" uniqKey="Al Doweriej A" first="Ali" last="Al-Doweriej">Ali Al-Doweriej</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Hashim, Osman" sort="Hashim, Osman" uniqKey="Hashim O" first="Osman" last="Hashim">Osman Hashim</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Alkarar, Ali" sort="Alkarar, Ali" uniqKey="Alkarar A" first="Ali" last="Alkarar">Ali Alkarar</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Abu Obeida, Ali" sort="Abu Obeida, Ali" uniqKey="Abu Obeida A" first="Ali" last="Abu-Obeida">Ali Abu-Obeida</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Saleh, Mohamed" sort="Saleh, Mohamed" uniqKey="Saleh M" first="Mohamed" last="Saleh">Mohamed Saleh</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Hofufi, Ali" sort="Al Hofufi, Ali" uniqKey="Al Hofufi A" first="Ali" last="Al-Hofufi">Ali Al-Hofufi</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Ghadier, Hussein" sort="Al Ghadier, Hussein" uniqKey="Al Ghadier H" first="Hussein" last="Al-Ghadier">Hussein Al-Ghadier</name><affiliation wicri:level="1"><nlm:aff id="aff0020">Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Laboratory, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Hussien, Raed" sort="Hussien, Raed" uniqKey="Hussien R" first="Raed" last="Hussien">Raed Hussien</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Al Sahaf, Ali" sort="Al Sahaf, Ali" uniqKey="Al Sahaf A" first="Ali" last="Al-Sahaf">Ali Al-Sahaf</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Services, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Bayoumi, Faisal" sort="Bayoumi, Faisal" uniqKey="Bayoumi F" first="Faisal" last="Bayoumi">Faisal Bayoumi</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195, Saudi Arabia</nlm:aff><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Department of Veterinary Health and Monitoring, Ministry of Environment, Water and Agriculture,65 King Abdulaziz Road, Riyadh, 11195</wicri:regionArea><wicri:noRegion>11195</wicri:noRegion></affiliation></author><author><name sortKey="Magouz, Asmaa" sort="Magouz, Asmaa" uniqKey="Magouz A" first="Asmaa" last="Magouz">Asmaa Magouz</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh,El-Geish Street, 33516, Egypt</nlm:aff><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh,El-Geish Street, 33516</wicri:regionArea><wicri:noRegion>33516</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Infection and Public Health</title><idno type="ISSN">1876-0341</idno><idno type="eISSN">1876-035X</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Camelus (virology)</term><term>Coronavirus Infections (epidemiology)</term><term>Coronavirus Infections (veterinary)</term><term>Coronavirus Infections (virology)</term><term>Diagnostic Tests, Routine (methods)</term><term>Immunoassay (methods)</term><term>Livestock (virology)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Nasal Cavity (virology)</term><term>Prevalence</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Saudi Arabia (epidemiology)</term><term>Seasons</term><term>Sensitivity and Specificity</term><term>Topography, Medical</term><term>Whole Genome Sequencing</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Arabie saoudite (épidémiologie)</term><term>Bétail (virologie)</term><term>Chameaux (virologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Dosage immunologique ()</term><term>Fosse nasale (virologie)</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Infections à coronavirus (virologie)</term><term>Infections à coronavirus (épidémiologie)</term><term>Prévalence</term><term>RT-PCR ()</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Saisons</term><term>Sensibilité et spécificité</term><term>Tests diagnostiques courants ()</term><term>Topographie médicale</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Diagnostic Tests, Routine</term><term>Immunoassay</term><term>Real-Time Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Bétail</term><term>Chameaux</term><term>Fosse nasale</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Camelus</term><term>Coronavirus Infections</term><term>Livestock</term><term>Nasal Cavity</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Arabie saoudite</term><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Prevalence</term><term>Seasons</term><term>Sensitivity and Specificity</term><term>Topography, Medical</term><term>Whole Genome Sequencing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Dosage immunologique</term><term>Prévalence</term><term>RT-PCR</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Saisons</term><term>Sensibilité et spécificité</term><term>Tests diagnostiques courants</term><term>Topographie médicale</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The Middle East respiratory syndrome (MERS) has been reported for the first time infecting a human being since 2012. The WHO was notified of 27 countries have reported cases of MERS, the majority of these cases occur in the Arabian Peninsula, particularly in Saudi Arabia. Dromedary camels are likely to be the main source of Middle East respiratory syndrome virus (MERS-CoV) infection in humans.</p></sec><sec><title>Methods</title><p>MERS-CoV infection rates among camels in livestock markets and slaughterhouses were investigated in Saudi Arabia. A total of 698 nasal swabs were collected and examined with Rapid assay and rtRT-PCR. Ten MERS-CoV positive samples were subjected to full genomic sequencing. In addition, the sensitivity and specificity of the Rapid immunochromatographic assay (BioNote, South Korea) was evaluated as a diagnostic tool for MERS-CoV compared to rtRT-PCR.</p></sec><sec><title>Results</title><p>The results showed a high percentage of dromedaries (56.4%) had evidence for nasal MERS-CoV infection. Phylogenetic analysis of the ten MERS-CoV isolates showed that the sequences were closely related to the other MERS-CoV strains recovered from camels and human cases. Moreover, the results showed that 195 samples were positive for MERS-CoV by rapid assay compared to 394 positive samples of rtRT-PCR, which showed low rapid assay sensitivity (49.49%) while, the specificity were found to be 100%.</p></sec><sec><title>Conclusion</title><p>These findings indicate that these sites are a highly-hazardous to zoonotic diseases.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="World Health Organization" uniqKey="World Health Organization">World Health Organization</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bermingham, A" uniqKey="Bermingham A">A. Bermingham</name></author><author><name sortKey="Chand, M A" uniqKey="Chand M">M.A. Chand</name></author><author><name sortKey="Brown, C S" uniqKey="Brown C">C.S. Brown</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ecdc" uniqKey="Ecdc">ECDC</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Jores, J" uniqKey="Jores J">J. Jores</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author><author><name sortKey="Younan, M" uniqKey="Younan M">M. Younan</name></author><author><name sortKey="Liljander, A" uniqKey="Liljander A">A. Liljander</name></author><author><name sortKey="Said, M Y" uniqKey="Said M">M.Y. Said</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kasem, S" uniqKey="Kasem S">S. Kasem</name></author><author><name sortKey="Qasim, I" uniqKey="Qasim I">I. Qasim</name></author><author><name sortKey="Al Hufofi, A" uniqKey="Al Hufofi A">A. Al-Hufofi</name></author><author><name sortKey="Hashim, O" uniqKey="Hashim O">O. Hashim</name></author><author><name sortKey="Alkarar, A" uniqKey="Alkarar A">A. Alkarar</name></author><author><name sortKey="Abu Obeida, A" uniqKey="Abu Obeida A">A. Abu-Obeida</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ali, M A" uniqKey="Ali M">M.A. Ali</name></author><author><name sortKey="Shehata, M M" uniqKey="Shehata M">M.M. Shehata</name></author><author><name sortKey="Gomaa, M R" uniqKey="Gomaa M">M.R. Gomaa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saqib, M" uniqKey="Saqib M">M. Saqib</name></author><author><name sortKey="Sieberg, A" uniqKey="Sieberg A">A. Sieberg</name></author><author><name sortKey="Hussain, M" uniqKey="Hussain M">M. Hussain</name></author><author><name sortKey="Mansoor, M" uniqKey="Mansoor M">M. Mansoor</name></author><author><name sortKey="Zohaib, A" uniqKey="Zohaib A">A. Zohaib</name></author><author><name sortKey="Lattwein, E" uniqKey="Lattwein E">E. Lattwein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alagaili, A N" uniqKey="Alagaili A">A.N. Alagaili</name></author><author><name sortKey="Briese, T" uniqKey="Briese T">T. Briese</name></author><author><name sortKey="Mishra, N" uniqKey="Mishra N">N. Mishra</name></author><author><name sortKey="Kapoor, V" uniqKey="Kapoor V">V. Kapoor</name></author><author><name sortKey="Sameroff, S C" uniqKey="Sameroff S">S.C. Sameroff</name></author><author><name sortKey="Dewit, E" uniqKey="Dewit E">E. deWit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Azhar, E I" uniqKey="Azhar E">E.I. Azhar</name></author><author><name sortKey="El Kafrawy, S A" uniqKey="El Kafrawy S">S.A. El-Kafrawy</name></author><author><name sortKey="Farraj, S A" uniqKey="Farraj S">S.A. Farraj</name></author><author><name sortKey="Hassan, A M" uniqKey="Hassan A">A.M. Hassan</name></author><author><name sortKey="Al Saeed, M S" uniqKey="Al Saeed M">M.S. Al-Saeed</name></author><author><name sortKey="Hashem, A M M T" uniqKey="Hashem A">A.M.M.T. Hashem</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Briese, T" uniqKey="Briese T">T. Briese</name></author><author><name sortKey="Mishra, N" uniqKey="Mishra N">N. Mishra</name></author><author><name sortKey="Jain, K" uniqKey="Jain K">K. Jain</name></author><author><name sortKey="Zalmout, I S" uniqKey="Zalmout I">I.S. Zalmout</name></author><author><name sortKey="Jabado, O J" uniqKey="Jabado O">O.J. Jabado</name></author><author><name sortKey="Karesh, W B" uniqKey="Karesh W">W.B. Karesh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oie" uniqKey="Oie">OIE</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Eckerle, I" uniqKey="Eckerle I">I. Eckerle</name></author><author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T. Bleicker</name></author><author><name sortKey="Zaki, A" uniqKey="Zaki A">A. Zaki</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O. Landt</name></author><author><name sortKey="Eschbach Bludau, M" uniqKey="Eschbach Bludau M">M. Eschbach-Bludau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Costabel, U" uniqKey="Costabel U">U. Costabel</name></author><author><name sortKey="Timm, J" uniqKey="Timm J">J. Timm</name></author><author><name sortKey="Binger, T" uniqKey="Binger T">T. Binger</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Kumar, S" uniqKey="Kumar S">S. Kumar</name></author><author><name sortKey="Stecher, G" uniqKey="Stecher G">G. Stecher</name></author><author><name sortKey="Tamura, K" uniqKey="Tamura K">K. Tamura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Khalafalla, A I" uniqKey="Khalafalla A">A.I. Khalafalla</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X. Lu</name></author><author><name sortKey="Al Mubarak, A" uniqKey="Al Mubarak A">A. Al-Mubarak</name></author><author><name sortKey="Dalab, A S" uniqKey="Dalab A">A.S. Dalab</name></author><author><name sortKey="Al Busadah, K" uniqKey="Al Busadah K">K. Al-Busadah</name></author><author><name sortKey="Erdman, D D" uniqKey="Erdman D">D.D. Erdman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Farag, E A" uniqKey="Farag E">E.A. Farag</name></author><author><name sortKey="Reusken, C B" uniqKey="Reusken C">C.B. Reusken</name></author><author><name sortKey="Haagmans, B H" uniqKey="Haagmans B">B.H. Haagmans</name></author><author><name sortKey="Khaled Mohran, A K A" uniqKey="Khaled Mohran A">A.K.A. Khaled Mohran</name></author><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name></author><author><name sortKey="Pas, S D" uniqKey="Pas S">S.D. Pas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, D" uniqKey="Song D">D. Song</name></author><author><name sortKey="Ha, G" uniqKey="Ha G">G. Ha</name></author><author><name sortKey="Serhan, W" uniqKey="Serhan W">W. Serhan</name></author><author><name sortKey="Eltahir, Y" uniqKey="Eltahir Y">Y. Eltahir</name></author><author><name sortKey="Yusof, M" uniqKey="Yusof M">M. Yusof</name></author><author><name sortKey="Hashem, F" uniqKey="Hashem F">F. Hashem</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Arabie saoudite</li><li>Égypte</li></country></list><tree><country name="Arabie saoudite"><noRegion><name sortKey="Kasem, Samy" sort="Kasem, Samy" uniqKey="Kasem S" first="Samy" last="Kasem">Samy Kasem</name></noRegion><name sortKey="Abu Obeida, Ali" sort="Abu Obeida, Ali" uniqKey="Abu Obeida A" first="Ali" last="Abu-Obeida">Ali Abu-Obeida</name><name sortKey="Al Doweriej, Ali" sort="Al Doweriej, Ali" uniqKey="Al Doweriej A" first="Ali" last="Al-Doweriej">Ali Al-Doweriej</name><name sortKey="Al Ghadier, Hussein" sort="Al Ghadier, Hussein" uniqKey="Al Ghadier H" first="Hussein" last="Al-Ghadier">Hussein Al-Ghadier</name><name sortKey="Al Hofufi, Ali" sort="Al Hofufi, Ali" uniqKey="Al Hofufi A" first="Ali" last="Al-Hofufi">Ali Al-Hofufi</name><name sortKey="Al Sahaf, Ali" sort="Al Sahaf, Ali" uniqKey="Al Sahaf A" first="Ali" last="Al-Sahaf">Ali Al-Sahaf</name><name sortKey="Alkarar, Ali" sort="Alkarar, Ali" uniqKey="Alkarar A" first="Ali" last="Alkarar">Ali Alkarar</name><name sortKey="Bayoumi, Faisal" sort="Bayoumi, Faisal" uniqKey="Bayoumi F" first="Faisal" last="Bayoumi">Faisal Bayoumi</name><name sortKey="Hashim, Osman" sort="Hashim, Osman" uniqKey="Hashim O" first="Osman" last="Hashim">Osman Hashim</name><name sortKey="Hussien, Raed" sort="Hussien, Raed" uniqKey="Hussien R" first="Raed" last="Hussien">Raed Hussien</name><name sortKey="Qasim, Ibrahim" sort="Qasim, Ibrahim" uniqKey="Qasim I" first="Ibrahim" last="Qasim">Ibrahim Qasim</name><name sortKey="Saleh, Mohamed" sort="Saleh, Mohamed" uniqKey="Saleh M" first="Mohamed" last="Saleh">Mohamed Saleh</name></country><country name="Égypte"><noRegion><name sortKey="Kasem, Samy" sort="Kasem, Samy" uniqKey="Kasem S" first="Samy" last="Kasem">Samy Kasem</name></noRegion><name sortKey="Magouz, Asmaa" sort="Magouz, Asmaa" uniqKey="Magouz A" first="Asmaa" last="Magouz">Asmaa Magouz</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000776 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000776 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:7102711
|texte= The prevalence of Middle East respiratory Syndrome coronavirus (MERS-CoV) infection in livestock and temporal relation to locations and seasons
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:29396257" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |