MersV1, Main, Exploration, bibRecord, 000711

Adiponectin secretion from cardiomyocytes produces canonical multimers and partial co-localization with calsequestrin in junctional SR.

Identifieur interne : 000711 ( Main/Exploration ); précédent : 000710; suivant : 000712

Adiponectin secretion from cardiomyocytes produces canonical multimers and partial co-localization with calsequestrin in junctional SR.

Auteurs : Joanna Solarewicz [États-Unis] ; Amanda Manly [États-Unis] ; Stephanie Kokoszka [États-Unis] ; Naama Sleiman [États-Unis] ; Todd Leff [États-Unis] ; Steven Cala [États-Unis]

Source :

Molecular and cellular biochemistry [ 1573-4919 ] ; 2019.

RBID : pubmed:30919218

Descripteurs français

KwdFr :
- Adiponectine (métabolisme), Animaux, Calséquestrine (métabolisme), Cellules COS, Cellules HEK293, Humains, Multimérisation de protéines, Myocytes cardiaques (métabolisme), Rat Sprague-Dawley, Rats, Réticulum sarcoplasmique (métabolisme), Transport de protéines.
MESH :
- métabolisme : Adiponectine, Calséquestrine, Myocytes cardiaques, Réticulum sarcoplasmique.
- Animaux, Cellules COS, Cellules HEK293, Humains, Multimérisation de protéines, Rat Sprague-Dawley, Rats, Transport de protéines.

English descriptors

KwdEn :
- Adiponectin (metabolism), Animals, COS Cells, Calsequestrin (metabolism), Chlorocebus aethiops, HEK293 Cells, Humans, Myocytes, Cardiac (metabolism), Protein Multimerization, Protein Transport, Rats, Rats, Sprague-Dawley, Sarcoplasmic Reticulum (metabolism).
MESH :
- chemical , metabolism : Adiponectin, Calsequestrin.
- metabolism : Myocytes, Cardiac, Sarcoplasmic Reticulum.
- Animals, COS Cells, Chlorocebus aethiops, HEK293 Cells, Humans, Protein Multimerization, Protein Transport, Rats, Rats, Sprague-Dawley.

Abstract

Adiponectin (ADN) is an abundant protein in serum, secreted by adipocytes, that acts as a signal for fat metabolism. It is marked by a complex molecular structure that results from processes within the secretory pathway, producing a canonical set of multimers. ADN may also be secreted from cardiomyocytes, where a unique sarcomeric endoplasmic/sarcoplasmic reticulum (ER/SR) substructure has been characterized primarily for its Ca handling. We expressed ADN in cultured primary adult cardiomyocytes and nonmuscle (COS) cells. After 48 h of ADN expression by adenovirus treatment, roughly half of synthesized ADN was secreted from cardiomyocytes, and half was still in-transit within inner membrane compartments, similar to COS cells. Cardiomyocytes and COS cells both produced ADN in the three canonical forms: trimers, hexamers, and 18-mers. Higher rates of secretion occurred for higher-molecular weight multimers, especially 18-mers. The highest levels of ADN protein, whether in transit or secreted, were present as trimers and hexamers. In nonmuscle cell lines, ADN trafficked through ER and Golgi compartments as expected. In contrast, ADN in primary adult cardiomyocytes populated ER/SR tubules along the edges of sarcomeres that emanated from nuclear surfaces. Prominent co-localization of ADN occurred with calsequestrin, a marker of junctional SR, the Ca²⁺-release compartment of the cell. The early steps in ADN trafficking re-trace those recently described for newly made junctional SR proteins, involving a nuclear envelope (NE) translocation into SR tubules that are oriented along sarcolemmal transverse (T)-tubules (NEST pathway).

DOI: 10.1007/s11010-019-03524-9
PubMed: 30919218

Affiliations:

États-Unis

Le document en format XML

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<term>Chlorocebus aethiops</term>
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<term>Humans</term>
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<term>Calséquestrine (métabolisme)</term>
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<term>Cellules HEK293</term>
<term>Humains</term>
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<term>Myocytes cardiaques (métabolisme)</term>
<term>Rat Sprague-Dawley</term>
<term>Rats</term>
<term>Réticulum sarcoplasmique (métabolisme)</term>
<term>Transport de protéines</term>
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<term>Sarcoplasmic Reticulum</term>
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<term>Calséquestrine</term>
<term>Myocytes cardiaques</term>
<term>Réticulum sarcoplasmique</term>
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<term>COS Cells</term>
<term>Chlorocebus aethiops</term>
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<term>Protein Transport</term>
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<term>Cellules COS</term>
<term>Cellules HEK293</term>
<term>Humains</term>
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<term>Rat Sprague-Dawley</term>
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<front><div type="abstract" xml:lang="en">Adiponectin (ADN) is an abundant protein in serum, secreted by adipocytes, that acts as a signal for fat metabolism. It is marked by a complex molecular structure that results from processes within the secretory pathway, producing a canonical set of multimers. ADN may also be secreted from cardiomyocytes, where a unique sarcomeric endoplasmic/sarcoplasmic reticulum (ER/SR) substructure has been characterized primarily for its Ca handling. We expressed ADN in cultured primary adult cardiomyocytes and nonmuscle (COS) cells. After 48 h of ADN expression by adenovirus treatment, roughly half of synthesized ADN was secreted from cardiomyocytes, and half was still in-transit within inner membrane compartments, similar to COS cells. Cardiomyocytes and COS cells both produced ADN in the three canonical forms: trimers, hexamers, and 18-mers. Higher rates of secretion occurred for higher-molecular weight multimers, especially 18-mers. The highest levels of ADN protein, whether in transit or secreted, were present as trimers and hexamers. In nonmuscle cell lines, ADN trafficked through ER and Golgi compartments as expected. In contrast, ADN in primary adult cardiomyocytes populated ER/SR tubules along the edges of sarcomeres that emanated from nuclear surfaces. Prominent co-localization of ADN occurred with calsequestrin, a marker of junctional SR, the Ca<sup>2+</sup>
-release compartment of the cell. The early steps in ADN trafficking re-trace those recently described for newly made junctional SR proteins, involving a nuclear envelope (NE) translocation into SR tubules that are oriented along sarcolemmal transverse (T)-tubules (NEST pathway).</div>
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Serveur d'exploration MERS

Adiponectin secretion from cardiomyocytes produces canonical multimers and partial co-localization with calsequestrin in junctional SR.

Adiponectin secretion from cardiomyocytes produces canonical multimers and partial co-localization with calsequestrin in junctional SR.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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