Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex
Identifieur interne : 000562 ( Main/Exploration ); précédent : 000561; suivant : 000563Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex
Auteurs : Byoung Kwon Park [Corée du Sud] ; Sony Maharjan [Corée du Sud] ; Su In Lee [Corée du Sud] ; Jinsoo Kim [Corée du Sud] ; Joon-Yong Bae [Corée du Sud] ; Man-Seong Park [Corée du Sud] ; Hyung-Joo Kwon [Corée du Sud]Source :
- BMB Reports [ 1976-6696 ] ; 2019.
Descripteurs français
- KwdFr :
- ADN, Animaux, Anticorps monoclonaux (isolement et purification), Cellules Vero, Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité), Glycoprotéine de spicule des coronavirus (immunologie), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Ilots CpG (immunologie), Liaison aux protéines, Liposomes, Peptides (immunologie), Souris, Épitopes (immunologie).
- MESH :
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Ilots CpG, Peptides, Épitopes.
- isolement et purification : Anticorps monoclonaux.
- métabolisme : Glycoprotéine de spicule des coronavirus.
- pathogénicité : Coronavirus du syndrome respiratoire du Moyen-Orient.
- ADN, Animaux, Cellules Vero, Humains, Liaison aux protéines, Liposomes, Souris.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (isolation & purification), Chlorocebus aethiops, CpG Islands (immunology), DNA, Epitopes (immunology), Humans, Liposomes, Mice, Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (pathogenicity), Peptides (immunology), Protein Binding, Spike Glycoprotein, Coronavirus (immunology), Spike Glycoprotein, Coronavirus (metabolism), Vero Cells.
- MESH :
- chemical , immunology : Epitopes, Peptides, Spike Glycoprotein, Coronavirus.
- chemical , isolation & purification : Antibodies, Monoclonal.
- immunology : CpG Islands, Middle East Respiratory Syndrome Coronavirus.
- chemical , metabolism : Spike Glycoprotein, Coronavirus.
- pathogenicity : Middle East Respiratory Syndrome Coronavirus.
- Animals, Chlorocebus aethiops, DNA, Humans, Liposomes, Mice, Protein Binding, Vero Cells.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) uses the spike (S) glycoprotein to recognize and enter target cells. In this study, we selected two epitope peptide sequences within the receptor binding domain (RBD) of the MERS-CoV S protein. We used a complex consisting of the epitope peptide of the MERS-CoV S protein and CpG-DNA encapsulated in liposome complex to immunize mice, and produced the monoclonal antibodies 506-2G10G5 and 492-1G10E4E2. The western blotting data showed that both monoclonal antibodies detected the S protein and immunoprecipitated the native form of the S protein. Indirect immunofluorescence and confocal analysis suggested strong reactivity of the antibodies towards the S protein of MERS-CoV virus infected Vero cells. Furthermore, the 506-2G10G5 monoclonal antibody significantly reduced plaque formation in MERS-CoV infected Vero cells compared to normal mouse IgG and 492-1G10E4E2. Thus, we successfully produced a monoclonal antibody directed against the RBD domain of the S protein which could be used in the development of diagnostics and therapeutic applications in the future.
Url:
DOI: 10.5483/BMBRep.2019.52.6.185
PubMed: 30355437
PubMed Central: 6605520
Affiliations:
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Le document en format XML
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for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Maharjan, Sony" sort="Maharjan, Sony" uniqKey="Maharjan S" first="Sony" last="Maharjan">Sony Maharjan</name><affiliation wicri:level="1"><nlm:aff id="af1-bmb-52-397">Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Lee, Su In" sort="Lee, Su In" uniqKey="Lee S" first="Su In" last="Lee">Su In Lee</name><affiliation wicri:level="1"><nlm:aff id="af1-bmb-52-397">Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Kim, Jinsoo" sort="Kim, Jinsoo" uniqKey="Kim J" first="Jinsoo" last="Kim">Jinsoo Kim</name><affiliation wicri:level="1"><nlm:aff id="af2-bmb-52-397">Department of Microbiology, College of Medicine, Hallym University, Chuncheon 24252,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Bae, Joon Yong" sort="Bae, Joon Yong" uniqKey="Bae J" first="Joon-Yong" last="Bae">Joon-Yong Bae</name><affiliation wicri:level="1"><nlm:aff id="af3-bmb-52-397">Department of Microbiology, College of Medicine, and the Institute for Viral Diseases, Korea University, Seoul 02841,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Park, Man Seong" sort="Park, Man Seong" uniqKey="Park M" first="Man-Seong" last="Park">Man-Seong Park</name><affiliation wicri:level="1"><nlm:aff id="af3-bmb-52-397">Department of Microbiology, College of Medicine, and the Institute for Viral Diseases, Korea University, Seoul 02841,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Kwon, Hyung Joo" sort="Kwon, Hyung Joo" uniqKey="Kwon H" first="Hyung-Joo" last="Kwon">Hyung-Joo Kwon</name><affiliation wicri:level="1"><nlm:aff id="af1-bmb-52-397">Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="af2-bmb-52-397">Department of Microbiology, College of Medicine, Hallym University, Chuncheon 24252,<country>Korea</country></nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">BMB Reports</title><idno type="ISSN">1976-6696</idno><idno type="eISSN">1976-670X</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (isolation & purification)</term><term>Chlorocebus aethiops</term><term>CpG Islands (immunology)</term><term>DNA</term><term>Epitopes (immunology)</term><term>Humans</term><term>Liposomes</term><term>Mice</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Middle East Respiratory Syndrome Coronavirus (pathogenicity)</term><term>Peptides (immunology)</term><term>Protein Binding</term><term>Spike Glycoprotein, Coronavirus (immunology)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN</term><term>Animaux</term><term>Anticorps monoclonaux (isolement et purification)</term><term>Cellules Vero</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité)</term><term>Glycoprotéine de spicule des coronavirus (immunologie)</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Humains</term><term>Ilots CpG (immunologie)</term><term>Liaison aux protéines</term><term>Liposomes</term><term>Peptides (immunologie)</term><term>Souris</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Epitopes</term><term>Peptides</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Ilots CpG</term><term>Peptides</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CpG Islands</term><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>DNA</term><term>Humans</term><term>Liposomes</term><term>Mice</term><term>Protein Binding</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>Animaux</term><term>Cellules Vero</term><term>Humains</term><term>Liaison aux protéines</term><term>Liposomes</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Middle East respiratory syndrome coronavirus (MERS-CoV) uses the spike (S) glycoprotein to recognize and enter target cells. In this study, we selected two epitope peptide sequences within the receptor binding domain (RBD) of the MERS-CoV S protein. We used a complex consisting of the epitope peptide of the MERS-CoV S protein and CpG-DNA encapsulated in liposome complex to immunize mice, and produced the monoclonal antibodies 506-2G10G5 and 492-1G10E4E2. The western blotting data showed that both monoclonal antibodies detected the S protein and immunoprecipitated the native form of the S protein. Indirect immunofluorescence and confocal analysis suggested strong reactivity of the antibodies towards the S protein of MERS-CoV virus infected Vero cells. Furthermore, the 506-2G10G5 monoclonal antibody significantly reduced plaque formation in MERS-CoV infected Vero cells compared to normal mouse IgG and 492-1G10E4E2. Thus, we successfully produced a monoclonal antibody directed against the RBD domain of the S protein which could be used in the development of diagnostics and therapeutic applications in the future.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zumla, A" uniqKey="Zumla A">A Zumla</name></author><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author><author><name sortKey="Azhar, Ei" uniqKey="Azhar E">EI Azhar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y Yang</name></author><author><name sortKey="Zhou, Y" uniqKey="Zhou Y">Y Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mackay, Im" uniqKey="Mackay I">IM Mackay</name></author><author><name sortKey="Arden, Ke" uniqKey="Arden K">KE 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uniqKey="Maharjan S">S Maharjan</name></author><author><name sortKey="Park, Bk" uniqKey="Park B">BK Park</name></author><author><name sortKey="Lee, Si" uniqKey="Lee S">SI Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maharjan, S" uniqKey="Maharjan S">S Maharjan</name></author><author><name sortKey="Park, Bk" uniqKey="Park B">BK Park</name></author><author><name sortKey="Lee, Si" uniqKey="Lee S">SI Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jo, Hs" uniqKey="Jo H">HS Jo</name></author><author><name sortKey="Eum, Ws" uniqKey="Eum W">WS Eum</name></author><author><name sortKey="Park, Ey" uniqKey="Park E">EY Park</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, Ew" uniqKey="Lee E">EW Lee</name></author><author><name sortKey="Oh, W" uniqKey="Oh W">W Oh</name></author><author><name sortKey="Song, Hp" uniqKey="Song H">HP Song</name></author><author><name sortKey="Kim, Wk" uniqKey="Kim W">WK Kim</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Park, Byoung Kwon" sort="Park, Byoung Kwon" uniqKey="Park B" first="Byoung Kwon" last="Park">Byoung Kwon Park</name></noRegion><name sortKey="Bae, Joon Yong" sort="Bae, Joon Yong" uniqKey="Bae J" first="Joon-Yong" last="Bae">Joon-Yong Bae</name><name sortKey="Kim, Jinsoo" sort="Kim, Jinsoo" uniqKey="Kim J" first="Jinsoo" last="Kim">Jinsoo Kim</name><name sortKey="Kwon, Hyung Joo" sort="Kwon, Hyung Joo" uniqKey="Kwon H" first="Hyung-Joo" last="Kwon">Hyung-Joo Kwon</name><name sortKey="Kwon, Hyung Joo" sort="Kwon, Hyung Joo" uniqKey="Kwon H" first="Hyung-Joo" last="Kwon">Hyung-Joo Kwon</name><name sortKey="Lee, Su In" sort="Lee, Su In" uniqKey="Lee S" first="Su In" last="Lee">Su In Lee</name><name sortKey="Maharjan, Sony" sort="Maharjan, Sony" uniqKey="Maharjan S" first="Sony" last="Maharjan">Sony Maharjan</name><name sortKey="Park, Man Seong" sort="Park, Man Seong" uniqKey="Park M" first="Man-Seong" last="Park">Man-Seong Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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