Hole Migration in Telomere‐Based Oligonucleotide Anions and G‐Quadruplexes
Identifieur interne : 000547 ( Main/Exploration ); précédent : 000546; suivant : 000548Hole Migration in Telomere‐Based Oligonucleotide Anions and G‐Quadruplexes
Auteurs : Wen Li ; Edita Mjekiqi ; Wessel Douma ; Xin Wang ; Oksana Kavatsyuk ; Ronnie Hoekstra ; Jean-Christophe Poully ; Thomas SchlathölterSource :
- Chemistry (Weinheim an Der Bergstrasse, Germany) [ 0947-6539 ] ; 2019.
Abstract
Vacuum ultraviolet photoionization of a gas‐phase oligonucleotide anion leads to the formation of a valence hole. This hole migrates towards an energetically favorable site where it can weaken bonds and ultimately lead to bond cleavage. We have studied Vacuum UV photoionization of deprotonated oligonucleotides containing the human telomere sequence dTTAGGG and G‐quadruplex structures consisting of four dTGGGGT single strands, stabilized by NH4+ counter ions. The oligonucleotide and G‐quadruplex anions were confined in a radiofrequency ion trap, interfaced with a synchrotron beamline and the photofragmentation was studied using time‐of‐flight mass spectrometry. Oligonucleotide 12‐mers containing the 5'‐TTAGGG sequence were found to predominantly break in the GGG region, whereas no selective bond cleavage region was observed for the reversed 5'‐GGGATT sequence. For G‐quadruplex structures, fragmentation was quenched and mostly non‐dissociative single and double electron removal was observed.
Url:
DOI: 10.1002/chem.201904105
PubMed: 31614016
PubMed Central: 6972685
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000B67
- to stream Pmc, to step Curation: 000B67
- to stream Pmc, to step Checkpoint: 000290
- to stream PubMed, to step Corpus: 000397
- to stream PubMed, to step Curation: 000397
- to stream PubMed, to step Checkpoint: 000536
- to stream Ncbi, to step Merge: 002365
- to stream Ncbi, to step Curation: 002365
- to stream Ncbi, to step Checkpoint: 002365
- to stream Main, to step Merge: 000550
- to stream Main, to step Curation: 000547
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Hole Migration in Telomere‐Based Oligonucleotide Anions and G‐Quadruplexes</title>
<author><name sortKey="Li, Wen" sort="Li, Wen" uniqKey="Li W" first="Wen" last="Li">Wen Li</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mjekiqi, Edita" sort="Mjekiqi, Edita" uniqKey="Mjekiqi E" first="Edita" last="Mjekiqi">Edita Mjekiqi</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Douma, Wessel" sort="Douma, Wessel" uniqKey="Douma W" first="Wessel" last="Douma">Wessel Douma</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Xin" sort="Wang, Xin" uniqKey="Wang X" first="Xin" last="Wang">Xin Wang</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kavatsyuk, Oksana" sort="Kavatsyuk, Oksana" uniqKey="Kavatsyuk O" first="Oksana" last="Kavatsyuk">Oksana Kavatsyuk</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="chem201904105-aff-0002"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hoekstra, Ronnie" sort="Hoekstra, Ronnie" uniqKey="Hoekstra R" first="Ronnie" last="Hoekstra">Ronnie Hoekstra</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Poully, Jean Hristophe" sort="Poully, Jean Hristophe" uniqKey="Poully J" first="Jean-Christophe" last="Poully">Jean-Christophe Poully</name>
<affiliation><nlm:aff id="chem201904105-aff-0003"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Schlatholter, Thomas" sort="Schlatholter, Thomas" uniqKey="Schlatholter T" first="Thomas" last="Schlathölter">Thomas Schlathölter</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">31614016</idno>
<idno type="pmc">6972685</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6972685</idno>
<idno type="RBID">PMC:6972685</idno>
<idno type="doi">10.1002/chem.201904105</idno>
<date when="2019">2019</date>
<idno type="wicri:Area/Pmc/Corpus">000B67</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B67</idno>
<idno type="wicri:Area/Pmc/Curation">000B67</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B67</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000290</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000290</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:31614016</idno>
<idno type="wicri:Area/PubMed/Corpus">000397</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000397</idno>
<idno type="wicri:Area/PubMed/Curation">000397</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000397</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000536</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000536</idno>
<idno type="wicri:Area/Ncbi/Merge">002365</idno>
<idno type="wicri:Area/Ncbi/Curation">002365</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002365</idno>
<idno type="wicri:doubleKey">0947-6539:2019:Li W:hole:migration:in</idno>
<idno type="wicri:Area/Main/Merge">000550</idno>
<idno type="wicri:Area/Main/Curation">000547</idno>
<idno type="wicri:Area/Main/Exploration">000547</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Hole Migration in Telomere‐Based Oligonucleotide Anions and G‐Quadruplexes</title>
<author><name sortKey="Li, Wen" sort="Li, Wen" uniqKey="Li W" first="Wen" last="Li">Wen Li</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mjekiqi, Edita" sort="Mjekiqi, Edita" uniqKey="Mjekiqi E" first="Edita" last="Mjekiqi">Edita Mjekiqi</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Douma, Wessel" sort="Douma, Wessel" uniqKey="Douma W" first="Wessel" last="Douma">Wessel Douma</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Xin" sort="Wang, Xin" uniqKey="Wang X" first="Xin" last="Wang">Xin Wang</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kavatsyuk, Oksana" sort="Kavatsyuk, Oksana" uniqKey="Kavatsyuk O" first="Oksana" last="Kavatsyuk">Oksana Kavatsyuk</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="chem201904105-aff-0002"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hoekstra, Ronnie" sort="Hoekstra, Ronnie" uniqKey="Hoekstra R" first="Ronnie" last="Hoekstra">Ronnie Hoekstra</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Poully, Jean Hristophe" sort="Poully, Jean Hristophe" uniqKey="Poully J" first="Jean-Christophe" last="Poully">Jean-Christophe Poully</name>
<affiliation><nlm:aff id="chem201904105-aff-0003"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Schlatholter, Thomas" sort="Schlatholter, Thomas" uniqKey="Schlatholter T" first="Thomas" last="Schlathölter">Thomas Schlathölter</name>
<affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Chemistry (Weinheim an Der Bergstrasse, Germany)</title>
<idno type="ISSN">0947-6539</idno>
<idno type="eISSN">1521-3765</idno>
<imprint><date when="2019">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>Vacuum ultraviolet photoionization of a gas‐phase oligonucleotide anion leads to the formation of a valence hole. This hole migrates towards an energetically favorable site where it can weaken bonds and ultimately lead to bond cleavage. We have studied Vacuum UV photoionization of deprotonated oligonucleotides containing the human telomere sequence dTTAGGG and G‐quadruplex structures consisting of four dTGGGGT single strands, stabilized by NH<sub>4</sub>
<sup>+</sup>
counter ions. The oligonucleotide and G‐quadruplex anions were confined in a radiofrequency ion trap, interfaced with a synchrotron beamline and the photofragmentation was studied using time‐of‐flight mass spectrometry. Oligonucleotide 12‐mers containing the 5'‐TTAGGG sequence were found to predominantly break in the GGG region, whereas no selective bond cleavage region was observed for the reversed 5'‐GGGATT sequence. For G‐quadruplex structures, fragmentation was quenched and mostly non‐dissociative single and double electron removal was observed.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Douma, Wessel" sort="Douma, Wessel" uniqKey="Douma W" first="Wessel" last="Douma">Wessel Douma</name>
<name sortKey="Hoekstra, Ronnie" sort="Hoekstra, Ronnie" uniqKey="Hoekstra R" first="Ronnie" last="Hoekstra">Ronnie Hoekstra</name>
<name sortKey="Kavatsyuk, Oksana" sort="Kavatsyuk, Oksana" uniqKey="Kavatsyuk O" first="Oksana" last="Kavatsyuk">Oksana Kavatsyuk</name>
<name sortKey="Li, Wen" sort="Li, Wen" uniqKey="Li W" first="Wen" last="Li">Wen Li</name>
<name sortKey="Mjekiqi, Edita" sort="Mjekiqi, Edita" uniqKey="Mjekiqi E" first="Edita" last="Mjekiqi">Edita Mjekiqi</name>
<name sortKey="Poully, Jean Hristophe" sort="Poully, Jean Hristophe" uniqKey="Poully J" first="Jean-Christophe" last="Poully">Jean-Christophe Poully</name>
<name sortKey="Schlatholter, Thomas" sort="Schlatholter, Thomas" uniqKey="Schlatholter T" first="Thomas" last="Schlathölter">Thomas Schlathölter</name>
<name sortKey="Wang, Xin" sort="Wang, Xin" uniqKey="Wang X" first="Xin" last="Wang">Xin Wang</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000547 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000547 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Exploration |type= RBID |clé= PMC:6972685 |texte= Hole Migration in Telomere‐Based Oligonucleotide Anions and G‐Quadruplexes }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31614016" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |