Hole Migration in Telomere‐Based Oligonucleotide Anions and G‐Quadruplexes
Identifieur interne : 000547 ( Main/Exploration ); précédent : 000546; suivant : 000548Hole Migration in Telomere‐Based Oligonucleotide Anions and G‐Quadruplexes
Auteurs : Wen Li ; Edita Mjekiqi ; Wessel Douma ; Xin Wang ; Oksana Kavatsyuk ; Ronnie Hoekstra ; Jean-Christophe Poully ; Thomas SchlathölterSource :
- Chemistry (Weinheim an Der Bergstrasse, Germany) [ 0947-6539 ] ; 2019.
Abstract
Vacuum ultraviolet photoionization of a gas‐phase oligonucleotide anion leads to the formation of a valence hole. This hole migrates towards an energetically favorable site where it can weaken bonds and ultimately lead to bond cleavage. We have studied Vacuum UV photoionization of deprotonated oligonucleotides containing the human telomere sequence dTTAGGG and G‐quadruplex structures consisting of four dTGGGGT single strands, stabilized by NH4+ counter ions. The oligonucleotide and G‐quadruplex anions were confined in a radiofrequency ion trap, interfaced with a synchrotron beamline and the photofragmentation was studied using time‐of‐flight mass spectrometry. Oligonucleotide 12‐mers containing the 5'‐TTAGGG sequence were found to predominantly break in the GGG region, whereas no selective bond cleavage region was observed for the reversed 5'‐GGGATT sequence. For G‐quadruplex structures, fragmentation was quenched and mostly non‐dissociative single and double electron removal was observed.
Url:
DOI: 10.1002/chem.201904105
PubMed: 31614016
PubMed Central: 6972685
Affiliations:
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Hoekstra</name><affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff></affiliation></author><author><name sortKey="Poully, Jean Hristophe" sort="Poully, Jean Hristophe" uniqKey="Poully J" first="Jean-Christophe" last="Poully">Jean-Christophe Poully</name><affiliation><nlm:aff id="chem201904105-aff-0003"></nlm:aff></affiliation></author><author><name sortKey="Schlatholter, Thomas" sort="Schlatholter, Thomas" uniqKey="Schlatholter T" first="Thomas" last="Schlathölter">Thomas Schlathölter</name><affiliation><nlm:aff id="chem201904105-aff-0001"></nlm:aff></affiliation></author></analytic><series><title level="j">Chemistry (Weinheim an Der Bergstrasse, Germany)</title><idno type="ISSN">0947-6539</idno><idno type="eISSN">1521-3765</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>Vacuum ultraviolet photoionization of a gas‐phase oligonucleotide anion leads to the formation of a valence hole. This hole migrates towards an energetically favorable site where it can weaken bonds and ultimately lead to bond cleavage. We have studied Vacuum UV photoionization of deprotonated oligonucleotides containing the human telomere sequence dTTAGGG and G‐quadruplex structures consisting of four dTGGGGT single strands, stabilized by NH<sub>4</sub><sup>+</sup> counter ions. The oligonucleotide and G‐quadruplex anions were confined in a radiofrequency ion trap, interfaced with a synchrotron beamline and the photofragmentation was studied using time‐of‐flight mass spectrometry. Oligonucleotide 12‐mers containing the 5'‐TTAGGG sequence were found to predominantly break in the GGG region, whereas no selective bond cleavage region was observed for the reversed 5'‐GGGATT sequence. For G‐quadruplex structures, fragmentation was quenched and mostly non‐dissociative single and double electron removal was observed.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Douma, Wessel" sort="Douma, Wessel" uniqKey="Douma W" first="Wessel" last="Douma">Wessel Douma</name><name sortKey="Hoekstra, Ronnie" sort="Hoekstra, Ronnie" uniqKey="Hoekstra R" first="Ronnie" last="Hoekstra">Ronnie Hoekstra</name><name sortKey="Kavatsyuk, Oksana" sort="Kavatsyuk, Oksana" uniqKey="Kavatsyuk O" first="Oksana" last="Kavatsyuk">Oksana Kavatsyuk</name><name sortKey="Li, Wen" sort="Li, Wen" uniqKey="Li W" first="Wen" last="Li">Wen Li</name><name sortKey="Mjekiqi, Edita" sort="Mjekiqi, Edita" uniqKey="Mjekiqi E" first="Edita" last="Mjekiqi">Edita Mjekiqi</name><name sortKey="Poully, Jean Hristophe" sort="Poully, Jean Hristophe" uniqKey="Poully J" first="Jean-Christophe" last="Poully">Jean-Christophe Poully</name><name sortKey="Schlatholter, Thomas" sort="Schlatholter, Thomas" uniqKey="Schlatholter T" first="Thomas" last="Schlathölter">Thomas Schlathölter</name><name sortKey="Wang, Xin" sort="Wang, Xin" uniqKey="Wang X" first="Xin" last="Wang">Xin Wang</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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