Multiplex Solid-Phase Melt Curve Analysis for the Point-of-Care Detection of HIV-1 Drug Resistance.
Identifieur interne : 000451 ( Main/Exploration ); précédent : 000450; suivant : 000452Multiplex Solid-Phase Melt Curve Analysis for the Point-of-Care Detection of HIV-1 Drug Resistance.
Auteurs : Dana S. Clutter [États-Unis] ; Gelareh Mazarei [États-Unis] ; Ruma Sinha [États-Unis] ; Justen Manasa [Zimbabwe] ; Janin Nouhin [États-Unis] ; Ellen Laprade [États-Unis] ; Sara Bolouki [États-Unis] ; Philip L. Tzou [États-Unis] ; Jessica Hannita-Hui [États-Unis] ; Malaya K. Sahoo [États-Unis] ; Peter Kuimelis [États-Unis] ; Robert G. Kuimelis [États-Unis] ; Benjamin A. Pinsky [États-Unis] ; Gary K. Schoolnik [États-Unis] ; Arjang Hassibi [États-Unis] ; Robert W. Shafer [États-Unis]Source :
- The Journal of molecular diagnostics : JMD [ 1943-7811 ] ; 2019.
Abstract
A point-of-care HIV-1 genotypic resistance assay that could be performed during a clinic visit would enable care providers to make informed treatment decisions for patients starting therapy or experiencing virologic failure on therapy. The main challenge for such an assay is the genetic variability at and surrounding each drug-resistance mutation (DRM). We analyzed a database of diverse global HIV sequences and used thermodynamic simulations to design an array of surface-bound oligonucleotide probe sets with each set sharing distinct 5' and 3' flanking sequences but having different centrally located nucleotides complementary to six codons at HIV-1 DRM reverse transcriptase position 103: AAA, AAC, AAG, AAT, AGA, and AGC. We then performed in vitro experiments using 80-mer oligonucleotides and PCR-amplified DNA from clinical plasma HIV-1 samples and culture supernatants that contained subtype A, B, C, D, CRF01_AE, and CRF02_AG viruses. Multiplexed solid-phase melt curve analysis discriminated perfectly among each of the six reported reverse transcriptase position 103 codons in both 80-mers and clinical samples. The sensitivity and specificity for detecting targets that contained AAC mixed with targets that contained AAA were >98% when AAC was present at a proportion of ≥10%. Multiplexed solid-phase melt curve analysis is a promising approach for developing point-of-care assays to distinguish between different codons in genetically variable regions such as those surrounding HIV-1 DRMs.
DOI: 10.1016/j.jmoldx.2019.02.005
PubMed: 31026601
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000554
- to stream PubMed, to step Curation: 000554
- to stream PubMed, to step Checkpoint: 000447
- to stream Ncbi, to step Merge: 002203
- to stream Ncbi, to step Curation: 002203
- to stream Ncbi, to step Checkpoint: 002203
- to stream Main, to step Merge: 000454
- to stream Main, to step Curation: 000451
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multiplex Solid-Phase Melt Curve Analysis for the Point-of-Care Detection of HIV-1 Drug Resistance.</title><author><name sortKey="Clutter, Dana S" sort="Clutter, Dana S" uniqKey="Clutter D" first="Dana S" last="Clutter">Dana S. Clutter</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Mazarei, Gelareh" sort="Mazarei, Gelareh" uniqKey="Mazarei G" first="Gelareh" last="Mazarei">Gelareh Mazarei</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Sinha, Ruma" sort="Sinha, Ruma" uniqKey="Sinha R" first="Ruma" last="Sinha">Ruma Sinha</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Manasa, Justen" sort="Manasa, Justen" uniqKey="Manasa J" first="Justen" last="Manasa">Justen Manasa</name><affiliation wicri:level="1"><nlm:affiliation>African Institute of Biomedical Science and Technology, Harare, Zimbabwe.</nlm:affiliation><country xml:lang="fr">Zimbabwe</country><wicri:regionArea>African Institute of Biomedical Science and Technology, Harare</wicri:regionArea><wicri:noRegion>Harare</wicri:noRegion></affiliation></author><author><name sortKey="Nouhin, Janin" sort="Nouhin, Janin" uniqKey="Nouhin J" first="Janin" last="Nouhin">Janin Nouhin</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Laprade, Ellen" sort="Laprade, Ellen" uniqKey="Laprade E" first="Ellen" last="Laprade">Ellen Laprade</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Bolouki, Sara" sort="Bolouki, Sara" uniqKey="Bolouki S" first="Sara" last="Bolouki">Sara Bolouki</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Tzou, Philip L" sort="Tzou, Philip L" uniqKey="Tzou P" first="Philip L" last="Tzou">Philip L. Tzou</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Hannita Hui, Jessica" sort="Hannita Hui, Jessica" uniqKey="Hannita Hui J" first="Jessica" last="Hannita-Hui">Jessica Hannita-Hui</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Sahoo, Malaya K" sort="Sahoo, Malaya K" uniqKey="Sahoo M" first="Malaya K" last="Sahoo">Malaya K. Sahoo</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Kuimelis, Peter" sort="Kuimelis, Peter" uniqKey="Kuimelis P" first="Peter" last="Kuimelis">Peter Kuimelis</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Kuimelis, Robert G" sort="Kuimelis, Robert G" uniqKey="Kuimelis R" first="Robert G" last="Kuimelis">Robert G. Kuimelis</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Pinsky, Benjamin A" sort="Pinsky, Benjamin A" uniqKey="Pinsky B" first="Benjamin A" last="Pinsky">Benjamin A. Pinsky</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California; Department of Pathology, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California; Department of Pathology, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Schoolnik, Gary K" sort="Schoolnik, Gary K" uniqKey="Schoolnik G" first="Gary K" last="Schoolnik">Gary K. Schoolnik</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Hassibi, Arjang" sort="Hassibi, Arjang" uniqKey="Hassibi A" first="Arjang" last="Hassibi">Arjang Hassibi</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Shafer, Robert W" sort="Shafer, Robert W" uniqKey="Shafer R" first="Robert W" last="Shafer">Robert W. Shafer</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California. Electronic address: rshafer@stanford.edu.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31026601</idno><idno type="pmid">31026601</idno><idno type="doi">10.1016/j.jmoldx.2019.02.005</idno><idno type="wicri:Area/PubMed/Corpus">000554</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000554</idno><idno type="wicri:Area/PubMed/Curation">000554</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000554</idno><idno type="wicri:Area/PubMed/Checkpoint">000447</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000447</idno><idno type="wicri:Area/Ncbi/Merge">002203</idno><idno type="wicri:Area/Ncbi/Curation">002203</idno><idno type="wicri:Area/Ncbi/Checkpoint">002203</idno><idno type="wicri:Area/Main/Merge">000454</idno><idno type="wicri:Area/Main/Curation">000451</idno><idno type="wicri:Area/Main/Exploration">000451</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Multiplex Solid-Phase Melt Curve Analysis for the Point-of-Care Detection of HIV-1 Drug Resistance.</title><author><name sortKey="Clutter, Dana S" sort="Clutter, Dana S" uniqKey="Clutter D" first="Dana S" last="Clutter">Dana S. Clutter</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Mazarei, Gelareh" sort="Mazarei, Gelareh" uniqKey="Mazarei G" first="Gelareh" last="Mazarei">Gelareh Mazarei</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Sinha, Ruma" sort="Sinha, Ruma" uniqKey="Sinha R" first="Ruma" last="Sinha">Ruma Sinha</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Manasa, Justen" sort="Manasa, Justen" uniqKey="Manasa J" first="Justen" last="Manasa">Justen Manasa</name><affiliation wicri:level="1"><nlm:affiliation>African Institute of Biomedical Science and Technology, Harare, Zimbabwe.</nlm:affiliation><country xml:lang="fr">Zimbabwe</country><wicri:regionArea>African Institute of Biomedical Science and Technology, Harare</wicri:regionArea><wicri:noRegion>Harare</wicri:noRegion></affiliation></author><author><name sortKey="Nouhin, Janin" sort="Nouhin, Janin" uniqKey="Nouhin J" first="Janin" last="Nouhin">Janin Nouhin</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Laprade, Ellen" sort="Laprade, Ellen" uniqKey="Laprade E" first="Ellen" last="Laprade">Ellen Laprade</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Bolouki, Sara" sort="Bolouki, Sara" uniqKey="Bolouki S" first="Sara" last="Bolouki">Sara Bolouki</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Tzou, Philip L" sort="Tzou, Philip L" uniqKey="Tzou P" first="Philip L" last="Tzou">Philip L. Tzou</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Hannita Hui, Jessica" sort="Hannita Hui, Jessica" uniqKey="Hannita Hui J" first="Jessica" last="Hannita-Hui">Jessica Hannita-Hui</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Sahoo, Malaya K" sort="Sahoo, Malaya K" uniqKey="Sahoo M" first="Malaya K" last="Sahoo">Malaya K. Sahoo</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Kuimelis, Peter" sort="Kuimelis, Peter" uniqKey="Kuimelis P" first="Peter" last="Kuimelis">Peter Kuimelis</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Kuimelis, Robert G" sort="Kuimelis, Robert G" uniqKey="Kuimelis R" first="Robert G" last="Kuimelis">Robert G. Kuimelis</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Pinsky, Benjamin A" sort="Pinsky, Benjamin A" uniqKey="Pinsky B" first="Benjamin A" last="Pinsky">Benjamin A. Pinsky</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California; Department of Pathology, Stanford University School of Medicine, Stanford, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California; Department of Pathology, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author><author><name sortKey="Schoolnik, Gary K" sort="Schoolnik, Gary K" uniqKey="Schoolnik G" first="Gary K" last="Schoolnik">Gary K. Schoolnik</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Hassibi, Arjang" sort="Hassibi, Arjang" uniqKey="Hassibi A" first="Arjang" last="Hassibi">Arjang Hassibi</name><affiliation wicri:level="2"><nlm:affiliation>InSilixa Inc., Sunnyvale, California.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>InSilixa Inc., Sunnyvale</wicri:cityArea></affiliation></author><author><name sortKey="Shafer, Robert W" sort="Shafer, Robert W" uniqKey="Shafer R" first="Robert W" last="Shafer">Robert W. Shafer</name><affiliation wicri:level="2"><nlm:affiliation>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California. Electronic address: rshafer@stanford.edu.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford</wicri:cityArea></affiliation></author></analytic><series><title level="j">The Journal of molecular diagnostics : JMD</title><idno type="eISSN">1943-7811</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A point-of-care HIV-1 genotypic resistance assay that could be performed during a clinic visit would enable care providers to make informed treatment decisions for patients starting therapy or experiencing virologic failure on therapy. The main challenge for such an assay is the genetic variability at and surrounding each drug-resistance mutation (DRM). We analyzed a database of diverse global HIV sequences and used thermodynamic simulations to design an array of surface-bound oligonucleotide probe sets with each set sharing distinct 5' and 3' flanking sequences but having different centrally located nucleotides complementary to six codons at HIV-1 DRM reverse transcriptase position 103: AAA, AAC, AAG, AAT, AGA, and AGC. We then performed in vitro experiments using 80-mer oligonucleotides and PCR-amplified DNA from clinical plasma HIV-1 samples and culture supernatants that contained subtype A, B, C, D, CRF01_AE, and CRF02_AG viruses. Multiplexed solid-phase melt curve analysis discriminated perfectly among each of the six reported reverse transcriptase position 103 codons in both 80-mers and clinical samples. The sensitivity and specificity for detecting targets that contained AAC mixed with targets that contained AAA were >98% when AAC was present at a proportion of ≥10%. Multiplexed solid-phase melt curve analysis is a promising approach for developing point-of-care assays to distinguish between different codons in genetically variable regions such as those surrounding HIV-1 DRMs.</div></front></TEI><affiliations><list><country><li>Zimbabwe</li><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Clutter, Dana S" sort="Clutter, Dana S" uniqKey="Clutter D" first="Dana S" last="Clutter">Dana S. Clutter</name></region><name sortKey="Bolouki, Sara" sort="Bolouki, Sara" uniqKey="Bolouki S" first="Sara" last="Bolouki">Sara Bolouki</name><name sortKey="Hannita Hui, Jessica" sort="Hannita Hui, Jessica" uniqKey="Hannita Hui J" first="Jessica" last="Hannita-Hui">Jessica Hannita-Hui</name><name sortKey="Hassibi, Arjang" sort="Hassibi, Arjang" uniqKey="Hassibi A" first="Arjang" last="Hassibi">Arjang Hassibi</name><name sortKey="Kuimelis, Peter" sort="Kuimelis, Peter" uniqKey="Kuimelis P" first="Peter" last="Kuimelis">Peter Kuimelis</name><name sortKey="Kuimelis, Robert G" sort="Kuimelis, Robert G" uniqKey="Kuimelis R" first="Robert G" last="Kuimelis">Robert G. Kuimelis</name><name sortKey="Laprade, Ellen" sort="Laprade, Ellen" uniqKey="Laprade E" first="Ellen" last="Laprade">Ellen Laprade</name><name sortKey="Mazarei, Gelareh" sort="Mazarei, Gelareh" uniqKey="Mazarei G" first="Gelareh" last="Mazarei">Gelareh Mazarei</name><name sortKey="Nouhin, Janin" sort="Nouhin, Janin" uniqKey="Nouhin J" first="Janin" last="Nouhin">Janin Nouhin</name><name sortKey="Pinsky, Benjamin A" sort="Pinsky, Benjamin A" uniqKey="Pinsky B" first="Benjamin A" last="Pinsky">Benjamin A. Pinsky</name><name sortKey="Sahoo, Malaya K" sort="Sahoo, Malaya K" uniqKey="Sahoo M" first="Malaya K" last="Sahoo">Malaya K. Sahoo</name><name sortKey="Schoolnik, Gary K" sort="Schoolnik, Gary K" uniqKey="Schoolnik G" first="Gary K" last="Schoolnik">Gary K. Schoolnik</name><name sortKey="Shafer, Robert W" sort="Shafer, Robert W" uniqKey="Shafer R" first="Robert W" last="Shafer">Robert W. Shafer</name><name sortKey="Sinha, Ruma" sort="Sinha, Ruma" uniqKey="Sinha R" first="Ruma" last="Sinha">Ruma Sinha</name><name sortKey="Tzou, Philip L" sort="Tzou, Philip L" uniqKey="Tzou P" first="Philip L" last="Tzou">Philip L. Tzou</name></country><country name="Zimbabwe"><noRegion><name sortKey="Manasa, Justen" sort="Manasa, Justen" uniqKey="Manasa J" first="Justen" last="Manasa">Justen Manasa</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000451 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000451 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:31026601
|texte= Multiplex Solid-Phase Melt Curve Analysis for the Point-of-Care Detection of HIV-1 Drug Resistance.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31026601" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |