MersV1, Main, Exploration, bibRecord, 000416

Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA

Identifieur interne : 000416 ( Main/Exploration ); précédent : 000415; suivant : 000417

Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA

Auteurs :

Source :

MERS Coronavirus ; 2019.

RBID : PMC:7122216

Abstract

Indirect enzyme-linked immunosorbent assay (ELISA) enables detection and quantification of antigen-specific antibodies in biological samples such as human or animal sera. Most current MERS-CoV serological assays such as neutralization, immunofluorescence, or protein microarray rely on handling of live MERS-CoV in high containment laboratories, highly trained personnel as well as the need for expensive and special equipment and reagents representing a hurdle for most laboratories especially when resources are limited. In this chapter, we describe a validated and optimized indirect ELISA protocol based on recombinant S1 subunit (amino acids 1–725) of MERS-CoV for qualitative and quantitative determination of MERS-CoV-binding antibodies.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122216

DOI: 10.1007/978-1-0716-0211-9_11
PubMed: 31883093
PubMed Central: 7122216

Affiliations:

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Serveur d'exploration MERS

Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA

Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA

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Abstract

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