Camelid type I interferons: Identification and functional characterization of interferon alpha from the dromedary camel (Camelus dromedarius).
Identifieur interne : 000267 ( Main/Exploration ); précédent : 000266; suivant : 000268Camelid type I interferons: Identification and functional characterization of interferon alpha from the dromedary camel (Camelus dromedarius).
Auteurs : Avinash Premraj [Émirats arabes unis] ; Abi George Aleyas [Émirats arabes unis] ; Binita Nautiyal [Émirats arabes unis] ; Thaha Jamal Rasool [Émirats arabes unis]Source :
- Molecular immunology [ 1872-9142 ] ; 2020.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Animaux, Antiviraux, Chameaux (génétique), Chameaux (immunologie), Clonage moléculaire, Escherichia coli, Interféron de type I (), Interféron de type I (génétique), Interféron de type I (immunologie), Interféron de type I (isolement et purification), Orthopoxvirus (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie).
- MESH :
- génétique : Chameaux, Interféron de type I, Protéines recombinantes.
- immunologie : Chameaux, Interféron de type I, Orthopoxvirus, Protéines recombinantes.
- isolement et purification : Interféron de type I.
- Analyse de séquence d'ADN, Animaux, Antiviraux, Clonage moléculaire, Escherichia coli, Interféron de type I.
English descriptors
- KwdEn :
- Animals, Antiviral Agents, Camelus (genetics), Camelus (immunology), Cloning, Molecular, Escherichia coli, Interferon Type I (chemistry), Interferon Type I (genetics), Interferon Type I (immunology), Interferon Type I (isolation & purification), Orthopoxvirus (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Sequence Analysis, DNA.
- MESH :
- chemical , chemistry : Interferon Type I.
- chemical , genetics : Interferon Type I, Recombinant Proteins.
- chemical , immunology : Interferon Type I, Recombinant Proteins.
- chemical , isolation & purification : Interferon Type I.
- chemical : Antiviral Agents.
- genetics : Camelus.
- immunology : Camelus, Orthopoxvirus.
- Animals, Cloning, Molecular, Escherichia coli, Sequence Analysis, DNA.
Abstract
Investigations into the molecular immune response of dromedary camel, a key livestock species of the arid, have been limited due to the lack of species-specific reagents. Here we describe for the first time, the identification and characterization of type I IFNs of dromedary camel, which are the most important cytokines in the innate host immune response against viruses. We cloned camel IFN-α coding sequences and identified a total of eleven subtypes. The canonical IFN-α subtype designated as IFN-α1 contained a 555-bp Open Reading Frame encoding a protein of 184 amino acids. Recombinant IFN-α1 protein was produced in E. coli and purified from inclusion bodies. Recombinant camel IFN-α1 induced the mRNA expression of interferon-stimulated genes (ISGs) in camel kidney cells. The purified protein also showed potent in-vitro antiviral activity against Camelpox Virus in kidney cells. The identified camel IFN-α protein and the subtypes will facilitate a better understanding of the host immune response to viral infections in camel and the development of potential antiviral biologicals for zoonotic diseases for which camel act as a reservoir.
DOI: 10.1016/j.molimm.2020.01.020
PubMed: 32014632
Affiliations:
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Le document en format XML
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Here we describe for the first time, the identification and characterization of type I IFNs of dromedary camel, which are the most important cytokines in the innate host immune response against viruses. We cloned camel IFN-α coding sequences and identified a total of eleven subtypes. The canonical IFN-α subtype designated as IFN-α1 contained a 555-bp Open Reading Frame encoding a protein of 184 amino acids. Recombinant IFN-α1 protein was produced in E. coli and purified from inclusion bodies. Recombinant camel IFN-α1 induced the mRNA expression of interferon-stimulated genes (ISGs) in camel kidney cells. The purified protein also showed potent in-vitro antiviral activity against Camelpox Virus in kidney cells. The identified camel IFN-α protein and the subtypes will facilitate a better understanding of the host immune response to viral infections in camel and the development of potential antiviral biologicals for zoonotic diseases for which camel act as a reservoir.</div></front></TEI><affiliations><list><country><li>Émirats arabes unis</li></country></list><tree><country name="Émirats arabes unis"><noRegion><name sortKey="Premraj, Avinash" sort="Premraj, Avinash" uniqKey="Premraj A" first="Avinash" last="Premraj">Avinash Premraj</name></noRegion><name sortKey="Aleyas, Abi George" sort="Aleyas, Abi George" uniqKey="Aleyas A" first="Abi George" last="Aleyas">Abi George Aleyas</name><name sortKey="Nautiyal, Binita" sort="Nautiyal, Binita" uniqKey="Nautiyal B" first="Binita" last="Nautiyal">Binita Nautiyal</name><name sortKey="Rasool, Thaha Jamal" sort="Rasool, Thaha Jamal" uniqKey="Rasool T" first="Thaha Jamal" last="Rasool">Thaha Jamal Rasool</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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