Intermolecular localization of epitopes within an oligomeric protein by immunoelectron microscopy and image processing
Identifieur interne : 004C62 ( Main/Curation ); précédent : 004C61; suivant : 004C63Intermolecular localization of epitopes within an oligomeric protein by immunoelectron microscopy and image processing
Auteurs : Nicolas Boisset [France] ; Joachim Frank [États-Unis] ; Jean Christophe Taveau [France] ; Philippe Billiald [France] ; Genevieve Motta [France] ; Josette Lamy [France] ; Pierre Yves Sizaret [France] ; Jean Lamy [France]Source :
- Proteins: Structure, Function, and Bioinformatics [ 0887-3585 ] ; 1988.
Abstract
Three epitopes have been localized by immunoelectron microscopy on subunits Aa6 of the 4 × 6‐meric hemocyanin of the scorpion Androctonus australis. Soluble immunocomplexes composed of monoclonal antibodies and of native hemocyanin were purified, negatively stained with uranyle acetate by the single‐layer technique, and examined under the electron microscope (EM). The molecule images were digitized, aligned, and submitted to correspondence analysis according to the method of Van Heel and Frank (Ultramicroscopy 6: 187‐194, 1981). A high‐precision localization of the attachment point of the Fab arm to the antigen was achieved through a careful analysis of the average images. This method easily allowed the discrimination of epitopes located in different domains (Mr 20 kDa) of the same subunit. Nonoverlapping epitopes located in the same structural domain of subunit Aa6 could be distinguished by the stain exclusion patterns of their Fab arms. The method is general and may be used for epitope mapping in any antigen producing definite EM views.
Url:
DOI: 10.1002/prot.340030305
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000176
- to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :000176
- to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002149
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :004D42
Links to Exploration step
ISTEX:4CC0865FB083372342E102B2BEE4CAB103C3DA93Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Intermolecular localization of epitopes within an oligomeric protein by immunoelectron microscopy and image processing</title>
<author><name sortKey="Boisset, Nicolas" sort="Boisset, Nicolas" uniqKey="Boisset N" first="Nicolas" last="Boisset">Nicolas Boisset</name>
</author>
<author><name sortKey="Frank, Joachim" sort="Frank, Joachim" uniqKey="Frank J" first="Joachim" last="Frank">Joachim Frank</name>
</author>
<author><name sortKey="Taveau, Jean Christophe" sort="Taveau, Jean Christophe" uniqKey="Taveau J" first="Jean Christophe" last="Taveau">Jean Christophe Taveau</name>
</author>
<author><name sortKey="Billiald, Philippe" sort="Billiald, Philippe" uniqKey="Billiald P" first="Philippe" last="Billiald">Philippe Billiald</name>
</author>
<author><name sortKey="Motta, Genevieve" sort="Motta, Genevieve" uniqKey="Motta G" first="Genevieve" last="Motta">Genevieve Motta</name>
</author>
<author><name sortKey="Lamy, Josette" sort="Lamy, Josette" uniqKey="Lamy J" first="Josette" last="Lamy">Josette Lamy</name>
</author>
<author><name sortKey="Sizaret, Pierre Yves" sort="Sizaret, Pierre Yves" uniqKey="Sizaret P" first="Pierre Yves" last="Sizaret">Pierre Yves Sizaret</name>
</author>
<author><name sortKey="Lamy, Jean" sort="Lamy, Jean" uniqKey="Lamy J" first="Jean" last="Lamy">Jean Lamy</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:4CC0865FB083372342E102B2BEE4CAB103C3DA93</idno>
<date when="1988" year="1988">1988</date>
<idno type="doi">10.1002/prot.340030305</idno>
<idno type="url">https://api.istex.fr/ark:/67375/WNG-1WQ926X9-Z/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000176</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000176</idno>
<idno type="wicri:Area/Istex/Curation">000176</idno>
<idno type="wicri:Area/Istex/Checkpoint">002149</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002149</idno>
<idno type="wicri:doubleKey">0887-3585:1988:Boisset N:intermolecular:localization:of</idno>
<idno type="wicri:Area/Main/Merge">004D42</idno>
<idno type="wicri:Area/Main/Curation">004C62</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Intermolecular localization of epitopes within an oligomeric protein by immunoelectron microscopy and image processing</title>
<author><name sortKey="Boisset, Nicolas" sort="Boisset, Nicolas" uniqKey="Boisset N" first="Nicolas" last="Boisset">Nicolas Boisset</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biochimie, Faculté de Pharmacie, Université François Rabelais, 37032 Tours Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Frank, Joachim" sort="Frank, Joachim" uniqKey="Frank J" first="Joachim" last="Frank">Joachim Frank</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
<wicri:cityArea>Wadsworth Center for Laboratories and Research, State of New York Department of Health, Albany</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Taveau, Jean Christophe" sort="Taveau, Jean Christophe" uniqKey="Taveau J" first="Jean Christophe" last="Taveau">Jean Christophe Taveau</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire Electronique, Université François Rabelais, 37032 Tours Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Billiald, Philippe" sort="Billiald, Philippe" uniqKey="Billiald P" first="Philippe" last="Billiald">Philippe Billiald</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biochimie, Faculté de Pharmacie, Université François Rabelais, 37032 Tours Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Motta, Genevieve" sort="Motta, Genevieve" uniqKey="Motta G" first="Genevieve" last="Motta">Genevieve Motta</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratorie d′Immunogéenétique, université d′Orleans, 45046 Orleans Cedex and CNRS Cesal, 45045 Orleans Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Orléans</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lamy, Josette" sort="Lamy, Josette" uniqKey="Lamy J" first="Josette" last="Lamy">Josette Lamy</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biochimie, Faculté de Pharmacie, Université François Rabelais, 37032 Tours Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sizaret, Pierre Yves" sort="Sizaret, Pierre Yves" uniqKey="Sizaret P" first="Pierre Yves" last="Sizaret">Pierre Yves Sizaret</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire Electronique, Université François Rabelais, 37032 Tours Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lamy, Jean" sort="Lamy, Jean" uniqKey="Lamy J" first="Jean" last="Lamy">Jean Lamy</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Biochimie, Faculté de Pharmacie, Université François Rabelais, 37032 Tours Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><country xml:lang="fr" wicri:curation="lc">France</country>
<wicri:regionArea>Correspondence address: Laboratoire de Biochimie, Faculté de Pharmacie, 2 Bis Boulevard Tonnelle, 37032 Tours Codex</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours Codex</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Proteins: Structure, Function, and Bioinformatics</title>
<title level="j" type="alt">PROTEINS: STRUCTURE, FUNCTION, AND BIOINFORMATICS</title>
<idno type="ISSN">0887-3585</idno>
<idno type="eISSN">1097-0134</idno>
<imprint><biblScope unit="vol">3</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="161">161</biblScope>
<biblScope unit="page" to="183">183</biblScope>
<biblScope unit="page-count">23</biblScope>
<publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher>
<pubPlace>Hoboken</pubPlace>
<date type="published" when="1988">1988</date>
</imprint>
<idno type="ISSN">0887-3585</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0887-3585</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Three epitopes have been localized by immunoelectron microscopy on subunits Aa6 of the 4 × 6‐meric hemocyanin of the scorpion Androctonus australis. Soluble immunocomplexes composed of monoclonal antibodies and of native hemocyanin were purified, negatively stained with uranyle acetate by the single‐layer technique, and examined under the electron microscope (EM). The molecule images were digitized, aligned, and submitted to correspondence analysis according to the method of Van Heel and Frank (Ultramicroscopy 6: 187‐194, 1981). A high‐precision localization of the attachment point of the Fab arm to the antigen was achieved through a careful analysis of the average images. This method easily allowed the discrimination of epitopes located in different domains (Mr 20 kDa) of the same subunit. Nonoverlapping epitopes located in the same structural domain of subunit Aa6 could be distinguished by the stain exclusion patterns of their Fab arms. The method is general and may be used for epitope mapping in any antigen producing definite EM views.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004C62 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 004C62 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Curation |type= RBID |clé= ISTEX:4CC0865FB083372342E102B2BEE4CAB103C3DA93 |texte= Intermolecular localization of epitopes within an oligomeric protein by immunoelectron microscopy and image processing }}
This area was generated with Dilib version V0.6.33. |