The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.
Identifieur interne : 003306 ( Main/Curation ); précédent : 003305; suivant : 003307The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.
Auteurs : Jarosław Przetakiewicz [Pologne] ; Anna Nadolska-Orczyk ; Wacław OrczykSource :
- Cellular & molecular biology letters [ 1425-8153 ] ; 2002.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA Primers.
- genetics : Solanum tuberosum.
- Base Sequence, Diploidy, Genetic Markers, Hybridization, Genetic, Random Amplified Polymorphic DNA Technique.
Abstract
Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.
PubMed: 12378226
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pubmed:12378226Le document en format XML
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<term>Hybridization, Genetic</term>
<term>Random Amplified Polymorphic DNA Technique</term>
<term>Solanum tuberosum (genetics)</term>
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<term>Diploïdie</term>
<term>Hybridation génétique</term>
<term>Marqueurs génétiques</term>
<term>Solanum tuberosum (génétique)</term>
<term>Séquence nucléotidique</term>
<term>Technique RAPD</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term>
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<term>Solanum tuberosum</term>
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<term>Diploidy</term>
<term>Genetic Markers</term>
<term>Hybridization, Genetic</term>
<term>Random Amplified Polymorphic DNA Technique</term>
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<term>Hybridation génétique</term>
<term>Marqueurs génétiques</term>
<term>Séquence nucléotidique</term>
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<front><div type="abstract" xml:lang="en">Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.</div>
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