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The highly conserved basic domain I of baculovirus IE1 is required for hr enhancer DNA binding and hr-dependent transactivation.

Identifieur interne : 003187 ( Main/Curation ); précédent : 003186; suivant : 003188

The highly conserved basic domain I of baculovirus IE1 is required for hr enhancer DNA binding and hr-dependent transactivation.

Auteurs : Victoria A. Olson [États-Unis] ; Justin A. Wetter ; Paul D. Friesen

Source :

Journal of virology [ 0022-538X ] ; 2003.

RBID : pubmed:12719559

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Cell Nucleus (metabolism), Cells, Cultured, Conserved Sequence, DNA, Viral (metabolism), DNA-Binding Proteins (chemistry), DNA-Binding Proteins (genetics), DNA-Binding Proteins (metabolism), Dimerization, Enhancer Elements, Genetic (genetics), Enhancer Elements, Genetic (physiology), Immediate-Early Proteins (chemistry), Immediate-Early Proteins (genetics), Immediate-Early Proteins (metabolism), Molecular Sequence Data, Mutagenesis, Insertional, Mutagenesis, Site-Directed, Nucleopolyhedroviruses (genetics), Nucleopolyhedroviruses (metabolism), Promoter Regions, Genetic, Spodoptera, Trans-Activators (chemistry), Trans-Activators (genetics), Trans-Activators (metabolism), Transcriptional Activation.
MESH :
- chemical , chemistry : DNA-Binding Proteins, Immediate-Early Proteins, Trans-Activators.
- chemical , genetics : DNA-Binding Proteins, Immediate-Early Proteins, Trans-Activators.
- chemical , metabolism : DNA, Viral, DNA-Binding Proteins, Immediate-Early Proteins, Trans-Activators.
- genetics : Enhancer Elements, Genetic, Nucleopolyhedroviruses.
- metabolism : Cell Nucleus, Nucleopolyhedroviruses.
- physiology : Enhancer Elements, Genetic.
- Amino Acid Sequence, Animals, Cells, Cultured, Conserved Sequence, Dimerization, Molecular Sequence Data, Mutagenesis, Insertional, Mutagenesis, Site-Directed, Promoter Regions, Genetic, Spodoptera, Transcriptional Activation.

Abstract

The immediate-early protein IE1 is the principal transcriptional regulator of the baculovirus Autographa californica nucleopolyhedrovirus (AcMNPV). Transactivation by IE1 is dramatically stimulated by cis linkage of the affected promoter to AcMNPV homologous region (hr) elements that contain palindromic 28-bp repeats (28-mers) with enhancer activity. This hr-dependent transcriptional enhancement requires binding of the 28-mer by dimeric IE1. Here, we have defined IE1 domains required for this DNA binding in order to investigate the mechanism of IE1 function. Analysis of a panel of IE1 insertion mutations indicated that disruption of a highly conserved domain (residues 152 to 161) consisting of mostly positive-charged residues (basic domain I) abolished hr-dependent transactivation. Targeted mutagenesis of basic residues within basic domain I caused loss of hr-dependent transactivation but had no effect on IE1 oligomerization, nuclear localization, or hr-independent transactivation of viral promoters. Alanine substitutions of K(152) and K(154) or K(160) and K(161) impaired IE1 binding to 28-mer DNA as a homodimer, indicating that these basic residues are required for enhancer binding. Consistent with a DNA-binding defect, 28-mer interaction was improved by heterodimerization with wild-type IE1 or by increasing mutated IE1 concentrations. DNA binding mediated by basic domain I was also required for IE1 transactivation that occurred through physically separated, unlinked hr elements. We concluded that basic domain I is the enhancer-binding domain for IE1. Our data also suggest that DNA binding activates IE1 for transcriptional enhancement, possibly through a conformational change involving basic domain I.

DOI: 10.1128/jvi.77.10.5668-5677.2003
PubMed: 12719559

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Le document en format XML

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<term>Conserved Sequence</term>
<term>DNA, Viral (metabolism)</term>
<term>DNA-Binding Proteins (chemistry)</term>
<term>DNA-Binding Proteins (genetics)</term>
<term>DNA-Binding Proteins (metabolism)</term>
<term>Dimerization</term>
<term>Enhancer Elements, Genetic (genetics)</term>
<term>Enhancer Elements, Genetic (physiology)</term>
<term>Immediate-Early Proteins (chemistry)</term>
<term>Immediate-Early Proteins (genetics)</term>
<term>Immediate-Early Proteins (metabolism)</term>
<term>Molecular Sequence Data</term>
<term>Mutagenesis, Insertional</term>
<term>Mutagenesis, Site-Directed</term>
<term>Nucleopolyhedroviruses (genetics)</term>
<term>Nucleopolyhedroviruses (metabolism)</term>
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<term>Trans-Activators (genetics)</term>
<term>Trans-Activators (metabolism)</term>
<term>Transcriptional Activation</term>
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<term>Activation de la transcription</term>
<term>Animaux</term>
<term>Cellules cultivées</term>
<term>Dimérisation</term>
<term>Données de séquences moléculaires</term>
<term>Mutagenèse dirigée</term>
<term>Mutagenèse par insertion</term>
<term>Noyau de la cellule (métabolisme)</term>
<term>Protéines de liaison à l'ADN ()</term>
<term>Protéines de liaison à l'ADN (génétique)</term>
<term>Protéines de liaison à l'ADN (métabolisme)</term>
<term>Protéines précoces immédiates ()</term>
<term>Protéines précoces immédiates (génétique)</term>
<term>Protéines précoces immédiates (métabolisme)</term>
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<term>Séquence d'acides aminés</term>
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<term>Transactivateurs (métabolisme)</term>
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<term>Immediate-Early Proteins</term>
<term>Trans-Activators</term>
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<term>Cells, Cultured</term>
<term>Conserved Sequence</term>
<term>Dimerization</term>
<term>Molecular Sequence Data</term>
<term>Mutagenesis, Insertional</term>
<term>Mutagenesis, Site-Directed</term>
<term>Promoter Regions, Genetic</term>
<term>Spodoptera</term>
<term>Transcriptional Activation</term>
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<term>Animaux</term>
<term>Cellules cultivées</term>
<term>Dimérisation</term>
<term>Données de séquences moléculaires</term>
<term>Mutagenèse dirigée</term>
<term>Mutagenèse par insertion</term>
<term>Protéines de liaison à l'ADN</term>
<term>Protéines précoces immédiates</term>
<term>Régions promotrices (génétique)</term>
<term>Spodoptera</term>
<term>Séquence conservée</term>
<term>Séquence d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">The immediate-early protein IE1 is the principal transcriptional regulator of the baculovirus Autographa californica nucleopolyhedrovirus (AcMNPV). Transactivation by IE1 is dramatically stimulated by cis linkage of the affected promoter to AcMNPV homologous region (hr) elements that contain palindromic 28-bp repeats (28-mers) with enhancer activity. This hr-dependent transcriptional enhancement requires binding of the 28-mer by dimeric IE1. Here, we have defined IE1 domains required for this DNA binding in order to investigate the mechanism of IE1 function. Analysis of a panel of IE1 insertion mutations indicated that disruption of a highly conserved domain (residues 152 to 161) consisting of mostly positive-charged residues (basic domain I) abolished hr-dependent transactivation. Targeted mutagenesis of basic residues within basic domain I caused loss of hr-dependent transactivation but had no effect on IE1 oligomerization, nuclear localization, or hr-independent transactivation of viral promoters. Alanine substitutions of K(152) and K(154) or K(160) and K(161) impaired IE1 binding to 28-mer DNA as a homodimer, indicating that these basic residues are required for enhancer binding. Consistent with a DNA-binding defect, 28-mer interaction was improved by heterodimerization with wild-type IE1 or by increasing mutated IE1 concentrations. DNA binding mediated by basic domain I was also required for IE1 transactivation that occurred through physically separated, unlinked hr elements. We concluded that basic domain I is the enhancer-binding domain for IE1. Our data also suggest that DNA binding activates IE1 for transcriptional enhancement, possibly through a conformational change involving basic domain I.</div>
</front>
</TEI>
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The highly conserved basic domain I of baculovirus IE1 is required for hr enhancer DNA binding and hr-dependent transactivation.

The highly conserved basic domain I of baculovirus IE1 is required for hr enhancer DNA binding and hr-dependent transactivation.

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Descripteurs français

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Abstract

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