MersV1, Main, Curation, bibRecord, 002E67

DNA hybridization detection on electrical microarrays using coulostatic pulse technique

Identifieur interne : 002E67 ( Main/Curation ); précédent : 002E66; suivant : 002E68

DNA hybridization detection on electrical microarrays using coulostatic pulse technique

Auteurs : V. Dharuman [Allemagne] ; E. Nebling [Allemagne] ; T. Grunwald [Allemagne] ; J. Albers [Allemagne] ; L. Blohm [Allemagne] ; B. Elsholz [Allemagne] ; R. Wörl [Allemagne] ; R. Hintsche [Allemagne]

Source :

Biosensors & bioelectronics [ 0956-5663 ] ; 2006.

RBID : Pascal:07-0277691

Descripteurs français

KwdFr :
- ADN (analyse), ADN (génétique), Analyse de panne d'appareillage, Conception d'appareillage, Hybridation in situ (), Hybridation in situ (instrumentation), Microélectrodes, Séquençage par oligonucléotides en batterie (), Séquençage par oligonucléotides en batterie (instrumentation), Électricité statique, Électrochimie (), Électrochimie (instrumentation).
MESH :
- analyse : ADN.
- génétique : ADN.
Pascal (Inist)
- Analyse de panne d'appareillage, Conception d'appareillage, Hybridation in situ, Hybridation moléculaire, Détection, Microélectrodes, Séquençage par oligonucléotides en batterie, Technique, Electrode, Impédance, Système sur puce, Puce à DNA, Biopuce, Électricité statique, Électrochimie.

English descriptors

KwdEn :
- Biochip, DNA (analysis), DNA (genetics), DNA chip, Detection, Electrochemistry (instrumentation), Electrochemistry (methods), Electrodes, Equipment Design, Equipment Failure Analysis, Impedance, In Situ Hybridization (instrumentation), In Situ Hybridization (methods), Microelectrodes, Molecular hybridization, Oligonucleotide Array Sequence Analysis (instrumentation), Oligonucleotide Array Sequence Analysis (methods), Static Electricity, System on a chip, Technique.
MESH :
- chemical , analysis : DNA.
- chemical , genetics : DNA.
- instrumentation : Electrochemistry, In Situ Hybridization, Oligonucleotide Array Sequence Analysis.
- methods : Electrochemistry, In Situ Hybridization, Oligonucleotide Array Sequence Analysis.
- Equipment Design, Equipment Failure Analysis, Microelectrodes, Static Electricity.

Abstract

We demonstrated a novel application of transient coulostatic pulse technique for the detection of label free DNA hybridization on nm-sized gold interdigitated ultramicroelectrode arrays (Au-IDA) made in silicon technology. The array consists of eight different positions with an Au-IDA pair at each position arranged on the Si-based Biochip. Immobilization of capture probes onto the Au-IDA was accomplished by self-assembling of thiol-modified oligonucleotides. Target hybridization was indicated by a change in the magnitude of the time dependant potential relaxation curve in presence of electroactive Fe(CN)₆^3- in the phosphate buffer solution. While complementary DNA hybridization showed 50% increase in the relaxation potential, the non-complementary DNA showed a negligible change. A constant behaviour was noted for all positions. The dsDNA specific intercalating molecule, methylene blue, was found to be enhancing the discrimination effect. The changes in the relaxation potential curves were further corroborated following the ELISA like experiments using ExtraAvidine alkaline phosphatase labelling and redox recycling of para-aminophenol phosphate at IDAs. The coulostatic pulse technique was shown to be useful for identifying DNA sequences from brain tumour gene CK20, human herpes simplex virus, cytomegalovirus, Epstein-Barr virus and M13 phage. Compared to the hybridization of short chain ONTs (27 mers), the hybridization of long chain M 13 phage DNA showed three times higher increase in the relaxation curves. The method is fast enough to monitor hybridization interactions in milli or microsecond time scales and is well suitable for miniaturization and integration compared to the common impedance techniques for developing capacitative DNA sensors.

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Le document en format XML

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<author><name sortKey="Worl, R" sort="Worl, R" uniqKey="Worl R" first="R." last="Wörl">R. Wörl</name>
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<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department ofBiotechnical Microsystems, Fraunhofer Institute for Silicon Technology, Fraunhoferstrasse 1</s1>
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<series><title level="j" type="main">Biosensors & bioelectronics</title>
<title level="j" type="abbreviated">Biosens. bioelectron.</title>
<idno type="ISSN">0956-5663</idno>
<imprint><date when="2006">2006</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biochip</term>
<term>DNA (analysis)</term>
<term>DNA (genetics)</term>
<term>DNA chip</term>
<term>Detection</term>
<term>Electrochemistry (instrumentation)</term>
<term>Electrochemistry (methods)</term>
<term>Electrodes</term>
<term>Equipment Design</term>
<term>Equipment Failure Analysis</term>
<term>Impedance</term>
<term>In Situ Hybridization (instrumentation)</term>
<term>In Situ Hybridization (methods)</term>
<term>Microelectrodes</term>
<term>Molecular hybridization</term>
<term>Oligonucleotide Array Sequence Analysis (instrumentation)</term>
<term>Oligonucleotide Array Sequence Analysis (methods)</term>
<term>Static Electricity</term>
<term>System on a chip</term>
<term>Technique</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term>
<term>ADN (génétique)</term>
<term>Analyse de panne d'appareillage</term>
<term>Conception d'appareillage</term>
<term>Hybridation in situ ()</term>
<term>Hybridation in situ (instrumentation)</term>
<term>Microélectrodes</term>
<term>Séquençage par oligonucléotides en batterie ()</term>
<term>Séquençage par oligonucléotides en batterie (instrumentation)</term>
<term>Électricité statique</term>
<term>Électrochimie ()</term>
<term>Électrochimie (instrumentation)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Electrochemistry</term>
<term>In Situ Hybridization</term>
<term>Oligonucleotide Array Sequence Analysis</term>
</keywords>
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<term>In Situ Hybridization</term>
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<front><div type="abstract" xml:lang="en">We demonstrated a novel application of transient coulostatic pulse technique for the detection of label free DNA hybridization on nm-sized gold interdigitated ultramicroelectrode arrays (Au-IDA) made in silicon technology. The array consists of eight different positions with an Au-IDA pair at each position arranged on the Si-based Biochip. Immobilization of capture probes onto the Au-IDA was accomplished by self-assembling of thiol-modified oligonucleotides. Target hybridization was indicated by a change in the magnitude of the time dependant potential relaxation curve in presence of electroactive Fe(CN)<sub>6</sub>
<sup>3-</sup>
 in the phosphate buffer solution. While complementary DNA hybridization showed 50% increase in the relaxation potential, the non-complementary DNA showed a negligible change. A constant behaviour was noted for all positions. The dsDNA specific intercalating molecule, methylene blue, was found to be enhancing the discrimination effect. The changes in the relaxation potential curves were further corroborated following the ELISA like experiments using ExtraAvidine alkaline phosphatase labelling and redox recycling of para-aminophenol phosphate at IDAs. The coulostatic pulse technique was shown to be useful for identifying DNA sequences from brain tumour gene CK20, human herpes simplex virus, cytomegalovirus, Epstein-Barr virus and M13 phage. Compared to the hybridization of short chain ONTs (27 mers), the hybridization of long chain M 13 phage DNA showed three times higher increase in the relaxation curves. The method is fast enough to monitor hybridization interactions in milli or microsecond time scales and is well suitable for miniaturization and integration compared to the common impedance techniques for developing capacitative DNA sensors.</div>
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<front><div type="abstract" xml:lang="en">We demonstrated a novel application of transient coulostatic pulse technique for the detection of label free DNA hybridization on nm-sized gold interdigitated ultramicroelectrode arrays (Au-IDA) made in silicon technology. The array consists of eight different positions with an Au-IDA pair at each position arranged on the Si-based Biochip. Immobilization of capture probes onto the Au-IDA was accomplished by self-assembling of thiol-modified oligonucleotides. Target hybridization was indicated by a change in the magnitude of the time dependant potential relaxation curve in presence of electroactive Fe(CN)<sub>6</sub>
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 in the phosphate buffer solution. While complementary DNA hybridization showed 50% increase in the relaxation potential, the non-complementary DNA showed a negligible change. A constant behaviour was noted for all positions. The dsDNA specific intercalating molecule, methylene blue, was found to be enhancing the discrimination effect. The changes in the relaxation potential curves were further corroborated following the ELISA like experiments using ExtraAvidine alkaline phosphatase labelling and redox recycling of para-aminophenol phosphate at IDAs. The coulostatic pulse technique was shown to be useful for identifying DNA sequences from brain tumour gene CK20, human herpes simplex virus, cytomegalovirus, Epstein-Barr virus and M13 phage. Compared to the hybridization of short chain ONTs (27 mers), the hybridization of long chain M 13 phage DNA showed three times higher increase in the relaxation curves. The method is fast enough to monitor hybridization interactions in milli or microsecond time scales and is well suitable for miniaturization and integration compared to the common impedance techniques for developing capacitative DNA sensors.</div>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">DNA hybridization detection on electrical microarrays using coulostatic pulse technique.</title>
<author><name sortKey="Dharuman, V" sort="Dharuman, V" uniqKey="Dharuman V" first="V" last="Dharuman">V. Dharuman</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnical Microsystems, Fraunhofer Institute for Silicon Technology, Fraunhoferstrasse 1, D-25524 Itzehoe, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Biotechnical Microsystems, Fraunhofer Institute for Silicon Technology, Fraunhoferstrasse 1, D-25524 Itzehoe</wicri:regionArea>
<wicri:noRegion>25524 Itzehoe</wicri:noRegion>
<wicri:noRegion>25524 Itzehoe</wicri:noRegion>
<wicri:noRegion>D-25524 Itzehoe</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nebling, E" sort="Nebling, E" uniqKey="Nebling E" first="E" last="Nebling">E. Nebling</name>
</author>
<author><name sortKey="Grunwald, T" sort="Grunwald, T" uniqKey="Grunwald T" first="T" last="Grunwald">T. Grunwald</name>
</author>
<author><name sortKey="Albers, J" sort="Albers, J" uniqKey="Albers J" first="J" last="Albers">J. Albers</name>
</author>
<author><name sortKey="Blohm, L" sort="Blohm, L" uniqKey="Blohm L" first="L" last="Blohm">L. Blohm</name>
</author>
<author><name sortKey="Elsholz, B" sort="Elsholz, B" uniqKey="Elsholz B" first="B" last="Elsholz">B. Elsholz</name>
</author>
<author><name sortKey="Worl, R" sort="Worl, R" uniqKey="Worl R" first="R" last="Wörl">R. Wörl</name>
</author>
<author><name sortKey="Hintsche, R" sort="Hintsche, R" uniqKey="Hintsche R" first="R" last="Hintsche">R. Hintsche</name>
</author>
</analytic>
<series><title level="j">Biosensors & bioelectronics</title>
<idno type="ISSN">0956-5663</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA (analysis)</term>
<term>DNA (genetics)</term>
<term>Electrochemistry (instrumentation)</term>
<term>Electrochemistry (methods)</term>
<term>Equipment Design</term>
<term>Equipment Failure Analysis</term>
<term>In Situ Hybridization (instrumentation)</term>
<term>In Situ Hybridization (methods)</term>
<term>Microelectrodes</term>
<term>Oligonucleotide Array Sequence Analysis (instrumentation)</term>
<term>Oligonucleotide Array Sequence Analysis (methods)</term>
<term>Static Electricity</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term>
<term>ADN (génétique)</term>
<term>Analyse de panne d'appareillage</term>
<term>Conception d'appareillage</term>
<term>Hybridation in situ ()</term>
<term>Hybridation in situ (instrumentation)</term>
<term>Microélectrodes</term>
<term>Séquençage par oligonucléotides en batterie ()</term>
<term>Séquençage par oligonucléotides en batterie (instrumentation)</term>
<term>Électricité statique</term>
<term>Électrochimie ()</term>
<term>Électrochimie (instrumentation)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Electrochemistry</term>
<term>In Situ Hybridization</term>
<term>Oligonucleotide Array Sequence Analysis</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrochemistry</term>
<term>In Situ Hybridization</term>
<term>Oligonucleotide Array Sequence Analysis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Equipment Design</term>
<term>Equipment Failure Analysis</term>
<term>Microelectrodes</term>
<term>Static Electricity</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Analyse de panne d'appareillage</term>
<term>Conception d'appareillage</term>
<term>Hybridation in situ</term>
<term>Microélectrodes</term>
<term>Séquençage par oligonucléotides en batterie</term>
<term>Électricité statique</term>
<term>Électrochimie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We demonstrated a novel application of transient coulostatic pulse technique for the detection of label free DNA hybridization on nm-sized gold interdigitated ultramicroelectrode arrays (Au-IDA) made in silicon technology. The array consists of eight different positions with an Au-IDA pair at each position arranged on the Si-based Biochip. Immobilization of capture probes onto the Au-IDA was accomplished by self-assembling of thiol-modified oligonucleotides. Target hybridization was indicated by a change in the magnitude of the time dependant potential relaxation curve in presence of electroactive Fe(CN)(6)(3-) in the phosphate buffer solution. While complementary DNA hybridization showed 50% increase in the relaxation potential, the non-complementary DNA showed a negligible change. A constant behaviour was noted for all positions. The dsDNA specific intercalating molecule, methylene blue, was found to be enhancing the discrimination effect. The changes in the relaxation potential curves were further corroborated following the ELISA like experiments using ExtraAvidine alkaline phosphatase labelling and redox recycling of para-aminophenol phosphate at IDAs. The coulostatic pulse technique was shown to be useful for identifying DNA sequences from brain tumour gene CK20, human herpes simplex virus, cytomegalovirus, Epstein-Barr virus and M13 phage. Compared to the hybridization of short chain ONTs (27 mers), the hybridization of long chain M13 phage DNA showed three times higher increase in the relaxation curves. The method is fast enough to monitor hybridization interactions in milli or microsecond time scales and is well suitable for miniaturization and integration compared to the common impedance techniques for developing capacitative DNA sensors.</div>
</front>
</TEI>
</PubMed>
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DNA hybridization detection on electrical microarrays using coulostatic pulse technique

DNA hybridization detection on electrical microarrays using coulostatic pulse technique

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